Simultaneous and rapid detection of carbofuran and 3-hydroxy-carbofuran in water samples and pesticide preparations using lateral-flow immunochromatographic assay

Abstract

It is long-cherished to develop rapid approaches for the identification and quantification of pesticide and its metabolites. Carbofuran (CBF) and its metabolite 3-hydroxy-carbofuran (3-OH-CBF) are highly toxic to humans and non-target organisms, which makes it mandatory for the detection of their residues in foods and the environment in many countries. Herein, a colloidal goldstrip assay based on a broad-specific monoclonal antibody (mAb) for simultaneous determination of CBF and 3-OH-CBF was developed. The colloidal gold-strips have a runtime within 5 min without complex sample pretreatment and perform a cut-off limit of detection (LOD) of 7-10 ng/mL for both carbofuran and 3-OH-CBF. The developed portable strip assay is a versatile and robust tool for the rapid and simultaneous detection of CBF and 3-OH-CBF in water samples as well as for the determination of illegal addition of CBF in other pesticide preparations.

An Overview for the Nanoparticles‐Based Quantitative Lateral Flow Assay

“…The color changes significantly when a large numbers of AuNPs aggregate together, which is the basis for their application to colorimetric immunoassays. [ 48 ] Various approaches are used to link detection biomolecules to AuNP surfaces, including physical adsorption and covalent binding. The physical adsorption method is simple, whereas the covalent binding method generates a much stronger bond between the AuNP and the biomolecule, which generally contains sulfhydryl or amino functional groups.…”

Point-of-care diagnostics for infectious diseases: From methods to devices

“…Lateral flow devices are one of the most widely used POC diagnostic tools for the detection of infectious diseases owing to their advantages of simplicity, easy operation, rapidness, durable stability, cost-effectiveness, and minimum user intervention [412] , [413] , [414] . Since the first lateral flow device developed in the 1980s for pregnancy self-testing, there have been various lateral flow devices developed for all kinds of applications, including biomarker detection, disease diagnosis, food safety, environment monitoring and so on [415] , [416] , [417] , [418] , [419] , [420] . A typical lateral flow device consists of a nitrocellulose membrane, sample pad, conjugate pad, and absorbent pad with all the required chemicals and reagents pre-stored in the test strip [393] , [421] .…”

Optical Screening Methods for Pesticide Residue Detection in Food Matrices: Advances and Emerging Analytical Trends

“…In terms of big molecules, for example, allergens, the sandwich format is used, and the analyte is immobilized between two complementary antibodies. Besides research studies using LF assays for pesticide residue screening [ 54 , 55 ], LF assays are one of the few cases that have reached the commercialization stage [ 19 ]. Regarding microfluidics, this is a relatively new field that was established in 2006 following the publication of G.M Whitesides in the prestigious Nature journal [ 56 ].…”