Development of a real-time multiplex PCR assay for the detection of multiple Salmonella serotypes in chicken samples

O'Regan, Edel; McCabe, Evonne; Burgess, Catherine M.; McGuinness, Sheila; Barry, Thomas; Duffy, Geraldine; Whyte, Paul; Fanning, Séamus

doi:10.1186/1471-2180-8-156

Cited by 100 publications

(70 citation statements)

References 37 publications

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“…However Salmonella Typhimurium, Infantis, Kottbus, Kentuky, Minnesota were detected as higher prevalence by (Voss Rech et al, 2015Muammer et al, 2016and Abde-Rahim, 2016 respectively. It has been reported that invA gene is present only in Salmonella species and therefore is used as a golden marker in genetic diagnosis of Salmonella species as previously described (O'Regan et al, 2008). In our study the invA gene was detected with 100% in all examined Salmonella serovars, high prevalence rates of invA virulence gene in Salmonella serovars has also been reported by other workers (Karmi, 2013 andChaudhary et al, 2015).…”

Section: Discussionsupporting

confidence: 87%

Molecular Detection of InvA, OmpA and Stn Genes in Salmonella Serovars from Broilers in Egypt

Fekry¹,

Eman-abdeen²,

Ammar³

et al. 2018

AJVS

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Key words:Salmonella, virulence genes, broiler, diarrheaThe present study was conducted to determinate the prevalence of some virulence genes among Salmonella serovars isolated from broiler flocks in Egypt. A total of 55 Salmonella isolates were recovered from different samples (liver, yolk sac, gall bladder and caecum) collected from apparently healthy and diseased broilers suffered from whitish diarrhea, dehydration and respiratory distress. Thirty isolates were serotyped into s. enteritidis (43.3%) s. infantis and s. Kentucky (16.6%), s.maloma and s.bardo (6.7%), s.gdansk, s.typhimurium and s. blegdame (3.3%). Molecular detections of invA, ompA and stn virulence genes in 15 Salmonella isolates were applied using specific primer sets. The results reported detection of the screening genes in all 15 examined isolates with 100%. In conclusion, these genes appear to have a critical role in pathogenicity of Salmonella infection in poultry. So, this help in understanding the process of pathogenicity and design of control and therapy treatment strategies for salmonellosis in broiler chickens.

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Section: Discussionsupporting

confidence: 87%

Molecular Detection of InvA, OmpA and Stn Genes in Salmonella Serovars from Broilers in Egypt

Fekry¹,

Eman-abdeen²,

Ammar³

et al. 2018

AJVS

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“…Table 2 shows the distribution of isolates and potential risk factors for the presence of Salmonella in chicken meat marketed at Ibague, Tolima. dISCuSSIon Salmonella spp., was isolated from 17.41% of chicken samples (47/270) marketed in Ibague, Tolima, and a number of serotypes were identified and confirmed by amplification of a fragment of the invA gene using PCR (Galan, Curtiss, 1991;Li et al, 2012;O'Regan et al, 2008;Shanmugasamy et al, 2011), a rapid and powerful technique used for Salmonella identification (Cardona-castro et al, 2007;Ibrahim et al, 2014;Molina et al, 2010;Tafida et al, 2013). Thus, this data is a representative estimation of the occurrence of Salmonella in a region with traditional poultry industry and it may indicate poor hygienic and disinfection practices, which have been associated with cross-contamination and recontamination of poultry carcasses (Carrasco et al 2012).…”

Section: Resultsmentioning

confidence: 99%

Serotypes of Salmonella in Broiler Carcasses Marketed at Ibague, Colombia.

Rodriguez

Rondón

Verján

2015

Rev. Bras. Cienc. Avic.

