Development of an Assay Method for Simultaneous Determination of Ciprofloxacin and Naproxen by UV Spectrophotometric Method

Nijhu, Rajia Sultana; Jhanker, Yeakuty Marzan; Sutradhar, Kumar Bishwajit

doi:10.3329/sjps.v4i1.8876

Cited by 15 publications

(9 citation statements)

References 9 publications

(2 reference statements)

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“…In this study ciprofloxacin hydrochloride tablets were assayed by using UV spectroscopic technique due to lack of instrumentation. A number of literatures suggest the UV spectroscopy for the analysis of ciprofloxacin hydrochloride tablet [ 49 ]. In fact there is no major problem in the assay of ciprofloxacin tablet by UV spectroscopic technique instead of high performance liquid chromatography technique.…”

Section: Discussionmentioning

confidence: 99%

In vitro quality evaluation of leading brands of ciprofloxacin tablets available in Bangladesh

et al. 2017

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BackgroundCiprofloxacin is a broad-spectrum antibiotic that acts against a number of bacterial infections. The study was carried out to examine the in vitro quality control tests for ten leading brands of ciprofloxacin hydrochloride 500 mg tablet formulation, registered in Bangladesh by Directorate General of Drug Administration. The quality control parameters of ten different brands of ciprofloxacin hydrochloride 500 mg tablets were determined by weight variation, friability, hardness, disintegration, dissolution and assay tests. All the tablets were evaluated for conformity with United States Pharmacopoeia-National Formulary (USP-NF) and British Pharmacopoeia (BP) standards.ResultsAmong ten brands of tablets Brand C had lower mean weight variation of 1.59% and Brand E had highest mean weight variation of 3.32%. For friability test Brand F had lowest mean friability (0.27%) and Brand G had highest mean friability (0.54%). Among ten brands mean lowest and highest hardness were founded in Brand G (4.49 kg/cm2) and Brand F (7.13 kg/cm2) respectively. The disintegration time for ten brands of ciprofloxacin tablet obtained were in the subsequent order: Brand G (8.19 min) < Brand C (9.25 min) < Brand E (9.61 min) < Brand D (10.11 min) < Brand B (11.07 min) < Brand A (12.15 min) < Brand H (13.68 min) < Brand I (14.59 min) < Brand J (16.32 min) < Brand F (17.49 min). Among ten brands for dissolution test mean percentages of drug release were not less than 80% in 45 min for four tablets (Brand E, 81.52%; Brand D, 86.44%; Brand G, 86.82% and Brand C, 94.12%), consequently they met BP standard and as per USP-NF standard six brands (Brand B, 75.62%; Brand A, 76.18%; Brand E, 81.52%; Brand D, 86.44%; Brand G, 86.82% and Brand C, 94.12%) had released not less than 75% drugs, so they also complied with the standard. The percentages of the drug content of the ten brands of ciprofloxacin tablet were obtained in the following sequence: Brand H (96.84%) < Brand J (97.34%) < Brand D (98.15%) < Brand I (98.47%) < Brand E (99.37%) < Brand F (100.28%) < Brand B (100.38%) < Brand A (100.54%) < Brand G (101.39%) < Brand C (101.46%). All of the brands met the BP and USP-NF specifications for assay. First-order, Higuchi and Korsmeyer–Peppas kinetics model fit for all of the mentioned ten brands.ConclusionThe present study revealed that all of the leading brands of this tablet met the quality control parameters as per pharmacopoeial specifications except dissolution test for four brands (Brand J, Brand H, Brand I, and Brand F).

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Section: Discussionmentioning

confidence: 99%

In vitro quality evaluation of leading brands of ciprofloxacin tablets available in Bangladesh

et al. 2017

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“…Spectrophotometric determination of degraded CIP was carried out by methyl orange method and Indigo carmine method (Basavaiah et al 2006;Nijhu et al 2011).…”

Section: Spectrophotometric Analysismentioning

confidence: 99%

Biodegradation of ciprofloxacin by white rot fungus Pleurotus ostreatus

2017

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Unrestricted and reckless use of antibiotics has resulted in their accumulation in environment. This, in turn, has led to the emergence of multiple drug-resistant microbes. The present study focuses on degradation of ciprofloxacin (CIP) by an edible white rot fungus Pleurotus ostreatus. Effect of CIP was determined on radial growth and biomass of P. ostreatus. Titrimetric and spectrophotometric assays were carried out to assess the degrading potential of P. ostreatus towards CIP. It was found that CIP has a stimulatory effect on growth and enzyme activity of P. ostreatus. Maximum enzyme (glucanase, ligninases, laccase) production was observed at the highest concentration of CIP (500 ppm). Antibiotic degradation of about 68.8, 94.25 and 91.34% was estimated after 14 days of incubation at 500 ppm CIP using Titrimetric, Indigo carmine and Methyl orange assay, respectively. Degradation of CIP was further validated by high performance liquid chromatography (HPLC) and microbiological analysis. HPLC analysis revealed 95.07% degradation while microbiological test also exhibited a decreased antimicrobial activity of degraded products against Escherichia coli, Staphylococcus aureus and Streptococcus pyogenes. To the best of our knowledge, this is the first study wherein P. ostreatus was used for the degradation of ciprofloxacin.

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“…The ciprofloxacin concentration in supernatants was determined by measuring the light absorption at a wavelength of 272 nm using a Nanodrop ND‐1000 UV‐Vis Spectrophotometer (Nanodrop Technologies, Wilmington). An OD 272 of 0.1 corresponded to 1 µg/mL ciprofloxacin …”

Section: Methodsmentioning

confidence: 99%

Controlled drug release from antibiotic‐loaded layered double hydroxide coatings on porous titanium implants in a mouse model

Badar

Rahim

Kieke

et al. 2014

J Biomedical Materials Res

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As an alternative to degradable organic coatings the possibility of using layered double hydroxides (LDHs) to generate implant coatings for controlled drug delivery was evaluated in vivo and in vitro. Coatings prepared from LDH suspensions dissolved slowly and appeared compatible with cultured cells. LDH coatings loaded with an antibiotic resulted in antibacterial effects in vitro. The LDH coating prolonged the drug release period and improved the proliferation of adherent cells in comparison to pure drug coatings. However, during incubation in physiological solutions the LDH coatings became brittle and pieces occasionally detached from the surface. For stress protection porous titanium implants were investigated as a substrate for the coatings. The pores prevented premature detachment of the coatings. To evaluate the coated porous implants in vivo a mouse model was established. To monitor bacterial infection of implants noninvasive in vivo imaging was used to monitor luminescently labeled Pseudomonas aeruginosa. In this model porous implants with antibiotic-loaded LDH coatings could antagonize bacterial infections for over 1 week. The findings provide evidence that delayed drug delivery from LDH coatings could be feasible in combination with structured implant surfaces.

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Development of an Assay Method for Simultaneous Determination of Ciprofloxacin and Naproxen by UV Spectrophotometric Method

Cited by 15 publications

References 9 publications

In vitro quality evaluation of leading brands of ciprofloxacin tablets available in Bangladesh

In vitro quality evaluation of leading brands of ciprofloxacin tablets available in Bangladesh

Biodegradation of ciprofloxacin by white rot fungus Pleurotus ostreatus

Controlled drug release from antibiotic‐loaded layered double hydroxide coatings on porous titanium implants in a mouse model

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