Development and Evaluation of Insulin Incorporated Nanoparticles for Oral Administration

Prusty, Amiya Kumar; Sahu, Susanta Kumar

doi:10.1155/2013/591751

Cited by 32 publications

(14 citation statements)

References 14 publications

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“…Area under the curves (AUC) determined from the concentration versus time plot using the linear trapezoidal method. Relative bio availabilities were calculated by the following formula using the AUC of orally fed insulin solution, insulin‐loaded core–shell nano‐particles and subcutaneous (SC) injection of insulin along with their respective doses

Relative bioavailability (RA) = \frac{AUC ​ (Oral) \times Dose (Sc)}{AUC (Sc) ​ \times Dose (Oral)} \times 100

…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Polyurethane‐incorporated chitosan/alginate core–shell nano‐particles for controlled oral insulin delivery

Bhattacharyya

Nasim

Mishra

et al. 2018

J of Applied Polymer Sci

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Chitosan (CS) and polyurethane-chitosan (PU-CS) nano-particles (NPs) were prepared for the core formation by complex coacervation method whereas alginate (ALG) and PU-ALG were crosslinked by ionic gelation method to form the protective shelllayer over the core. Effects of PU incorporation either within the core or shell or both were investigated by different in vitro and in vivo parameters. Fourier transform infrared (FTIR) spectroscopy of different compositions of nano-particles showed distinct characteristic peaks for CS, PU, and ALG, indicating their presence in variable ratios. Significance of polyurethane-incorporated systems towards insulin encapsulation efficiency, swelling parameters, insulin release, and in vivo pharmacological effect were also studied. Particle sizes, zeta potential, morphological analysis, mucoadhesion study, and in vivo acute toxicity studies of these core-shell nanoparticles were also performed. Bioavailability of insulin ranged from 9.04% to 11.6% for polyurethane-incorporated chitosan-alginate core-shell nano-particle formulations which was significantly higher than the insulin bioavailability of basic CS/ALG core-shell nanoparticle system.

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Relative bioavailability (RA) = \frac{AUC ​ (Oral) \times Dose (Sc)}{AUC (Sc) ​ \times Dose (Oral)} \times 100

…”

Section: Methodsmentioning

confidence: 99%

“…Insulin is one of the essential drugs which was massively explored to deliver through oral route in the recent past . Insulin is a protein, composed of two polypeptide chains of 21 and 30 amino acid residues joined by two disulfide bridges .…”

Section: Introductionmentioning

confidence: 99%

Polyurethane‐incorporated chitosan/alginate core–shell nano‐particles for controlled oral insulin delivery

Bhattacharyya

Nasim

Mishra

et al. 2018

J of Applied Polymer Sci

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“…For mucoadhesion analysis of chitosan-coated NPs versus uncoated PLGA NPs, energetic and geometric stability of the polymer-mucin molecular complexes were determined using static lattice atomistic simulations (molecular mechanics simulations; Chemlite30, Hypercube Inc., Gainesville, FL, USA). The structures of PLGA and PEG were generated as natural bond angles while the ones of chitosan and glycosylated mucin (MUC) were generated using the saccharide building and sequence editor tools, respectively [34]. The individual molecules (PLGA, PEG, chitosan, and MUC) as well as the molecular complexes (PLGA-MUC and chitosan/PEG-MUC) were energy minimized and optimized using MM+ Force Field algorithm.…”

Section: In Silico Mucoadhesion Analysismentioning

confidence: 99%

How Can Biomolecules Improve Mucoadhesion of Oral Insulin? A Comprehensive Insight using Ex-Vivo, In Silico, and In Vivo Models

et al. 2020

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Currently, insulin can only be administered through the subcutaneous route. Due to the flaws associated with this route, it is of interest to orally deliver this drug. However, insulin delivered orally has several barriers to overcome as it is degraded by the stomach’s low pH, enzymatic content, and poor absorption in the gastrointestinal tract. Polymers with marine source like chitosan are commonly used in nanotechnology and drug delivery due to their biocompatibility and special features. This work focuses on the preparation and characterization of mucoadhesive insulin-loaded polymeric nanoparticles. Results showed a suitable mean size for oral administration (<600 nm by dynamic laser scattering), spherical shape, encapsulation efficiency (59.8%), and high recovery yield (80.6%). Circular dichroism spectroscopy demonstrated that protein retained its secondary structure after encapsulation. Moreover, the mucoadhesive potential of the nanoparticles was assessed in silico and the results, corroborated with ex-vivo experiments, showed that using chitosan strongly increases mucoadhesion. Besides, in vitro and in vivo safety assessment of the final formulation were performed, showing no toxicity. Lastly, the insulin-loaded nanoparticles were effective in reducing diabetic rats’ glycemia. Overall, the coating of insulin-loaded nanoparticles with chitosan represents a potentially safe and promising approach to protect insulin and enhance peroral delivery.

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“…Oral bioavailability was found to be less than 50% but the pharmacological effects of insulin have been noticed for a longer period of time with the nanoparticle as compared to parenteral insulin administration. They concluded that 10 IU/Kg of orally administered insulin given to diabetic rats which is incorporated in nanoparticles shows a maximum change in glucose level of at a time period of 5 hours [ 30 ].…”

Section: Oral Delivery Of Insulinmentioning

confidence: 99%

Emerging Trends in Noninvasive Insulin Delivery

Verma

Kumar

Malviya

et al. 2014

Journal of Pharmaceutics

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This paper deals with various aspects of oral insulin delivery system. Insulin is used for the treatment of diabetes mellitus, which is characterized by the elevated glucose level (above the normal range) in the blood stream, that is, hyperglycemia. Oral route of administration of any drug is the most convenient route. Development of oral insulin is still under research. Oral insulin will cause the avoidance of pain during the injection (in subcutaneous administration), anxiety due to needle, and infections which can be developed. Different types of enzyme inhibitors, like sodium cholate, camostat, mesilate, bacitracin, leupeptin, and so forth, have been used to prevent insulin from enzymatic degradation. Subcutaneous route has been used for administration of insulin, but pain and itching at the site of administration can occur. That is why various alternative routes of insulin administration like oral route are under investigation. In this paper authors summarized advancement in insulin delivery with their formulation aspects.

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Development and Evaluation of Insulin Incorporated Nanoparticles for Oral Administration

Cited by 32 publications

References 14 publications

Polyurethane‐incorporated chitosan/alginate core–shell nano‐particles for controlled oral insulin delivery

Polyurethane‐incorporated chitosan/alginate core–shell nano‐particles for controlled oral insulin delivery

How Can Biomolecules Improve Mucoadhesion of Oral Insulin? A Comprehensive Insight using Ex-Vivo, In Silico, and In Vivo Models

Emerging Trends in Noninvasive Insulin Delivery

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