Dissipation rate of acetamiprid in sweet cherries

Abstract

SUMMARYDegradation of acetamiprid in sweet cherry samples was evaluated at several intervals from the product application until the end of the pre-harvest interval. An orchard of sweet cherries located at Stepanovićevo village near Novi Sad was used in this study. Acetamiprid was applied according to the manufacturer's recommendation for protecting sweet cherries from their most important pests. Sweet cherry fruit samples were collected at eight intervals: immediately after acetamiprid application and 2, 4, 6, 8, 10, 12 and 14 days after application. The extraction of acetamiprid from sweet cherry samples was performed using a QuEChERS-based method. Determination was carried out using an HPLC-UV diode array detection system (Agilent 1100, United States) with an Agilent Zorbax Eclipse C18 column (50 mm × 4.6 mm internal diameter, 1.8 µm particle size). The method was subjected to a thorough validation procedure. The recovery data were obtained by spiking blank sweet cherry samples at three concentration levels (0.1-0.3 mg/ kg), yielding 85.4% average recovery. Precision values expressed as relative standard deviation (RSD) were below 1.61% for the intraday precision. Acetamiprid showed linear calibrations from 0.05 to 2.5 µg/ml with correlation coefficient (R 2 ) of 0.995%. The limit of detection and limit of quantification were found to be 5 µg/kg and 14 µg/kg, respectively. The validated method was applied in the analysis of acetamiprid in sweet cherry samples. During the study period, the concentration of acetamiprid decreased from 0.529 mg/kg to 0.111 mg/kg. The content of acetamiprid in sweet cherry samples at the end of the pre-harvest interval was below the maximum permissible level specified by the Serbian and EU MRLs.

Validation of Individual-Based Markov-Like Stochastic Process Model of Insect Behavior and a “Virtual Farm” Concept for Enhancement of Site-Specific IPM

“…The insects present in or entering pesticide application zone were deemed exposed to additional mortality risks. The date and area of each application were taken into account, along with the estimated temporal profiles of its residues (Lazić et al, 2014 ) translated into mortality rates of adult flies, and whenever relevant, immature stages developing in the fruit. In such the areas, mortality risk due to the local natural enemies was temporarily reduced to reflect patterns of the pesticide-imposed transient suppression.…”

Evaluation of Dissipation Behavior, Residues, and Dietary Risk Assessment of Fludioxonil in Cherry via QuEChERS Using HPLC-MS/MS Technique

“…Similar methods were also widely used in the detection of other pesticide residues in cherries. A previous study found that the QuEChERS method coupled with LC/MS/MS can effectively detected the residues of acetamprid in cherries, and the average recovery was from 80.12 to 98.04% [43]. A combination of solid phase microextraction (SPME) and LC/MS was used to determine the residues of five fungicides (trichlorfon, fluchondrion, o-phenol, pretilachlor, and toluene) in cherries.…”

Revised paper: Neonicotinoids as a potential threat to honey: Development of liquid chromatography methods for their determination

“…Having that in mind, determination of neonicotinoids in honey has become necessary and requires sensitive, selective and efficient analytical methods. The residue of neonicotinoids in honey samples can be measured by liquid chromatography (LC) coupled to different detectors such as diode array detector (DAD) (Campillo et al, 2013;Jovanov et al, 2015) or mass spectrometer (MS or MS/MS) (Fidente et al, 2005;Jovanov et al, 2013;Jovanov et al, 2014;Lazić et al, 2014). The MS/MS detector assures high sensitivity and selectivity compared to DAD, but it is an expensive and high maintenance instrument.…”