An Overview for the Nanoparticles‐Based Quantitative Lateral Flow Assay

Wang, Zhongxing; Zhao, Jing; Xu, Xinxin; Guo, Lingling; Xu, Liguang; Sun, Maozhong; Hu, Shudong; Kuang, Hua; Li, Aike

doi:10.1002/smtd.202101143

Cited by 55 publications

(39 citation statements)

References 263 publications

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“…sGNPs were synthesized by the citrate technique and characterized by transmission electron microscopy—see Figure 2 . The average diameter of the obtained nanoparticles was 32 ± 7 nm, which accords to generally accepted recommendations about the best size of GNPs for ICA [ 33 ].…”

Section: Resultssupporting

confidence: 80%

Triple Enhancement for Sensitive Immunochromatographic Assay: A Case Study for Human Fatty Acid-Binding Protein Detection

et al. 2022

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The work considers a combination of three enhancing approaches for immunochromatographic assay (ICA) and the integration of their impacts into changes of the limit of detection (LOD). Human fatty acid binding protein (FABP), an early biomarker of acute myocardial infarction, was the target analyte. Starting from the common ICA protocol with an LOD equal to 11.2 ng/mL, three approaches were realized: (1) replacement of spherical gold nanoparticles with gold nanoflowers having a branched surface (20-fold lowering the LOD); (2) enhanced labeling of immune complexes via nanoparticle aggregates (15-fold lowering); (3) in-situ growth of bound nanoparticles by reduction of gold salts (3-fold lowering). Single and combined implementations of these approaches have been studied. It has been shown that the LOD decrease for combined approaches is close to the multiplied contribution of each of them. The final LOD for FABP was 0.05 ng/mL, which is 220 times lower than the LOD for the common ICA protocol. The efficiency of the enhanced ICA with three combined approaches was confirmed by testing human serum samples for FABP presence and content. The development presents a new efficient technique for rapid sensitive detection of FABP for medical diagnostics. Moreover, the demonstrated multiple enhancements could be applied for various demanded analytes.

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Section: Resultssupporting

confidence: 80%

Triple Enhancement for Sensitive Immunochromatographic Assay: A Case Study for Human Fatty Acid-Binding Protein Detection

et al. 2022

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“…Lateral flow immunochromatographic assay test strips are composed of a conjugation pad (7 mm × 300 mm), absorbent pad (16 mm × 300 mm), sample pad (17 mm × 300 mm), and nitrocellulose membrane (NC membrane) were assembled on a plastic adhesive backing layer ( 41 ). The solution containing the target analyte is dropped on the sample pad.…”

Section: Methodsmentioning

confidence: 99%

A highly sensitive lateral flow immunoassay for the rapid and on-site detection of enrofloxacin in milk

et al. 2022

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Enrofloxacin (ENR) is a veterinary antibiotic used to treat bacterial infections in livestock. It chiefly persists in foods and dairy products, which in turn pose severe risks to human health. Hence it is very important to detect the ENR in foods and dairy products to safeguard human health. Herein, we attempted to develop a single-step detection lateral flow immunochromatographic assay (LFIA) using gold nanoparticles (AuNPs) for the rapid and on-site detection of ENR in milk samples. An anti-enrofloxacin monoclonal antibody (ENR-Ab) was conjugated with AuNPs for the specific detection of ENR in milk samples. For sensitivity improvement, many optimization steps were conducted on LFIA test strips. The visual limit of detection (vLOD) was found to be 20 ng/ml with a cut-off value of 50 ng/ml in the milk samples. The obtained LOD and cut-off value were within the safety limit guidelines of the Ministry of food and drug safety, South Korea. The test strip showed negligible cross-reactivity with ENR analogs, and other components of antibiotics, this indicates the high specificity of the LFIA test strip towards ENR. The designed test strip showed good reliability. The visual test results can be seen within 10 min without the need for special equipment. Therefore, the test strip can be employed as a potential detection strategy for the qualitative on-site detection of enrofloxacin in milk samples.

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“…Various reagents can help visualize antigen–antibody interactions, but gold nanoparticles (AuNPs) are most frequently used in test strips. When many AuNPs aggregate, their color changes notably, and their application in colorimetric immunoassays is based on this principle [ 43 ]. AuNP-based LFAs have been used to detect β-adrenergic agonists.…”

Section: Applications For Detecting β-Adrenergic Agonist Residuesmentioning

confidence: 99%

“…Furthermore, background absorption can be minimized as there is anti-Stokes luminescence; no photo-degradation of biomolecules occurs due to excitation in the infrared region [ 71 ]. Therefore, when compared with fluorescent organic dyes and QDs, UCNPs have several advantages, such as higher photo-stability, a longer fluorescence lifespan, lower cost, and lower cytotoxicity [ 43 ]. Wang et al [ 47 ] adopted a UCNP-based LFA device for the sensitive detection of ultra-trace CLE in animal urine, tissues, and feed samples.…”

Section: Novel Labels For Use In Lfas To Detect β-Adrenergic Agonist ...mentioning

confidence: 99%

Paper-Based Fluidic Sensing Platforms for β-Adrenergic Agonist Residue Point-of-Care Testing

et al. 2022

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The illegal use of β-adrenergic agonists during livestock growth poses a threat to public health; the long-term intake of this medication can cause serious physiological side effects and even death. Therefore, rapid detection methods for β-adrenergic agonist residues on-site are required. Traditional detection methods such as liquid chromatography have limitations in terms of expensive instruments and complex operations. In contrast, paper methods are low cost, ubiquitous, and portable, which has led to them becoming the preferred detection method in recent years. Various paper-based fluidic devices have been developed to detect β-adrenergic agonist residues, including lateral flow immunoassays (LFAs) and microfluidic paper-based analytical devices (μPADs). In this review, the application of LFAs for the detection of β-agonists is summarized comprehensively, focusing on the latest advances in novel labeling and detection strategies. The use of μPADs as an analytical platform has attracted interest over the past decade due to their unique advantages and application for detecting β-adrenergic agonists, which are introduced here. Vertical flow immunoassays are also discussed for their shorter assay time and stronger multiplexing capabilities compared with LFAs. Furthermore, the development direction and prospects for the commercialization of paper-based devices are considered, shedding light on the development of point-of-care testing devices for β-adrenergic agonist residue detection.

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An Overview for the Nanoparticles‐Based Quantitative Lateral Flow Assay

Cited by 55 publications

References 263 publications

Triple Enhancement for Sensitive Immunochromatographic Assay: A Case Study for Human Fatty Acid-Binding Protein Detection

Triple Enhancement for Sensitive Immunochromatographic Assay: A Case Study for Human Fatty Acid-Binding Protein Detection

A highly sensitive lateral flow immunoassay for the rapid and on-site detection of enrofloxacin in milk

Paper-Based Fluidic Sensing Platforms for β-Adrenergic Agonist Residue Point-of-Care Testing

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