Point-of-care diagnostics for infectious diseases: From methods to devices

Wang, Chao; Liu, Mei; Wang, Zhifei; Li, Song; Yan, Dawei; He, Nongyue

doi:10.1016/j.nantod.2021.101092

Cited by 335 publications

(235 citation statements)

References 533 publications

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“…Although these detection methods have good performance, they still have many shortcomings [203]. For electrochemical detection methods, they have high sensitivity, fast response, and low cost, but stability and susceptibility to interference are weak [204].…”

Section: Magnetic Detectionmentioning

confidence: 99%

Public-Health-Driven Microfluidic Technologies: From Separation to Detection

Zhang

Wang

et al. 2021

Micromachines

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Separation and detection are ubiquitous in our daily life and they are two of the most important steps toward practical biomedical diagnostics and industrial applications. A deep understanding of working principles and examples of separation and detection enables a plethora of applications from blood test and air/water quality monitoring to food safety and biosecurity; none of which are irrelevant to public health. Microfluidics can separate and detect various particles/aerosols as well as cells/viruses in a cost-effective and easy-to-operate manner. There are a number of papers reviewing microfluidic separation and detection, but to the best of our knowledge, the two topics are normally reviewed separately. In fact, these two themes are closely related with each other from the perspectives of public health: understanding separation or sorting technique will lead to the development of new detection methods, thereby providing new paths to guide the separation routes. Therefore, the purpose of this review paper is two-fold: reporting the latest developments in the application of microfluidics for separation and outlining the emerging research in microfluidic detection. The dominating microfluidics-based passive separation methods and detection methods are discussed, along with the future perspectives and challenges being discussed. Our work inspires novel development of separation and detection methods for the benefits of public health.

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Section: Magnetic Detectionmentioning

confidence: 99%

Public-Health-Driven Microfluidic Technologies: From Separation to Detection

Zhang

Wang

et al. 2021

Micromachines

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“…Additional confirmatory tests include ELISA, PCR, DNA chips and chromatographic techniques, which require sophisticated instruments, professional operators, and costly chemicals. More recently, newer POC biosensors have emerged as newer diagnostic tools but many of them suffer from low sensitivity, specificity and reliability ( Sheikhzadeh et al, 2021 , Wang et al, 2021 ). Biosensors are rapidly becoming a cutting-edge novel technology in disease diagnosis and treatment.…”

Section: Introductionmentioning

confidence: 99%

Aptamer-based diagnostic and therapeutic approaches in animals: Current potential and challenges

Devi

Sharma

Ahmed

et al. 2021

Saudi Journal of Biological Sciences

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Fast and precise diagnosis of infectious and non-infectious animal diseases and their targeted treatments are of utmost importance for their clinical management. The existing biochemical, serological and molecular methods of disease diagnosis need improvement in their specificity, sensitivity and cost and, are generally not amenable for being used as points-of-care (POC) device. Further, with dramatic changes in environment and farm management practices, one should also arm ourselves and prepare for emerging and re-emerging animal diseases such as cancer, prion diseases, COVID-19, influenza etc. Aptamer – oligonucleotide or short peptides that can specifically bind to target molecules – have increasingly become popular in developing biosensors for sensitive detection of analytes, pathogens (bacteria, virus, fungus, prions), drug residues, toxins and, cancerous cells. They have also been proven successful in the cellular delivery of drugs and targeted therapy of infectious diseases and physiological disorders. However, the in vivo application of aptamer-mediated biosensing and therapy in animals has been limited. This paper reviews the existing reports on the application of aptamer-based biosensors and targeted therapy in animals. It also dissects the various modifications to aptamers that were found to be successful in in vivo application of the aptamers in diagnostics and therapeutics. Finally, it also highlights major challenges and future directions in the application of aptamers in the field of veterinary medicine.

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“…Due to their simplicity and the ability to be easily modified and applied to different fields, aptamerbased SPR biosensors have been used to detect DNA [18], protein [19,20], nanoparticle [21], small molecules [22] and microRNA [23]. Compared with the conventional methods and other sensing strategies, this aptamer-based SPR biosensor system has several advantages such as faster, simpler, lower-cost, more practical, and better stability [24]. The proposed aptamer-based SPR biosensor could detect HIV-related DNA sensitively and specifically with a wide linear range from 1 pM to 150 nM and a detection limit of 48 fM, which is promising for the point-of-care detection of HIV infections [25].…”

Section: Introductionmentioning

confidence: 99%

Biosensing Amplification by Hybridization Chain Reaction on Phase-Sensitive Surface Plasmon Resonance

Yang

Chang

et al. 2021

Biosensors

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Surface Plasmon Resonance (SPR) is widely used in biological and chemical sensing with fascinating properties. However, the application of SPR to detect trace targets is hampered by non-specific binding and poor signal. A variety of approaches for amplification have been explored to overcome this deficiency including DNA aptamers as versatile target detection tools. Hybridization chain reaction (HCR) is a high-efficiency enzyme-free DNA amplification method operated at room temperature, in which two stable species of DNA hairpins coexist in solution until the introduction of the initiator strand triggers a cascade of hybridization events. At an optimal salt condition, as the concentrations of H1 and H2 increased, the HCR signals were enhanced, leading to signal amplification reaching up to 6.5-fold of the detection measure at 30 min. This feature enables DNA to act as an amplifying transducer for biosensing applications to provide an enzyme-free alternative that can easily detect complex DNA sequences. Improvement of more diverse recognition events can be achieved by integrating HCR with a phase-sensitive SPR (pSPR)-tested aptamer stimulus. This work seeks to establish pSPR aptamer system for highly informative sensing by means of an amplification HCR. Thus, combining pSPR and HCR technologies provide an expandable platform for sensitive biosensing.

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Point-of-care diagnostics for infectious diseases: From methods to devices

Abstract: Graphical Abstract Point-of-care diagnostics for infectious diseases.

Cited by 335 publications

References 533 publications

Public-Health-Driven Microfluidic Technologies: From Separation to Detection

Public-Health-Driven Microfluidic Technologies: From Separation to Detection

Aptamer-based diagnostic and therapeutic approaches in animals: Current potential and challenges

Biosensing Amplification by Hybridization Chain Reaction on Phase-Sensitive Surface Plasmon Resonance

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