Stability Indicating Liquid Chromatographic Method for the Quantitative Determination of Valganciclovir in Pharmaceutical Dosage Forms

Annapurna, Mukthinuthalapati Mathrusri; Tulasi, K Lakshmi Sai; Sirichandra, M

doi:10.22270/jddt.v3i3.498

Cited by 7 publications

(4 citation statements)

References 6 publications

(5 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…After then, it was converted to its triphosphate form by cellular kinases. 2 According to a review of the valganciclovir literature, UV methods, [1][2][3] LC-MS methods [4][5][6][7][8] and RP-HPLC methods [9][10][11][12][13] were published up to this point. The study's major objective was to create an easy, precise RP-HPLC method for the measurement of valganciclovir in both pure and tablet dose forms.…”

Section: Introductionmentioning

confidence: 99%

Analytical Method Development and Validation for Determination of Valganciclovir by Using RP-HPLC

Abhigna,

Sundararajan

2023

Int J. Pharm. Investigation

View full text Add to dashboard Cite

Objectives: A novel, precise, and accurate RP-HPLC method was developed and validated for the determination of valganciclovir in pure and medicinal dose form. Cytomegalovirus infections were treated with the antiviral medication valganciclovir. Ganciclovir was first phosphorylated by viral protein kinase into the monophosphate form in cytomegalovirus-infected cells. Afterwards, it was converted to its triphosphate form by cellular kinases. Materials and Methods: The analyte separation was obtained by Shimadzu C 18 column. Mobile phase was in the ratio (20:80v/v) of acetonitrile and 0.05% of orthophosphoric acid. Flow rate of 0.6mL/min was used. The wavelength of the drug was at 254nm with a retention time of 3. 761 min. Results: The regression equation of valganciclovir was found to be y=56286x+50633, with a R 2 value of 0. 9993. It was reported that the precision percent RSD was under 2%. The rate of valganciclovir recovery was 99.43%. Valganciclovir LOD and LOQ were determined to be 0.1 µg/mL and 0.3µg/mL, respectively. Conclusion:The proposed method was shown to be exact, accurate, and perfect for usage in QC labs for quantitative analysis of pharmaceutical dosage forms, both single and combined.

show abstract

Section: Introductionmentioning

confidence: 99%

Analytical Method Development and Validation for Determination of Valganciclovir by Using RP-HPLC

Abhigna,

Sundararajan

2023

Int J. Pharm. Investigation

View full text Add to dashboard Cite

show abstract

“…[3][4][5][6] . Literature Survey revealed that the drug has been estimated by UV-Spectrophotomtric [11][12][13] , RP-HPLC [14][15][16][17] , HPTLC [18] and Liquid chromatographic method [19][20][21] has been reported so far. The present study describes a simple, precise and accurate analytical method for the estimation of Valganciclovir hydrochloride in bulk and pharmaceutical dosage forms.…”

Section: Introductionmentioning

confidence: 99%

Development and validation of valganciclovir hydrochloride in bulk and pharmaceutical dosage form by HPTLC method

Karthik

C²,

H.G³

2020

IJAC

View full text Add to dashboard Cite

A simple, rapid and accurate High performance thin layer chromatography is described for the Development and validation of HPTLC method for Valganciclovir Hydrochloride in bulk and Pharmaceutical dosage form. The separation is carried out on Merck TLC aluminum sheets of silica gel 60 F254 using Chloroform: Methanol: Ammonia (6.5:3.4:0.1v/v) mobile phase. Quantification was done by Densitometric scanning at 254nm. The linearity was found to be the range of 100-500ng/spot for Valganciclovir hydrochloride with the correlation coefficient of 0.9993. The regression equation was found to be Y=10.168x-94.8. The Rf value of Valganciclovir hydrochloride was found to be 0.74. The LOD and LOQ were found to 9.19 and 27.87 respectively. Average recovery was found to be 99.66% which show that the method was free from interference from excipients present in the formulation. Simultaneously the Percentage relative standard deviation was well within the range of 2%. The above method was validated according to the ICH guidelines. The established method enabled accurate, precise and applied to the analysis of Valganciclovir hydrochloride in bulk and Pharmaceutical dosage form.

show abstract

“…In cytomegalovirus infected cells, ganciclovir is initially phosphorylated to the monophosphate form by viral protein kinase and then it is further phosphorylated via cellular kinases to produce the triphosphate form. 1 Very few methods are reported in the literature including liquid chromato graphic methods, [2][3][4] spectrophotometric techniques 5 and LC/ MS/MS [6][7][8] methods for the determination of Valganciclovir in tablet dosage forms As there was no work in the literature about the UV spectrophotometric method for the analysis of VGC using method a, b, c and d by zero order and first order derivative. Therefore, the objective of present study was to develop and validate a simple, precise, accurate and economical UV spectrophotometric method for estimation of VGC.…”

Section: Introductionmentioning

confidence: 99%

evelopment and Validation of Few UV Spectrophotometric Methods for the determination of Valganciclovir in Bulk and Pharmaceutical Dosage Form

Mondal¹,

Reddy²,

Mondal³

et al. 2018

PHM

View full text Add to dashboard Cite

Background: The present research paper described about the optimization of various zero order and first order UV spectrophotometric method using different buffers. Validation study was performed to develop a simple, sensitive, rapid, accurate and economical Ultra Violet spectrophotometric method for the estimation of Valganciclovir. Methods: UV 1800 double beam UV Visible Spectrophotometer with a pair of 10mm path length matched quartz cells were used for the study. Method A (Water), Method B (phosphate buffer pH2), Method C (Phosphate buffer pH4) and Method D (phosphate buffer pH5) were developed for estimation of Valganciclovir by zero-order and first-order derivative. Results: Linearity was carried out in the concentration range of 5-60 µg/ml and the correlation coefficient were found to be 0.999. The percentage recoveries were found to be 98-102%. The relative standard deviation was found to be <2%. The LOD and LOQ were found to be 0.3241 µg/ml and 0.8227 µg/ml respectively. Conclusion: Hence, the methods were validated according to ICH guidelines and can be adopted for the routine analysis of Valganciclovir in pure and tablet dosage form.

show abstract

Stability Indicating Liquid Chromatographic Method for the Quantitative Determination of Valganciclovir in Pharmaceutical Dosage Forms

Cited by 7 publications

References 6 publications

Analytical Method Development and Validation for Determination of Valganciclovir by Using RP-HPLC

Analytical Method Development and Validation for Determination of Valganciclovir by Using RP-HPLC

Development and validation of valganciclovir hydrochloride in bulk and pharmaceutical dosage form by HPTLC method

evelopment and Validation of Few UV Spectrophotometric Methods for the determination of Valganciclovir in Bulk and Pharmaceutical Dosage Form

Contact Info

Product

Resources

About