Development of a quantitative fluorescence-based lateral flow immunoassay for determination of chloramphenicol, thiamphenicol and florfenicol in milk

Abstract

A competitive lateral flow immunoassay (LFA) using fluorescent microspheres (FMs) as label was developed for the quantitative detection of chloramphenicol (CAP), thiamphenicol (TAP) and florfenicol (FF) in milk. The limit of detection (LOD) for CAP, TAP and FF in milk was 0.08, 0.8 and 1.9 μg L −1 , respectively. The recovery of intra and inter-assay ranged from 87.7% to 107.0% and 82.5% to 112.3%, with corresponding coefficient of variations less than 11.3% and 14.5%, respectively. The sensitivity of the FMs lateral flow assay (FMs-LFA) was comparable to those of the colloidal gold lateral flow assay. A quantitative comparison of the FMs-LFA and LC-MS/MS analysis of milk samples indicated good agreement between the two methods. The FMs-LFA can be used as a reliable, rapid and cost-effective method for food safety analysis. ARTICLE HISTORY

Development and comparison of liquid–liquid extraction and accelerated solvent extraction methods for quantitative analysis of chloramphenicol, thiamphenicol, florfenicol, and florfenicol amine in poultry eggs

“…To date, various methods have been used to measure CAP, TAP, FF, and FFA in animal-derived foods; these methods include the fluorescent microsphere lateral flow immunoassay (FM-LFA), 7 enzyme-linked immunosorbent assay (ELISA), 8,9 reversed-phase high-performance liquid chromatography with ultraviolet detection (RP-HPLC-UVD), 10 HPLC with diode array detection (DAD), 11 RP-HPLC with fluorescence detection (FLD), 12 LC-mass spectrometry (MS), 13,14 LC tandem mass spectrometry (MS/MS), [15][16][17][18][19][20] ultra-performance liquid chromatography (UPLC)-MS/ MS, [21][22][23][24] and gas chromatography-negative chemical ionization mass spectrometry (GC-NCI/MS). 25 In previous research, we developed an LC-MS/MS 20 method for the simultaneous determination of CAP, TAP, FF, and FFA in eggs.…”

Development of a multicomponent immunochromatographic test system for the detection of fluoroquinolone and amphenicol antibiotics in dairy products

“…Recently, several studies devoted to multicomponent ICAs for simultaneous detection of antibiotics, either from a single pharmacological group [29][30][31][32][33] or from different classes [30][31][32] have been proposed ( Table 1). In general, multicomponent ICAs seem to be a promising technology for effective and low-cost point-of-care screening of food products for relevant contaminants.…”

Development of an indirect competitive enzyme-linked immunosorbent assay for detecting flunixin and 5-hydroxyflunixin residues in bovine muscle and milk

“…high-performance liquid chromatography coupled with UV and mass spectrometry detectors (Asea, Patterson, Korsrud, Dowling, & Boison, 2001;Jedziniak, Szprengier-Juszkiewicz, & Olejnik, 2009;Liu et al, 2015;Peng et al, 2013;van Pamel & Daeseleire, 2015). Although these methods are very accurate and sensitive, they are costly and require complex sample pretreatment steps and trained personnel (Mukunzi, Suryoprabowo, Song, Liu, & Kuang, 2018;Qu et al, 2019;Wang et al, 2018;Zeng, Jiang, Liu, Song, & Kuang, 2018). Immunoassays are superior to chromatographic methods in screening tests for their simplicity, sensitivity, and high-throughput capabilities (Chen et al, 2017; Kong et al, 2017;Wang, Luo, Chen, Huang, & Jiang, 2016).…”