Détermination structurale de triterpenoïdes isolés des feuilles de &lt;i&gt;Combretum glutinosium&lt;/i&gt; Perr. Ex DC (Combretaceae)

N’diaye, Daouda; Mbaye, Mbaye Diagne; Gassama, Abdoulaye; Lavaud, Catherine; Pilard, Serge

doi:10.4314/ijbcs.v11i1.39

Cited by 4 publications

(2 citation statements)

References 14 publications

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“…Les spots obtenus après élution sont observés tout d'abord à 254 nm puis à 366 nm sous lumière UV par extinction de fluorescence et ensuite révélés par pulvérisation de vanilline sulfurique suivie d'un chauffage. Le fractionnement des échantillons par chromatographie sur colonne a été réalisé sur gel de silice Chromatogel 60 SDS (granulométrie 20-45 µm) sous faible pression d'air (300 mbar) (Daouda et al, 2017).…”

Section: Méthodes Expérimentales Procédures Généralesunclassified

Identification de deux phytostérols biologiquement actifs de l’extrait cyclohexanique des feuilles de <i>Ficus sur</i> (Moraceae)

Boyode¹,

Simalou²,

Eloh³

et al. 2018

Int. J. Bio. Chem. Sci

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Tel. : (+228) 91 58 81 61 REMERCIEMENTS Nos sincères remerciements vont à l'Agence Universitaire de la Francophonie (AUF) pour le financement de ce travail. RESUMEFicus sur est une plante alimentaire couramment utilisée en médecine traditionnelle au Togo. Elle a cependant fait l'objet de très peu d'études phytochimiques. Le présent travail a pour but de valoriser cette espèce à travers l'identification continue de ses métabolites. Ainsi, l'extraction cyclohexanique au Soxhlet a été réalisée sur les feuilles de Ficus sur. Cet extrait cyclohexanique obtenu a été ensuite fractionné puis purifié par chromatographie sur colonne de gel de silice. L'identification des composés a été réalisée par GC-MS, précédée quelquefois d'une étape de dérivatisation. Treize fractions (A1 à A13) ont été obtenues du fractionnement de cet extrait. Après deux purifications successives de la fraction A5, deux sous-fractions A5. Identification of two bioactive phytosterols of the cyclohexane extract of Ficus sur (moraceae) leaves ABSTRACTFicus sur is a food plant commonly used in traditional medicine in Togo. However, few phytochemical studies of this species are available. This work aims at contributing to the enhancement of this approach through an identification of its metabolites. The leaves of Ficus sur were then extracted with cyclohexane using soxhlet. The obtained cyclohexane extract was fractionated and purified by chromatography on silica gel column. Identification of the compounds was carried out by GC-MS, sometimes preceded by a derivatization step. Thirteen fractions (A1 to A13) were obtained from the fractionation of this extract. After two successive purifications of the fraction A5, two subfractions A5.1 and A5.2 were obtained. The mass spectrum of a compound of A2 has a molecular peak at m/z 426 and peaks [M-69]

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Section: Méthodes Expérimentales Procédures Généralesunclassified

Identification de deux phytostérols biologiquement actifs de l’extrait cyclohexanique des feuilles de <i>Ficus sur</i> (Moraceae)

Boyode¹,

Simalou²,

Eloh³

et al. 2018

Int. J. Bio. Chem. Sci

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“…The traditional uses of this plant have led to numerous pharmacological studies, including antibacterial, antifungal, anthelmintic, antimalarial and antidrepanosite properties (Baba-Moussa et al, 1999;Ouattara et al, 2006;Usman et al, 2017;Sall et al, 2017;Alowanou et al, 2019). Previous phytochemical studies of the genus Combretum led to the isolation of tannins, flavonoids, triterpenoids and steroids (Jossang et al, 1994;Dawe et al, 2013;Roy et al, 2014, Amako et al, 2016Sene et al, 2018;N'Diaye et al, 2017;Balde et al, 2019). In the search for a new active ingredient effective against increasing biological resistance to synthetic anthelmintics, the study reported here was undertaken on the leaves of C. glutinosum, which were obtained from plants in Bé nin.…”

Section: Introductionmentioning

confidence: 99%

Anthelmintic flavonoids and other compounds from Combretum glutinosum Perr. ex DC (Combretaceae) leaves

et al. 2021

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A chemical study of the hydro-ethanol extract of the leaves of Combretum glutinosum resulted in the isolation of nine compounds, including 5-demethylsinensetin (1), umuhengerin (2), (20S,24R)-ocotillone (3), lupeol (4), β-sitosterol (5), oleanolic acid (6), betulinic acid (7), corymbosin (8) and β-sitosterol glucoside (9). Four compounds have been isolated for the first time from the genus Combretum [viz. (1), (2), (3) and (8)]. The crystal structures of flavonoid (2), C20H20O8, Z′ = 2, and triterpene (3), C30H50O3, Z′ = 1, have been determined for the first time; the latter confirmed the absolute configuration of native (20S,24R)-ocotillone previously derived from the crystal structures of related derivatives. The molecules of (3) are linked into supramolecular chains by intermolecular O—H...O hydrogen bonds. The crude extracts obtained by aqueous decoction and hydro-ethanolic maceration, as well as the nine isolated compounds, were tested for their anthelmintic activity on the larvae and adult worms of Haemonchus contortus, a hematophage that causes parasitic disorders in small ruminants. The evaluated anthelmintic activity showed that the extracts at different doses, as well as all the compounds tested at 150 µg ml−1, inhibited the migration of the larvae and the motility of the adult worms of the parasite compared with the phosphate buffer solution negative reference control. The best activity was obtained with flavonoids (1), (2) and (8) on both stages of the parasite. The flavones that showed good activity can be used for the further development of other derivatives, which could increase the anthelmintic efficacy.

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Antimicrobial investigation of ethnobotanically selected guinean plant species

Baldé

Tuenter

Traoré

et al. 2020

Journal of Ethnopharmacology

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Détermination structurale de triterpenoïdes isolés des feuilles de <i>Combretum glutinosium</i> Perr. Ex DC (Combretaceae)

Cited by 4 publications

References 14 publications

Identification de deux phytostérols biologiquement actifs de l’extrait cyclohexanique des feuilles de <i>Ficus sur</i> (Moraceae)

Identification de deux phytostérols biologiquement actifs de l’extrait cyclohexanique des feuilles de <i>Ficus sur</i> (Moraceae)

Anthelmintic flavonoids and other compounds from Combretum glutinosum Perr. ex DC (Combretaceae) leaves

Antimicrobial investigation of ethnobotanically selected guinean plant species

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