Development of an immunochromatographic assay for rapid detection of 1‐Aminohydantoin in urine specimens

Abstract

A rapid immunochromatographic assay was developed and validated for detection of 1-aminohydantoin (AHD) in urine specimens. Colloidal gold-labeled polyclonal antibody specific to AHD derivative was used as the marker; based on the competitive reactivity theory, the metabolite of nitrofurantoin after derivatization with benzaldehyde would compete with carboxyphenyl AHD derivative-conjugated ovalbumin. The test strip could efficaciously detect the novel analyte with a visual detection limit of 10 ng mL(-1) and high specificity. The reliability of the assay was determined by testing 80 standard samples comparing with enzyme-linked immunosorbent assay. The semi-quantitative detection was accomplished in less than 15 min with low cost, especially for requirements of rapid and simple screening. This is the first publication of an immunochromatographic assay for detection of nitrofuran residues.

Development of an enzyme-linked immunosorbent assay for the detection of nitrofurantoin metabolite, 1-amino-hydantoin, in animal tissues

“…Some immunoassays that detect AOZ residue (Cheng et al, 2009;Cooper, Caddell, Elliott, & Kennedy, 2004;Vass et al, 2005), SEM (Cooper et al, 2007;Gao, Chen, Cheng, Lei, & Zeng, 2007) and AMOZ (Pimpitak et al, 2009) residues have already been reported. In addition, the detection of the nitrofuran parent drugs has also been reported (Li, Liu, & Wang, 2009;Liu et al, 2007;Xu et al, 2009). However, to the best of our knowledge no ELISA method has been reported to detect AHD residue in animal tissues.…”

An immunochromatographic assay for rapid and direct detection of 3‐amino‐5‐morpholino‐2‐oxazolidone (AMOZ) in meat and feed samples

“…Compared to other methods, ICA offers many advantages such as no requirement for instruments, simple operation, cheap price of detection and easy result evaluation. To date, for nitrofurans, there have been only three ICAs published for the detection of 1‐aminohydantoin (AHD, the metabolite of nitrofurantoin) and semicarbazide (SEM, the metabolite of nitrofurazone) . Nevertheless, in these three ICAs, the analyte is the derivative of AHD or SEM rather than the metabolite itself, e.g.…”

Development of a Lateral Flow Immunoassay (LFA) Strip for the Rapid Detection of 1‐Aminohydantoin in Meat Samples

“…However, because of the low cost, the use of nitrofurans has been resumed in recent years, which has raised concerns on safety of meat products. For example, in the European market, nitrofuran residues have been detected constantly in poultry and aquaculture products that were exported from Southeast Asia and Latin America (Xu and others 2009). …”