Demonstration of a Fluoride Dose Response with an In situ Single-Section Dental Caries Model

Damato, F.A.; Stephen, K.W.

doi:10.1159/000261986

Cited by 8 publications

(9 citation statements)

References 22 publications

(26 reference statements)

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“…6 Another study has shown the relevance of plaque disturbance in the arrest of incipient carious lesions, regardless of the use of fluoride. 7 Most results, however, were obtained from in situ studies 4,5,8,9 and only a few of the studies were RCT, 6,7 which is recognized to be the most appropriate tool for comparing therapies. Further research on non-surgical treatment of incipient carious lesions is still needed.…”

Section: Declaration Of Interestsmentioning

confidence: 99%

Efficacy of 1.23% APF gel applications on incipient carious lesions: a double-blind randomized clinical trial

et al. 2013

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The aim of this double-blind randomized clinical trial was to evaluate the efficacy of 1.23% APF gel application on the arrest of active incipient carious lesions in children. Sixty 7-to 12-year-old children, with active incipient lesions were included in the study. Children were divided randomly into 2 groups: 1.23% APF gel and placebo gel applications. Each group received 8 weekly applications of treatment. The lesions were re-evaluated at the 4 th and 8 th appointments. Poisson regression analysis was used to estimate relative risks of the presence of active white spot lesions. Groups showed similar results (PR = 1.67; CI 95% 0.69-3.98). The persistence of at least 1 active lesion was associated with a higher number of lesions in the baseline (PR = 2.67; CI 95% 1.19-6.03), but not with sugar intake (PR = 1.06; CI 95% 0.56-2.86) and previous exposure to fluoride dentifrice (PR = 1.26; CI 95% 0.49-2.29). The trial demonstrates the equivalence of the treatments. The use of the APF gel showed no additional benefits in this sample of children exposed to fluoridated water and dentifrice. The professional dental plaque removal in both groups may also account for the resulting equivalence of the treatments.

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Section: Declaration Of Interestsmentioning

confidence: 99%

Efficacy of 1.23% APF gel applications on incipient carious lesions: a double-blind randomized clinical trial

et al. 2013

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“…Several authors [von Bartheld, 1980;Featherstone and Silverstone, 1982;Harvey et al, 1982;ten Cate and Exterkate, 1986;Mellberg et al, 1986;Creanor et al, 1986;Wefel et al, 1987;Strang et al, 1987;1988;Stephen et al, 1992;Lammers et al, 1990;Exterkate et al, 1993;Damato and Stephen, 1994] employed various varnishes and adhesives to prepare 'windows' and/or to cover cut surfaces. To 398 Caries Res 1998;32:393-400 Iijima/Takagi/Duschner/Ruben/Arends the authors' knowledge no previous work on the importance of NV penetration has been reported.…”

Section: Nv Penetrationmentioning

confidence: 99%

“…Single-section techniques have been used to investigate demineralization and remineralization of enamel and dentine in vitro [Featherstone and Silverstone, 1982;Harvey et al, 1982;ten Cate and Exterkate, 1986;Strang et al, 1988;Lammers et al, 1990;Exterkate et al, 1993;Inaba et al, 1995] as well as in situ [Creanor et al, 1986;Mellberg et al, 1986Mellberg et al, , 1992Mellberg, 1992;Wefel et al, 1987;Wefel, 1990;Strang et al, 1987;Stephen et al, 1992;Damato and Stephen, 1994]. Two types of single-section techniques are currently used to create lesions in enamel or dentine.…”

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confidence: 99%

“…Another aspect when thin sections are employed is the coating or sealing of exposed section sides. Nail varnish (NV) is most widely used to protect cut surfaces of thin sections in a demineralization or remineralization experiment [Creanor et al, 1986;ten Cate and Exterkate, 1986;Strang et al, 1988;Wefel et al, 1987;Stephen et al, 1992;Damato and Stephen, 1994].…”

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Influence of Nail Varnish on the Remineralization of Enamel Single Sections Assessed by Microradiography and Confocal Laser Scanning Microscopy

