Determination of total mercury in biological samples using flow injection CVAAS following tissue solubilization in formic acid

Kan, Masahiko; Willie, Scott; Scriver, Christine; Sturgeon, Ralph E.

doi:10.1016/j.talanta.2005.07.027

Cited by 34 publications

(20 citation statements)

References 12 publications

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“…Also the use of formic acid in similar applications has provided good results. 17,29 As shown in this work (Table 1), similar results in the medium of TMAH were obtained by analysis of processed meats using acid digestion and formic acid solubilization. The concentrations obtained using the three different methods of sample preparation are in agreement for all the studied analytes, the values of the concentrations obtained with the respective methods did not vary more than 5%.…”

Section: Comparison Of Sample Preparation Proceduressupporting

confidence: 86%

Determination of Ca, Cu, Fe and Mg in fresh and processed meat treated with tetramethylammonium hydroxide by atomic absorption spectrometry

Nunes¹,

Acunha²,

Oreste³

et al. 2011

J. Braz. Chem. Soc.

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Um método simples para o tratamento de amostras de carne fresca e processada com hidróxido de tetrametilamônio (TMAH) é proposto para a determinação de Ca, Fe e Mg por espectrometria de absorção atômica com chama (FAAS) e Cu por espectrometria de absorção atômica com forno de grafite (GFAAS). A exatidão foi avaliada por comparação dos resultados usando outros dois procedimentos de preparo de amostra e por análise de materiais de referência certificados. Não houve diferença significativa entre os resultados obtidos, em um nível de confiança de 95%. Os limites de detecção para Ca, Cu, Fe e Mg foram 45,0, 0,2, 16,0 e 0,3 µg g -1 , respectivamente. A espectrometria de absorção atômica de alta resolução com fonte contínua em forno de grafite (HR-CS GFAAS) foi empregada para avaliar as interferências espectrais na determinação de Cu. Entretanto, nenhuma interferência foi encontrada. O método proposto é simples, rápido e confiável para análise de produtos cárneos e não requer o uso de equipamentos especiais nem de ácidos fortes no preparo das amostras.A simple method for treating fresh and processed meat with tetramethylammonium hydroxide (TMAH) is proposed for the determination of Ca, Fe and Mg by flame atomic absorption spectrometry (FAAS) and Cu by graphite furnace atomic absorption spectrometry (GFAAS). The accuracy was evaluated by comparison of the results by using two other sample preparation procedures and by the analysis of certified reference materials. No significant differences between the results were found at the 95% confidence level. Limits of detection for Ca, Cu, Fe and Mg were 45.0, 0.2, 16.0 and 0.3 µg g -1 , respectively. High-resolution continuum source graphite furnace atomic absorption spectrometry (HR-CS GFAAS) was employed to evaluate the spectral interference in the determination of Cu. However, no interference was found. The proposed method is simple, fast and reliable for meat products analysis and does not require especial equipment, neither strong acid for sample preparation.

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Section: Comparison Of Sample Preparation Proceduressupporting

confidence: 86%

Determination of Ca, Cu, Fe and Mg in fresh and processed meat treated with tetramethylammonium hydroxide by atomic absorption spectrometry

Nunes¹,

Acunha²,

Oreste³

et al. 2011

J. Braz. Chem. Soc.

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“…The determination of Hg and MeHg in seafood samples has been done using many tecniques [3][4][5][6] and the quality of the results can be guarantee by the use of certified reference materials (CRMs).…”

Section: Introductionmentioning

confidence: 99%

Estimation of uncertainty to the homogeneity study in the certification of a candidate certified reference material in fish matrix

Ulrich

Sarkis

2013

16th International Congress of Metrology

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Abstract.A homogeneity study in the preparation of a fish candidate certified reference material is reported. In the present study, homogeneity was examined by flow injection analysis cold vapour atomic absorption spectrometry technique (FIA-CV-AAS). Total mercury and methylmercury were studied in this study. Univariate analysis of variance (ANOVA) was performed for all analytes. The results were evaluated using an F-test, which demonstrated no significant difference for the between-bottle results. It is indicative that this material is homogeneous. The results of the between-bottle homogeneity study provide for the evaluation of one of the uncertainty components in the certification process.

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“…In addition, it can be used in closed flasks and in most cases without the need of external energy for solubilization of the samples, while the assistance of ultrasound can be used to reduce acid consumption and sample preparation time. [25][26][27][28][29] Considering the importance of certain analytical data to both consumers and health professionals, and also the recent legislations for Na concentration in foods, this work focus on the sample preparation of meat based products in order to simplify the procedures available for the determination of Na and K by atomic spectrometric techniques. For this purpose, two methods were evaluated to prepare a slurry sample: (i) using the alkaline solubilization with TMAH and (ii) using acidic solubilization with formic acid in order to determine the better alternative method in relation to the conventional procedures of mineralization.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of sample preparation methods based on alkaline and acid solubilization for the determination of Na and K in meat samples by atomic spectrometric techniques

Silva¹,

Nunes²,

Oreste³

et al. 2012

J. Braz. Chem. Soc.

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Foram avaliados dois métodos diferentes de preparo de amostras de carnes baseados na solubilização em meios alcalino ou ácido. O objetivo deste estudo foi determinar os teores de Na e K após solubilização com hidróxido de tetrametilamônio (TMAH) à temperatura ambiente, e com ácido fórmico e aquecimento em bloco de digestão a 50 ºC por cerca de 2 h. A solubilização alcalina se apresentou como uma metodologia simples e robusta e a melhor alternativa frente aos procedimentos de mineralização convencional, permitindo a solubilização completa das amostras usando pequenas quantidades de TMAH. O método foi validado empregando-se materiais de referência certificada assim como pela comparação com método de digestão convencional com ácido nítrico. Os limites de detecção obtidos foram de 0,8 e 2,0 mg g -1 para Na e K, respectivamente, e se mostraram adequados para o objetivo das análises.Two different sample preparation methods were evaluated for meat samples employing the solubilization in alkaline or acidic media. The aim of this study was to determine Na and K levels after sample solubilization with tetramethylammonium hydroxide (TMAH) at room temperature, and with formic acid and heating in a digester block at 50 ºC for ca. 2 h. The alkaline solubilization showed to be a simple and robust method and the better alternative in relation to the mineralization conventional procedures, allowing the complete solubilization of the samples even when small quantities of TMAH were used. The method was validated by using certified reference material as well as by comparing with samples digested with nitric acid. The limits of detection obtained were 0.8 and 2.0 μg g -1 for Na and K, respectively, and showed to be adequate for the analysis purposes.

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Determination of total mercury in biological samples using flow injection CVAAS following tissue solubilization in formic acid

Cited by 34 publications

References 12 publications

Determination of Ca, Cu, Fe and Mg in fresh and processed meat treated with tetramethylammonium hydroxide by atomic absorption spectrometry

Determination of Ca, Cu, Fe and Mg in fresh and processed meat treated with tetramethylammonium hydroxide by atomic absorption spectrometry

Estimation of uncertainty to the homogeneity study in the certification of a candidate certified reference material in fish matrix

Evaluation of sample preparation methods based on alkaline and acid solubilization for the determination of Na and K in meat samples by atomic spectrometric techniques

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