An immunochromatographic assay for rapid and direct detection of 3‐amino‐5‐morpholino‐2‐oxazolidone (<scp>AMOZ</scp>) in meat and feed samples

Li, Shuqun; Song, Juan; Yang, Hong; Cao, Biyun; Chang, Huafang; Deng, Anping

doi:10.1002/jsfa.6423

Cited by 18 publications

(14 citation statements)

References 33 publications

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“…The quantitative LOD of 2-NP-AMOZ was 0.74 ng/mL, which corresponded to AMOZ at a concentration of 0.45 ng/mL after calculation by the following formula: concentration of AMOZ = (molecular weight of AMOZ/ molecular weight of 2-NP-AMOZ) × concentration of 2-NP-AMOZ. The use of QDs improved the sensitivity of immunostrip compared to the colloidal gold-based immunostrip, according to what observed for AMOZ detection by previously reported in the literature (Li et al, 2014).…”

Section: Characterization Of Qds and Qds-mab Conjugatessupporting

confidence: 81%

“…The synthesis of the AMOZ derivatives was performed as described previously with slight modification (Li et al, 2014). Briefly, 1.5 mmol of 4-carboxybenzaldehyle was dissolv in 5 mL dried methanol, followed by the addition of 1.0 mmol AMOZ.…”

Section: Preparation Of Amoz Derivatives and Immunogensmentioning

confidence: 99%

“…During the past few decades, a colloidal gold nanoparticle-based immunochromatographic strip (immunostrip) has been published for the detection AMOZ (Li et al, 2014). Compared to other methods, immunostrip offers many advantages such as procedure simplicity, rapid, cheap, onsite and no requirement for expensive equipment (Li et al, 2014). However, use of the traditional fluorophore-gold nanoparticle based immunostrip for AMOZ detection is always limited by its relatively low detection sensitivity.…”

Section: Introductionmentioning

confidence: 99%

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A ﬂuorescent immunochromatographic strip using quantum dots for 3-amino-5-methylmorpholino-2-oxazolidinone (AMOZ) detection in edible animal tissues

Xie

Shi

et al. 2019

Food and Agricultural Immunology

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To monitor the levels of furaltadone in animal tissues, an immunochromatographic strip (immunostrip) based on quantum dot was developed for rapid detection of 3-amino-5methylmorpholino-2-oxazolidinone (AMOZ), the metabolite of furaltadone. Four monoclonal antibodies (mAbs) against nitrophenyl derivative of AMOZ (2-NP-AMOZ) were generated from the hybridoma cell lines. Among them, the mAb 5C10/3A8 showed the highest sensitivity to 2-NP-AMOZ. Using an immunostrip reader, with the 2-NP-AMOZ as the target, the immunostrip had a liner range from 1 to 30 ng/mL, with 50% inhibition concentration of 5.22 ng/mL and limit of detection of 0.07 ng/mL. The recoveries for AMOZ ranged from 78.6% to 108.9%, with coefficients of variation below 12%. A good correlation (R 2 = 0.9901) between the immunostrip and LC-MS/MS results in the tissues tested demonstrated the reliability of the immunostrip. These results indicate that proposed immunostrip is suitable for the rapid and quantitative detection of AMOZ residues in edible animal tissues. ARTICLE HISTORY

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Section: Characterization Of Qds and Qds-mab Conjugatessupporting

confidence: 81%

Section: Preparation Of Amoz Derivatives and Immunogensmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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A ﬂuorescent immunochromatographic strip using quantum dots for 3-amino-5-methylmorpholino-2-oxazolidinone (AMOZ) detection in edible animal tissues

Xie

Shi

et al. 2019

Food and Agricultural Immunology

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“…The extraction steps required generally depend upon the specificity and sensitivity of the antibody for the target in question and the matrix under analysis. Some published methods [18,19,23,26,45,55,56], especially for immunochromatographic and biosensor applications, use no sample derivatisation. In these cases the authors claim that antibody sensitivity and specificity for the underivatised nitrofuran metabolites is sufficient such that, when methods are calibrated with underivatised standards, detection can be achieved to meet the MRPL.…”

Section: Sample Preparationmentioning

confidence: 99%

Development of Antibodies and Immunoassays for Monitoring of Nitrofuran Antibiotics in the Food Chain

Cooper

Fodey

Campbell

et al. 2018

COC

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Nitrofurans are a broad group of drugs once widely used for treatment of microbial and protozoal infections in many livestock species. However, as concerns grew globally with regard to the potentially carcinogenic and mutagenic effects of their residues in foods, they have been banned from use in many parts of the world. In order to monitor compliance to these bans it is essential to have fitfor-purpose testing methods. Immunoassays are the screening tool of choice for many testing laboratories due to their relative low cost, ease of use and high sensitivity. As is the case with all immunoassays, the most important reagents required are high quality, high affinity antibodies that exhibit the required sensitivity and specificity. Generating such antibodies for the nitrofuran family of compounds has required a great deal of effort in the design of immunogens, as the compounds, due to size, are not capable of eliciting an immune response in hosts and are not easily conjugated to carrier proteins. This article reviews the range of strategies used to successfully generate suitable antibodies to a wide range of these drugs and their metabolites. In addition, the platform technologies for nitrofuran detection have moved from simple enzyme-linked immunosorbent assay (ELISA)-based procedures to more sophisticated multiplexing systems which can undertake faster and broader spectrum testing for the parent drugs and their metabolites. Reviews of the technologies used for immunochemical detection of the nitrofurans and of commercially available test kits are also presented.

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“…The ICA detection was completed within 10 min. 24 Wang et al established multiplex ICA using gold nanoparticles for the rapid and simultaneous detection of four nitrofuran metabolites in sh samples. The multi-ICA detection was accomplished in less than 15 min by the naked eye.…”

mentioning

confidence: 99%

Ultrasensitive strips for the quadruple detection of nitrofuran metabolite residues

Sun

Zhu

et al. 2019

RSC Adv.

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An immunochromatographic assay for rapid and direct detection of 3‐amino‐5‐morpholino‐2‐oxazolidone (AMOZ) in meat and feed samples

Abstract: The proposed ICA provides a feasible tool for simple, sensitive, rapid, convenient and semi-quantitative detection of AMOZ in meat and feed samples on site. To our knowledge, this is the first report of the ICA for direct detection of AMOZ.

Cited by 18 publications

References 33 publications

A ﬂuorescent immunochromatographic strip using quantum dots for 3-amino-5-methylmorpholino-2-oxazolidinone (AMOZ) detection in edible animal tissues

A ﬂuorescent immunochromatographic strip using quantum dots for 3-amino-5-methylmorpholino-2-oxazolidinone (AMOZ) detection in edible animal tissues

Development of Antibodies and Immunoassays for Monitoring of Nitrofuran Antibiotics in the Food Chain

Ultrasensitive strips for the quadruple detection of nitrofuran metabolite residues

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