Abstract-A simple and specific detection method for Salmonella enterica was applied to determine its incidence in raw and processed meats purchased from selected wet markets in Metro Manila, Philippines. A total of 320 raw and processed meat samples were analysed for the presence of S. enterica and S. enterica possessing spvC gene of the virulence plasmid. Polymerase chain reaction (PCR) revealed that 30.63% (98/320) were positive for S. enterica and 2.81% (9/98) of S. enterica-positive samples were also positive for the spvC gene. S. enterica was identified from chicken samples (67.5%), ground pork (65%), beef (52.5%), sausage (longganisa) (25%), cured pork meat (tocino) (20%), burger patty (12.5%), and meatloaf (embotido) (2.5%). Positive samples were further analysed for O-serogrouping targeting S. enterica serogroups A, B, C1, C2, D, and E1. Our findings revealed that the raw and processed meats tested were contaminated with more than one serogroup in a sample. Samples were found positive for S. enterica serogroups E1 (78.57%), C1 (29.59%), C2 (20.41%), B (17.35%) and D (6.1%). No samples were found positive for S. enterica serogroup A. This is the first report on the use of multiplex PCR for the detection and characterization of S. enterica in raw and processed meats in the Philippines. Data on incidence of S. enterica and its serogroups found in raw and processed meats in selected Philippine wet markets gathered from this study can be used for further research on epidemiology and related topics.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.