Development and Validation of a Green Rp-HPLC Method for Quantification of Cefadroxil Capsules

Marco, Bianca Aparecida de

doi:10.20959/wjpps20178-9875

Cited by 9 publications

(9 citation statements)

References 12 publications

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“…The accuracy of the method was performed through a comparative analysis of the methods [12], in which the results obtained in this validation were compared with a second physicochemical method (High Performance Liquid Chromatography -HPLC) already validated for the drug, in which it presents similar concentrations worked on the linear curve [14]. The comparative assay was performed using cefadroxil sample solution.…”

Section: Accuracymentioning

confidence: 99%

New, Green and Miniaturized Analytical Method for Determination of Cefadroxil Monohydrate in Capsules

Marco¹,

Kogawa²,

Salgado³

2019

Drug Anal. Res.

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Cefadroxil, an oral antimicrobial, presents few techniques optimized for the reduction of solvents and toxic residues and/or non-use of them. So, a quantitative, new and miniaturized method for determination of cefadroxil monohydrate in capsules has been developed and validated by spectrophotometric method in the visible region according to the international guidelines. The analyzes were performed using microplates containing 96 wells, 1 % of phenolphthalein and sodium hydroxide 0.1 M as reagent at 552 nm. The method was (i) linear in the range of 15-115 µg mL-1, (ii) selective when comparing standard, sample, adjuvants and color reagent, (iii) precise with deviations below 4 %, (iv) accurate when comparing the proposed method with the HPLC method, (v) robusts by making small and deliberate modifications to the method, (vi) besides being fast, low cost, eco-friendly and generates minimal amount of waste. The method can be applied to the routine quality control of cefadroxil monohydrate in capsules and an effective and accessible alternative that contemplates the concepts of current and sustainable green analytical chemistry.

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Section: Accuracymentioning

confidence: 99%

New, Green and Miniaturized Analytical Method for Determination of Cefadroxil Monohydrate in Capsules

Marco¹,

Kogawa²,

Salgado³

2019

Drug Anal. Res.

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“…Many methods use buffer solutions that are non-toxic to the environment and the operator, but can shorten the life of the equipment and accessories, for example, the chromatographic columns, impacting the cost of the analysis. [60][61] It is necessary to develop methods that use solvents with low toxicity (ethanol and water, for example), as well as to use them in low concentrations. The commitment to using reduced samples through miniaturization, decreasing process steps and pretreatment of samples, are alternatives that directly influence the amount of reagents used, time of analysis, number of materials required and cost involved.…”

Section: And 324 Nmmentioning

confidence: 99%

A Critical Review of Analytical Methods for Determination of Ceftriaxone Sodium

Trindade

Salgado

2018

Critical Reviews in Analytical Chemistry

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Ceftriaxone sodium is a third-generation semi-synthetic antibiotic belonging to the class of cephalosporins. Is administered only by parenteral route and has the ability to cross the blood-brain barrier. It has bactericidal action; its main activity is related to the Gram-negative bacteria, being also able to act against Gram-negative bacilli resistant to the first- and second-generation cephalosporins. The present study presents a survey of the characteristics, properties and analytical methods used for the determination of ceftriaxone sodium, for the gathering of data searches were carried out in scientific articles in the world literature, as well as in the official compendia. It is necessary to create awareness about the importance of developing effective and reliable analytical methods for quality control and consequently for conducting pharmacokinetic, bioavailability, bioequivalence studies as well as for the therapeutic monitoring of this drug. Most of the methods found use high-performance liquid chromatography, but also methods that use absorption spectroscopy ultraviolet, infrared spectroscopy, spectrofluorimetry and microbiological methods have been presented. A discussion was presented highlighting the need to develop new ecological methods using less toxic solvents, rapid analysis and miniaturization of the samples.

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“…In this method, 8 assays are performed. The assays are performed separately to determine the effects of variation of 7 different steps (19,21,28,(35)(36)(37). Table 2 shows the variations performed.…”

Section: Robustnessmentioning

confidence: 99%

“…Nowadays, green analytical methods are relevant and advantageous to industries because it brings less environmental impacts, low analytical costs and higher operator safety. That is why there are many validated physical-chemical and microbiological methods for drugs quantification that apply the concept of green analytical chemistry to help in the environmental preservation, following the same line of this work in cases of HLPC methods that use ethanol and water in the mobile phase (14)(15)(16)(17)(18)(19)(20)(21). In the case of HPLC analysis, the use of ethanol instead of solvents such as methanol and acetonitrile reduces the toxicity to the analyst and generates less waste to the environment.…”

Section: Introductionmentioning

confidence: 99%

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Green Analytical Method for Quantification of Secnidazole in Tablets by HPLC-Uv

Lima¹,

Kogawa²,

Salgado³

2018

Drug Anal. Res.

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A simple, rapid, economic and green analytical method was validated for the determination of secnidazole in tablets. The aim was to contribute to the green analytical chemistry since it has low use of organic solvent and low production of toxic waste. For the HPLC-UV method, the mobile phase was a mixture of purified water + 0.7 % acetic acid and ethanol (78:22, v/v), ﬂow rate was 1.3 mL min-1 on column CN Phenomenex Luna (250 x 4.60 mm, 5 μm particle size), injection volume was 20 μL with UV detection at 318 nm and retention time of 4.26 minutes. The method was linear over the concentration range of 5-100 μg mL-1 (r = 0.9998) with limits of detection and quantitation of 0.533 e 1.615 μg mL-1, respectively. The precision of the method showed RSD less than 2 %. The accuracy determined by the average recoveries was 99.58 %. The secnidazole tablets were subjected to oxidation, acid, alkaline, neutral and photolysis degradation as stress conditions and the method was considered as indicative of stability. The method is adequate and safe to be a great alternative method in routine quality control analyzes for determination and quantification of secnidazole tablets.

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Development and Validation of a Green Rp-HPLC Method for Quantification of Cefadroxil Capsules

Cited by 9 publications

References 12 publications

New, Green and Miniaturized Analytical Method for Determination of Cefadroxil Monohydrate in Capsules

New, Green and Miniaturized Analytical Method for Determination of Cefadroxil Monohydrate in Capsules

A Critical Review of Analytical Methods for Determination of Ceftriaxone Sodium

Green Analytical Method for Quantification of Secnidazole in Tablets by HPLC-Uv

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