Iijima

Takagi²,

Duschner³

et al. 1998

Caries Res

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Single–section techniques are attractive in enamel de– and remineralization investigations because they allow longitudinal studies in which mineral changes can be assessed by microradiography (TMR). Nail varnish (NV) is in general applied to coat the cut thin–section sides. The aims of this study were to investigate: (1) NV penetration depth in cut surfaces of demineralized enamel, (2) the influence of NV on cut surfaces of demineralized enamel on TMR, (3) the influence of NV penetration on a following remineralization. Cut surfaces of thin sections of demineralized enamel were NV coated; the NV was peeled off and the penetration depth assessed by confocal laser scanning microscopy. The NV penetration was 18±5 μm (mean±SD) in demineralized enamel. To evaluate the possible influence of NV on TMR, cut surfaces of thin sections of demineralized enamel were coated (twice) and microradiographed before and after nail varnishing. The NV effect (total effect of penetrated and surface NV) on the main parameters of TMR, Ld and ΔZ, was less than 5% of the mean values. In the remineralization experiment (remineralization with 1.5 mM Ca²⁺, 0.9 mM phosphate, pH 7, 1 ppm F for 1 and 2 weeks), lesions in bulk samples, lesions in thin sections with NV–coated cut surfaces and lesions in thin sections in a PMMA (polymethylmethacrylate) holder were compared. (1) The remineralization of bulk samples and of NV–coated thin sections is different in one aspect. The amounts of mineral deposited in the lesions expressed as ΔZ are comparable after 1 week. But because the NV penetrates part of the lesion outside, there was an Ld difference. The lesion depth difference between bulk lesions and NV–coated lesions in thin sections was statistically significant and was about 19% less in NV–coated lesions after 1 week; after 2 weeks of remineralization there was no difference in Ld between bulk– and NV–coated lesions any more. (2) There was no difference in remineralization efficacy between lesions in bulk samples and lesions in thin sections in the PMMA holder.

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“…Each trough contains two thin sections of enamel with artificial lesions. Further details of this model may be found else where [Creanor et al, 1986b], This model has shown the ability to demonstrate remineralisation [Creanor et al, 1992], demineralisation [Creanor et al, 1994] and a fluoride dose response [Damato and Stephen, 1994],The trough in the lingual flange provides an excellent site for plaque accumulation. Two studies have been under taken [Creanor et al, 1986a;Macpherson et al, 1991] which have demonstrated no significant difference in microbial composition between the plaque in the appliance trough and adjacent plaque on the lingual surface of the lower molar teeth.…”

mentioning

confidence: 99%

Determination of Plaque pH Changes within the Trough of an in situ Appliance Used to Study Mineral Changes in Early Carious Lesions

Hall

Creanor²,

Strang³

et al. 1997

Caries Res

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Plaque pH was measured using a glass combination pH microelectrode of plaque deposits lining the trough of an intra-oral appliance which contained single sections of human teeth with artificial enamel lesions. Plaque exposure to a 10% sucrose solution produced significantly lower plaque pH levels than a solution of sorbitol gum extract. The subsequent flow of saliva over the plaque/pH electrode interface resulted in a significant and rapid increase in pH. Repeat experiments, using deionised water instead of saliva, demonstrated no increase in pH at the plaque/electrode interface. This system may provide a convenient method of producing plaque suitable for repeated pH analysis in intimate association with artificial enamel lesions. This may permit further study of the relationship between plaque acidogenicity, salivary buffering capacity and mineral change within the incipient enamel lesion.

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Demonstration of a Fluoride Dose Response with an In situ Single-Section Dental Caries Model

Cited by 8 publications

References 22 publications

Efficacy of 1.23% APF gel applications on incipient carious lesions: a double-blind randomized clinical trial

Efficacy of 1.23% APF gel applications on incipient carious lesions: a double-blind randomized clinical trial

Influence of Nail Varnish on the Remineralization of Enamel Single Sections Assessed by Microradiography and Confocal Laser Scanning Microscopy

Determination of Plaque pH Changes within the Trough of an in situ Appliance Used to Study Mineral Changes in Early Carious Lesions

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