Development of an indirect competitive enzyme-linked immunosorbent assay for detecting flunixin and 5-hydroxyflunixin residues in bovine muscle and milk

Chen, Xiaona; Peng, Shimin; Liu, Chen; Zou, Xin; Ke, Yuebin; Jiang, Wenxiao

doi:10.1080/09540105.2019.1577365

Cited by 13 publications

(9 citation statements)

References 30 publications

(32 reference statements)

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“…The method validated as per commission decision 2002/657/EC detection capability (CC-β), linearity, decision limit CC-α, precision, and accuracy and were calculated using LC-MS/MS [25]. Chen et al [26] determined the flunixin residues in foods of animal origin which pose hazards for human health. They analyzed 5-hydroxyflunixin residue in milk and flunixin residue in bovine muscle by ELISA and validated by LC-MS/MS confirmatory method with coefficient variation ranging 5.8-11.3% and average spike recovery ranged 83-105%.…”

Section: Lc-msmentioning

confidence: 99%

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Assessment of various veterinary drug residues in animal originated food products

Parmar

Chaubey

Gupta³

et al. 2021

Vet World

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The veterinary drugs are broad-spectrum antibacterial antibiotics; it uses to cure the animal disease. Many countries have banned veterinary drug residues like nitrofurans metabolites, chloramphenicol. However, the people were administrated veterinary drugs to animals as illegal to increase the milk production in animals for economic benefit. The results of illegally use of veterinary drugs remain as a residue in animal product like milk and it is very harmful to whom consume it cause cancer and allergic for human being which has entered the concern among milk consumers. To control illegal use of veterinary drugs, the government of India has restricted its use in animals. For the identification and confirmation of veterinary drug residues in animal products, analytical techniques such as liquid chromatography and mass spectrometry are available. These are very sophisticated equipments which are available nowadays and their methodologies for the analytical method validation are described by European commission 2002/657/EC. The use of veterinary drugs is a big challenge to effectively identify and authorization of their use. There are so many analytical techniques are using very effectively and taking very less time to protect the consumers from their adverse effects. These techniques take very less time to identify more groups of compounds such as tetracycline, sulfonamides, anthelmintic, and macrolides in single multi-residue method. These methods having validation parameters include system precision, calibration curve, accuracy, limit of detection, and quantification. Therefore, improvement in the existing technologies and accessibility of new screening methodologies will give opportunities for automation that helps in obtaining the results in very less time and improved sensitivity and specificity which contribute to better safety assurance, standard, and quality of various food products of animal origin.

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Section: Lc-msmentioning

confidence: 99%

“…Chen et al . [ 26 ] determined the flunixin residues in foods of animal origin which pose hazards for human health. They analyzed 5-hydroxyflunixin residue in milk and flunixin residue in bovine muscle by ELISA and validated by LC-MS/MS confirmatory method with coefficient variation ranging 5.8-11.3% and average spike recovery ranged 83-105%.…”

Section: Lc-msmentioning

confidence: 99%

Assessment of various veterinary drug residues in animal originated food products

Parmar

Chaubey

Gupta³

et al. 2021

Vet World

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“…Lately, several antibodies for FLU and 5-FLU residue detection have been published (Table ). ,,, Brady et al coupled FLU to keyhole limpet hemocyanin (KLH) and produced polyclonal antibody (pAb) with a high IC 50 of 17.3 ng mL –1 . By using 5-FLU as a hapten coupled to bovine serum albumin (BSA) to prepare an immunogen, Fan et al obtained a monoclonal antibody (mAb) with an IC 50 of 3.2 ng mL –1 for 5-FLU and 3.3 ng mL –1 for FLU .…”

Section: Introductionmentioning

confidence: 99%

“…2 values from 0.996 to 0.999 for milk and muscle samples. Subsequently, 10 real milk samples, 10 real porcine muscle samples, 10 real bovine muscle samples, and 10 real sheep muscle samples from the market were simultaneously detected using established icELISA and HPLC-MS/MS.…”

mentioning

confidence: 99%

Antibody Production, Immunoassay Establishment, and Specificity Study for Flunixin and 5-Hydroxyflunixin

Li,

Mi,

Bai

et al. 2024

J. Agric. Food Chem.

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Flunixin (FLU) is a nonsteroidal drug that is widely used in animals, causing severe drug residues in animal-derived foods and environment. The development of antibody-based rapid immunoassay methods is of great significance for the monitoring of FLU and its metabolite 5-hydroxyflunixin (5-FLU). We prepared monoclonal antibodies (mAbs) with different recognition spectra through FLU-keyhole limpet hemocyanin conjugates as immunogen coupled with antibody screening strategies. mAb5E6 and mAb6D7 recognized FLU with high affinity, and mAb2H5 and mAb4A4 recognized FLU and 5-FLU with broad specificity. Through evaluating the recognition of these mAbs against more than 11 structural analogues and employing computational chemistry, molecular docking, and molecular dynamics methodologies, we preliminarily determined the recognition epitope and recognition mechanism of these mAbs. Finally, an indirect competitive enzyme-linked immunosorbent assay for FLU based on mAb6D7 was developed, which exhibited limits of detection as low as 0.016−0.042 μg kg −1 (L −1 ) in milk and muscle samples.

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“…On the other hand, some of their biodegraded products have the same even enhanced toxicity compared with those of their parent pesticides (Rahemi, Garrido, Borges, Brett, & Garrido, 2015). To ensure food and environmental safety, simultaneously rapid detection of pesticides and their toxic metabolites in the real samples is required (Chen et al, 2019;Hao, Suryoprabowo, Song, Liu, & Kuang, 2018). Carbofuran (CBF), a broad-spectrum carbamate pesticide, has been applied for pest control in a variety of crops worldwide for decades.…”

mentioning

confidence: 99%

Simultaneous and rapid detection of carbofuran and 3-hydroxy-carbofuran in water samples and pesticide preparations using lateral-flow immunochromatographic assay

Lan

Sun

Chen³

et al. 2020

Food and Agricultural Immunology

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It is long-cherished to develop rapid approaches for the identification and quantification of pesticide and its metabolites. Carbofuran (CBF) and its metabolite 3-hydroxy-carbofuran (3-OH-CBF) are highly toxic to humans and non-target organisms, which makes it mandatory for the detection of their residues in foods and the environment in many countries. Herein, a colloidal goldstrip assay based on a broad-specific monoclonal antibody (mAb) for simultaneous determination of CBF and 3-OH-CBF was developed. The colloidal gold-strips have a runtime within 5 min without complex sample pretreatment and perform a cut-off limit of detection (LOD) of 7-10 ng/mL for both carbofuran and 3-OH-CBF. The developed portable strip assay is a versatile and robust tool for the rapid and simultaneous detection of CBF and 3-OH-CBF in water samples as well as for the determination of illegal addition of CBF in other pesticide preparations.

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Development of an indirect competitive enzyme-linked immunosorbent assay for detecting flunixin and 5-hydroxyflunixin residues in bovine muscle and milk

Cited by 13 publications

References 30 publications

Assessment of various veterinary drug residues in animal originated food products

Assessment of various veterinary drug residues in animal originated food products

Antibody Production, Immunoassay Establishment, and Specificity Study for Flunixin and 5-Hydroxyflunixin

Simultaneous and rapid detection of carbofuran and 3-hydroxy-carbofuran in water samples and pesticide preparations using lateral-flow immunochromatographic assay

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