Development and validation of RP-HPLC-UV method for the determination of Glipizide in human plasma

Atif, Muhammad; Khalid, Syed Haroon; Kit, G.L. Onn; Sulaiman, Siti Amrah; Asif, Muhammad; Chandersekaran, A.

doi:10.1016/j.jyp.2013.01.005

Cited by 9 publications

(5 citation statements)

References 7 publications

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“…The parameters of optimum drug resolution value of endogenous biologic substances, best peak shape, and reasonable retention time were used to determine the composition of the mobile phase. Validation of the percentage differentiation parameter of ≤20% revealed that the developed analytical method acquired a LLOQ of 25 ng/mL, which is comparable with the values in earlier investigations at a concentration of 50 ng/mL [ 46 ]. Therefore, the proposed bioanalytic approach can determine analyte concentrations in plasma with accuracy and precision at the LLOQ [ 47 ].…”

Section: Discussionsupporting

confidence: 78%

Pharmacokinetic Herb-Drug Interactions of Glipizide with Andrographis paniculata (Burm. f.) and Andrographolide in Normal and Diabetic Rats by Validated HPLC Method

et al. 2022

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Co-administered medicinal herbs can modify a drug’s pharmacokinetics (PK), effectiveness, and toxicity. Andrographis paniculata (Burm. f.) ethanolic extract (APE) and andrographolide (AND) (a potent CYP2C9 inducer/inhibitor) can alter the pharmacokinetic parameters of glipizide (GLZ). This study aimed to determine the potential pharmacokinetics of herb–drug interactions between GLZ and APE/AND in the plasma of normal and diabetic rats using the HPLC bioanalysis method. The glipizide bioanalytical method established with RP-HPLC/UV instrument was validated following the EMA guidelines. GLZ was administered alone and in combination with APE or AND to normal and diabetic rats. The GLZ pharmacokinetic parameters were estimated according to the correlation between concentration and sampling time using the PK solver program. A simple and rapid GLZ bioanalysis technique with a lower limit of quantitation of 25 ng/mL was developed and presented the following parameters: accuracy (error ≤ 15%), precision (CV ≤ 15%), selectivity, stability, and linearity (R2 = 0.998) at concentrations ranging 25–1500 ng/mL. APE administration significantly improved the Cmax and AUC0–t/AUC0–∞ GLZ values in normal and diabetic rats (p < 0.05). AND significantly reduced the bioavailability of GLZ in diabetic rats with small values of T 1/2, Cmax, and AUC0–t/AUC0–∞ (p < 0.05). This combination can be considered in administering medications because it can influence the pharmacological effects of GLZ.

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Section: Discussionsupporting

confidence: 78%

Pharmacokinetic Herb-Drug Interactions of Glipizide with Andrographis paniculata (Burm. f.) and Andrographolide in Normal and Diabetic Rats by Validated HPLC Method

et al. 2022

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“…The injection volume capacity was 10 μL. The separation of GLZ was executed using “Acquity UPLC BEH C18 column (2.1 × 50 mm 2 , 1.7 μm, Waters)” maintained at T = 303.2 ± 1 K. The proposed UPLC-UV technique was used to quantify GLZ with some modifications in the reported HPLC-UV methods. − In the proposed analytical methodology, the mobile phase (composed of 80:20, v/v methanol and 10 mM KH 2 PO 4 buffer of pH 4.2 adjusted with orthophosphoric acid) was flowed with an isocratic mode. The flow rate and injection volume were set at 0.08 mL min –1 and 5 μL, respectively.…”

Section: Methodsmentioning

confidence: 99%

Solubility Measurement and Various Solubility Parameters of Glipizide in Different Neat Solvents

et al. 2020

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Glipizide (GLZ) is an oral hypoglycemic agent, which is a weakly aqueous soluble drug. The solubility values of GLZ in various neat solvents are scarce in the literature. Hence, the solubility of GLZ in 12 different neat solvents, namely, "water, methanol, ethanol, isopropanol (IPA), 1-butanol, 2-butanol, ethylene glycol (EG), propylene glycol (PG), poly(ethylene glycol)-400 (PEG-400), ethyl acetate (EA), dimethyl sulfoxide (DMSO), and Transcutol-HP (THP)", at "T = 298.2−318.2 K" and "p = 0.1 MPa" was measured. The recorded solubilities of GLZ were correlated by "van't Hoff and Apelblat models" using rootmean-square deviation (RMSD). The overall RMSD was obtained as 1.21 and 1.40% for "Apelblat and van't Hoff models", respectively. Different solubility parameters of all studied materials including drug and solvent were calculated to find the best solvent for GLZ. The solubilities of GLZ (expressed in mole fraction) have been found highest in DMSO (2.81 × 10 −2 ), followed by THP, EA, 2-butanol, 1-butanol, IPA, PEG-400, ethanol, PG, methanol, EG, and water (1.98 × 10 −4 ) at "T = 318.2 K". All investigated solubility parameters of GLZ were recorded very close to the DMSO. "Apparent thermodynamic analysis" showed an "endothermic and entropy-driven dissolution" of GLZ in the 12 different neat solvents. The highest molecular interactions were recorded in GLZ−DMSO compared to other combinations. Overall, DMSO has been considered as the best solvent for the solubilization of GLZ.

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“…The assay precision was carried out by interday and intraday study and the process was evaluated for solutions (20, 40, 60 µg/ml) and were analyzed at different time points. The % RSD (Relative standard deviation) was calculated for the methods 10 .…”

Section: Precision and Accuracymentioning

confidence: 99%

HPLC Method Development and Validation for Determination of Glibenclamide in Pharmaceutical Dosage Forms

Ashwini¹,

Sogali²,

Bhattacharyya³

2019

Int. Res. J. Pharm.

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Glibenclamide is an oral hypoglycemic agent, which increases the insulin secretion by binding to the sulfonylurea receptors on the beta cells or with ATP sensitive potassium channels on the pancreatic beta cells. The present study includes development of HPLC technique for determination and estimation of glibenclamide in pharmaceutical dosage forms using acetonitrile: phosphate buffer (70:30) as mobile Phase. The detection wavelength of glibenclamide was found to be at 235 nm. The developed method was validated for its linearity, precision, accuracy, specificity, robustness and determination of limit of quantification and limit of detection in the mobile phase. The linearity demonstrated a correlation coefficient of 0.999. Thus, this method can be considered to be precise, reliable, rapid, simple, sensitive and cost effective for determination of glibenclamide in pharmaceutical formulations.

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Development and validation of RP-HPLC-UV method for the determination of Glipizide in human plasma

Cited by 9 publications

References 7 publications

Pharmacokinetic Herb-Drug Interactions of Glipizide with Andrographis paniculata (Burm. f.) and Andrographolide in Normal and Diabetic Rats by Validated HPLC Method

Pharmacokinetic Herb-Drug Interactions of Glipizide with Andrographis paniculata (Burm. f.) and Andrographolide in Normal and Diabetic Rats by Validated HPLC Method

Solubility Measurement and Various Solubility Parameters of Glipizide in Different Neat Solvents

HPLC Method Development and Validation for Determination of Glibenclamide in Pharmaceutical Dosage Forms

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