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Salmonella enterica is a large group of Gram-negative bacteria responsible for a number of foodborne infections associated with the consumption of contaminated poultry products. The hygienic status of raw chicken meat marketed at Ibague, Tolima, Colombia, is currently unknown. To address this issue, a cross-sectional study was conducted to estimate the prevalence of Salmonella spp., in raw chicken marketed at different outlets in this city. Salmonella spp. was isolated by standard microbiological methods, followed by biochemical, serological, and molecular confirmation. Additionally, risk factors associated with the presence of the bacteria were identified. The prevalence of Salmonella in raw chicken was 17.41% (47/270), and 14 different serotypes were found, out of which S. Paratyphi B (36.17%), S. Hvittingfoss (19.15%) and S. Muenster (10.64%) were the most prevalent and represented 65.95% of all serotypes. Amplification of 284 bp of the invA gene was achieved by PCR in a number of randomly selected isolates. Raw chicken as the only type of meat sold at stores (odds ratio: 2,157, p<0.05), and stainless steel as a contact surface of chicken meat (odds ratio: 13,29, p<0.05), were found to be potential risk factors for the presence of Salmonella in chicken meat. This work serves as a reference about the current status of Salmonella in chicken meat marketed in Ibague, Tolima, Colombia, and indicates the need to establish appropriate control and contingency measures to minimize the presence of the bacteria in raw chicken to prevent its transmission to humans.

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“…The supernatant was discarded, and the cell pellets were suspended in 1 ml phosphate buffered saline (PBS), centrifuged at 15,330 × g for 5 min. The supernatant was again discarded, and the pellets were suspended in 50 μl sterile distilled water for DNA extraction (O'Regan et al 2008). The suspension was heated at 99 ºC for 10 min (Shanmugasamy et al, 2011), and then pelleted by centrifugation at 2,656 × g for 5 min.…”

Section: Dna Extractionmentioning

confidence: 99%

Incidence and molecular detection of Salmonella enterica serogroups and spvC virulence gene in raw and processed meats from selected wet markets in Metro Manila, Philippines

Rosario¹,

Rivera²

2015

PhilSciTech

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Abstract-A simple and specific detection method for Salmonella enterica was applied to determine its incidence in raw and processed meats purchased from selected wet markets in Metro Manila, Philippines. A total of 320 raw and processed meat samples were analysed for the presence of S. enterica and S. enterica possessing spvC gene of the virulence plasmid. Polymerase chain reaction (PCR) revealed that 30.63% (98/320) were positive for S. enterica and 2.81% (9/98) of S. enterica-positive samples were also positive for the spvC gene. S. enterica was identified from chicken samples (67.5%), ground pork (65%), beef (52.5%), sausage (longganisa) (25%), cured pork meat (tocino) (20%), burger patty (12.5%), and meatloaf (embotido) (2.5%). Positive samples were further analysed for O-serogrouping targeting S. enterica serogroups A, B, C1, C2, D, and E1. Our findings revealed that the raw and processed meats tested were contaminated with more than one serogroup in a sample. Samples were found positive for S. enterica serogroups E1 (78.57%), C1 (29.59%), C2 (20.41%), B (17.35%) and D (6.1%). No samples were found positive for S. enterica serogroup A. This is the first report on the use of multiplex PCR for the detection and characterization of S. enterica in raw and processed meats in the Philippines. Data on incidence of S. enterica and its serogroups found in raw and processed meats in selected Philippine wet markets gathered from this study can be used for further research on epidemiology and related topics.

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Development of a real-time multiplex PCR assay for the detection of multiple Salmonella serotypes in chicken samples

Cited by 100 publications

References 37 publications

Molecular Detection of InvA, OmpA and Stn Genes in Salmonella Serovars from Broilers in Egypt

Molecular Detection of InvA, OmpA and Stn Genes in Salmonella Serovars from Broilers in Egypt

Serotypes of Salmonella in Broiler Carcasses Marketed at Ibague, Colombia.

Incidence and molecular detection of Salmonella enterica serogroups and spvC virulence gene in raw and processed meats from selected wet markets in Metro Manila, Philippines

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