Determination of valganciclovir and ganciclovir in human plasma by liquid chromatography tandem mass spectrometric detection

Therapeutic drug monitoring and LC–MS/MS

“…It is therefore advantageous to be able to quantify both forms of the drug simultaneously, and LC-MS/MS assays have been developed for both groups of drugs to facilitate this. Protein precipitation is the sample preparation method of choice for all these drugs [45][46][47][48][49], although Singh et al used SPE with MCX cartridges to clean up plasma samples prior to quantification of ganciclovir and valganciclovir [50]. Ganciclovir and valganciclovir can be separated using a C18 [50] or a silica column [49], but ribavirin and viramidine are very polar molecules, and a column such as hypercarb [45], or ODS-BP [47] may be useful to increase retention times and facilitate chromatographic separation from isobaric interferents such as uridine and cytosine [45] (Table 2).…”

“…40,000 g/L (G) 10,000 g/L (V) [49] Ganciclovir and valganciclovir 200 L Acyclovir andvalaciclovir [50] inhibitor with a CI but the reason for measuring all of the drugs simultaneously is to make sample handling easier. Some methods perform direct injection of the supernatant from the protein crash [57,61,62], whilst others use on line SPE to further purify the sample [63][64][65].…”

evelopment and Validation of Few UV Spectrophotometric Methods for the determination of Valganciclovir in Bulk and Pharmaceutical Dosage Form

“…In cytomegalovirus infected cells, ganciclovir is initially phosphorylated to the monophosphate form by viral protein kinase and then it is further phosphorylated via cellular kinases to produce the triphosphate form. 1 Very few methods are reported in the literature including liquid chromato graphic methods, [2][3][4] spectrophotometric techniques 5 and LC/ MS/MS [6][7][8] methods for the determination of Valganciclovir in tablet dosage forms As there was no work in the literature about the UV spectrophotometric method for the analysis of VGC using method a, b, c and d by zero order and first order derivative. Therefore, the objective of present study was to develop and validate a simple, precise, accurate and economical UV spectrophotometric method for estimation of VGC.…”

Stability Indicating Liquid Chromatographic Method for the Quantitative Determination of Valganciclovir in Pharmaceutical Dosage Forms

“…This triphosphate form is slowly. Very few methods are reported in the literature including liquid chromatographic methods [2][3][4] , spectrophotometric techniques 5 and LC/MS/MS [6][7][8] methods for the determination of Valganciclovir in tablet dosage forms and in biological fluids. Suresh Kumar et al, have studied the Impurity profile and related substances 9 of Valganciclovir and Stefanidis et al, studied the reactivity 10 of Valganciclovir in aqueous solution.…”