Determination of the Insecticide Imidacloprid in Potato (Solanum tuberosum L.) and Onion (Allium cepa) by High-Performance Liquid Chromatography with Diode-Array Detection

Abstract

A high-performance liquid chromatography (HPLC) method has been proposed for the determination of imidacloprid residues in fortified potato ( Solanum tuberosum L.) and onion ( Allium cepa ) and in treated potato and onion samples. Sample preparation consisted of dichloromethane extraction of imidacloprid from the plant material, followed by purification of the obtained onion extract on an LC-Florisil disposable cartridge. The obtained potato extracts were further analyzed without purification. The HPLC-diode-array-detection (DAD) method has been developed on reversed phase for separation of imidacloprid with isocratic elution with a mixture of 0.01 M phosphate buffer (pH 3.15) and acetonitrile (75 : 25, v/v). Spectral data obtained with DAD allow the identification of imidacloprid residue. Detector response showed a linear dynamic range from 0.0125 to 0.500 µ g/mL. Imidacloprid was recovered from fortified samples in a range of 94-99%. The within-day RSD of repeatability of the retention time of imidacloprid standard solution was less than 0.1% and of the obtained peak area less than 5%. For potato and onion samples, the limits of detection were 0.0075 and 0.0060 mg/kg and the limits of determination were 0.015 and 0.012 mg/kg, respectively.

Determination of selected neonicotinoid insecticides by liquid chromatography with thermal lens spectrometric detection

“…A mixture of water and acetonitrile is commonly used for the HPLC separation of different neonicotinoids on a reverse phase (Mandić et al 2005;Rancan et al 2006). In the procedure of mobile phase optimization it was found that the increase of acetonitrile content, e.g.…”

“…However, because of the thermolability and low volatility of neonicotinoids, the GC/MS procedures involve complex sample preparation. Hence, liquid chromatographic methods such as HPLC/DAD (Obana et al 2002;Mandić et al 2005), HPLC/MS (Obana et al 2003;Fidente et al 2005;Seccia et al 2005), and HPLC/post-column photoactivation/electrochemical detection (Rancan et al 2006) appeared to be advantageous for the determination of neonicotinoids in various matrices.…”

Dissipation rate of acetamiprid in sweet cherries

“…However, GC determination can produce overestimated values of highly polar compounds such as acetamiprid because of the matrix-induced enhancement effect (Sasaki et al, 1998). For determination of other neonicotionoids, such as imidacloprid, the highperformance liquid chromatography is already commonly used (Mandić et al, 2005). In this study, acetamiprid was determined by HPLC.…”

Application of liquid chromatography with diode-array detector for determination of acetamiprid and 6-chloronicotinic acid residues in sweet cherry samples

“…Ferrer, I., Thurman, E.M. and Fernandez-Alba, A.R. : Quantitation and accurate mass analysis of pesticides in vegetables by LC/TOF-MS. Analytical Chemistry, 77: 2818-2825, 2005 Analysis of nicotinoid insecticides residues in honey by solid matrix partition clean-up and liquid chromatography-electrospray mass spectrometry. Journal of Chromatography A, 1094: 175-178, 2005.…”

“…For this reason, the concentration of neonicotinoid residues, including their metabolites, in agricultural products should be monitored. This requires appropriate methods of extraction and determination, such as the already existing methods for imidacloprid in potato and onion (Mandić et al, 2002(Mandić et al, , 2005Lazić et al, 2002Lazić et al, , 2003Lazić et al, , 2004, or thiamethoxam in pepper (Lazić et al, 2002a).…”