Influence of Nail Varnish on the  Remineralization of Enamel Single  Sections Assessed by Microradiography and Confocal Laser Scanning Microscopy

Iijima, Youichi; Takagi, Okiuji; Duschner, H.; Ruben, J.; Arends, J

doi:10.1159/000016476

Cited by 13 publications

(14 citation statements)

References 17 publications

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“…Using the thin section technique mentioned [Iijima et al, 1998] mineral changes with time were assessed. The experimental flow diagram is presented in figure 1.…”

Section: Methodsmentioning

confidence: 99%

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In vitro Remineralization of in vivo and in vitro Formed Enamel Lesions

et al. 1999

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Thin sections of natural white spot enamel lesions (WS) and of artificial in vitro lesions (VL) were remineralized simultaneously in vitro. The sections, clamped in a PMMA holder, were microradiographed at baseline and after remineralization in a calcium– and phosphate–containing solution (pH = 7.0; 1 ppm F) after 2 and 4 weeks. All data were analyzed with respect to baseline. The results show that the lesion depth values did not change significantly during 2 and 4 weeks of remineralization. The mineral accumulation (change in ΔZ), however, was substantial and significant in WS and VL. In WS the change in mineral accumulation was roughly proportional to the amount of mineral at baseline. The WS accumulated more than two times the amount of mineral than VL in the same periods of remineralization. After 4 weeks of remineralization the maximum mineral value V_max in the surface layer of the WS was nearly up to the sound enamel level ≈87 vol%. This study shows that the technique and calculation procedure described make this single section method attractive for longitudinal demineralization–remineralization studies in vitro or in situ. Both WS and VL samples obviously remineralized in vitro similarly with respect to the baseline. Furthermore, this in vitro work indicates that remineralization inhibitors present in saliva, and previously penetrated into the enamel tissue, do not influence remineralization later on.

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“…Using the thin section technique mentioned [Iijima et al, 1998] mineral changes with time were assessed. The experimental flow diagram is presented in figure 1.…”

Section: Methodsmentioning

confidence: 99%

“…According to Iijima et al [1998], no difference in remineralization efficacy between lesions in bulk samples and lesions in thin sections clamped in a polymethylmethacrylate (PMMA) holder exists. This indicates that by using single sections one can also conveniently and longitudinally investigate the remineralization of in vivo formed WS.…”

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confidence: 99%

In vitro Remineralization of in vivo and in vitro Formed Enamel Lesions

et al. 1999

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“…To overcome this disadvantage, some researchers used a 'single thin section technique', where incipient lesion is artifi cially produced in the enamel section with a thickness of approx. 100 μm and monitored by TMR analysis 6) , to study the longitudinal mineral change by applying TMR method. However, the section of specimen for this method was fragile.…”

Section: Introductionmentioning

confidence: 99%

“…Dowker et al reported the development of subsurface enamel lesions using micro-CT 5) . Huang et al showed mineral density profi le of enamel white spot lesions using micro-CT 6) . However, they did not investigate longitudinal change in remineralization or longitudinal process of mineral change probably due to diffi culty with accurate alignment of the 3D images.…”

Section: Introductionmentioning

confidence: 99%

An approach to normalizing micro-CT depth profiles of mineral density for monitoring enamel remineralization progress

et al. 2012

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To indicate the possibility of a new approach to creating mineral density profi les, and to examine longitudinal changes in 'the rate of remineralization (RA)' and 'the mineral density (DAs) at 4 different depths' (surface zone: SZ, lesion body: LB, middle zone: MZ, deep zone near to sound area: DZ) in enamel subsurface lesions, eight demineralized bovine enamel-dentin blocks were remineralized for 1 to 4 week and investigated using Micro-focus X-ray CT (micro-CT). After CT scanning, mineral density profi les were created. Mineral densities at each depth after demineralization were SZ≅LBMZ>DZ. This study indicated a new approach to create a mineral density profi le and suggested the greater the value of the mineral density before the remineralization, the smaller the mineral density increments.

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“…Crucial to this technique is the durable and impenetrable coating of all surfaces with the exception of a narrow surface window. For this purpose nail varnish has been used for enamel or dentin [Creanor et al, 1986;Wefel and Jensen, 1992;Inaba et al, 1995], and also plastic sheets, epoxy resin or light-curing resin for enamel [Mellberg et al, 1986;Iijima et al, 1998;Exterkate et al, 1993;Tanaka et al, 1993]. Since dentin sections are likely to dehydrate or absorb water, we chose embedding in bonding agent rather than nail varnish.…”

Section: Discussionmentioning

confidence: 99%

Effect of a Solution with High Fluoride Concentration on Remineralization of Shallow and Deep Root Surface Caries in vitro

2001

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A root dentin single–section model was developed to compare the efficacy of different fluoride treatments on the remineralization of lesions. Shallow (∼170 µm) and deep (∼400 µm) lesions were produced in acetic acid buffer solutions (pH 5.0) with 0.1– and 0.5–ppm fluoride for 3 days and 2 weeks, respectively. Next, the sections were pH–cycled for 4 weeks. Following the pH cycling, all sections were first remineralized for 5 weeks, then subjected to 10 days of demineralization. The treatments were (1) no treatment (control), (2) daily 1,450–ppm NaF toothpaste, (3) weekly 4,000–ppm fluoride solution, (4) a combination of treatments 2 and 3. In the shallow lesions, the 4,000–ppm fluoride solution and the combination treatment enhanced mineral deposition at the lesion front, producing a second, slightly hyperremineralized layer. Similarly, in the deep lesions a second remineralized layer was detected. In all lesions, the fluoride solution treatment showed significantly more remineralization compared to the control and the toothpaste treatment (p<0.05), most of which was formed during the pH cycling. In the demineralization period, the control showed significantly higher mineral loss than all other treatments. These results indicate that a 4,000–ppm fluoride solution might be effective for remineralization of root dentin lesions even over 400 µm depth.

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Influence of Nail Varnish on the Remineralization of Enamel Single Sections Assessed by Microradiography and Confocal Laser Scanning Microscopy

Cited by 13 publications

References 17 publications

In vitro Remineralization of in vivo and in vitro Formed Enamel Lesions

In vitro Remineralization of in vivo and in vitro Formed Enamel Lesions

An approach to normalizing micro-CT depth profiles of mineral density for monitoring enamel remineralization progress

Effect of a Solution with High Fluoride Concentration on Remineralization of Shallow and Deep Root Surface Caries in vitro

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