Determination of tetracycline residues in chicken meat by liquid chromatography-tandem mass spectrometry

Çetinkaya, Figen; Yibar, Artun; Soyutemiz, G. Ece; Okutan, Bülent; Özcan, Ayşegül; Karaca, Meryem

doi:10.1080/19393210.2012.655782

Cited by 35 publications

(21 citation statements)

References 20 publications

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“…Another study was performed in 2012 on 60 chicken meat samples, taken from supermarkets in Bursa, by analyzing tetracycline group antibiotics with liquid chromotography-double mass spectrometer and doxycycline was found in 4 and tetracycline was Emre ARSLANBAŞ found in 1 of the samples. No oxytetracycline and chlortetracycline was found in any of the samples (7). It is seen that the results of this study are similar, in terms of MRL, to the results of the studies, performed by Yıldırım et al (24) and Cetinkaya et al (7).…”

Section: Discussionsupporting

confidence: 88%

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Determination of Some Antibiotic Residues by HPLC Method in Chicken Meats Prepared for Consumption

Arslanbaş¹

2018

Erciyes Üniversitesi Veteriner Fakültesi Dergisi

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In this study, it is aimed to search residues of enrofloxacin, doxycycline and tylosin which are widely used in poultry farming in fresh and packaged chicken meat samples taken from five brands being sold nationwide, through HPLC method. Enrofloxacin, doxycycline and tylosin levels in the samples of chicken meat were evaluated according to "The Regulation on Classification of Active Pharmacologic Substances that might be Available in Food of Animal Origin in Turkish Food Codex and Maximum Residue Limits" and international limits. Maximum residue limit (MRL) of enrofloxacin, doxycycline and tylosin in muscle tissues of chickens must be 100 µg/kg according to applicable regulations and international limits. It was found in totally 300 analyzed samples (whole chicken, drumstick and breast meat) that 11 (3.6%) of them had enrofloxacin, doxycycline and tylosin levels higher than MRL (between 100-150 µg/kg). Residue levels were less than allowed limits in other samples. Six (2%) of these 11 samples had enrofloxacin, 3 (1%) had doxycycline and 2 (0.6%) had tylosin residues. Since the results of this study showed that the majority of samples were lower than allowed limits, these results were considered as positive. However, as long as medicines do continue to be used for animals' health, there will always be a risk of existence of medicine residues in food of animal origin. Many stakeholders have a role in preventing residue risk arising out of veterinary medicines and all these persons must pay necessary attention to residue purification duration, training programs and keeping regular records of animals. Tüketime Sunulan Tavuk Etlerindeki Bazı Antibiyotik Kalıntılarının HPLC Yöntemi ile BelirlenmesiÖzet: Bu çalışmada, kanatlı hayvan yetiştiriciliğinde yaygın olarak kullanılan enrofloksasin, doksisiklin ve tilosin kalıntılarının, satışa sunulan beş farklı ulusal firmaya ait paketlenmiş taze tavuk eti örneklerinde HPLC yöntemiyle araştırılması amaçlanmıştır. Tavuk eti örneklerindeki enrofloksasin, doksisiklin ve tilosin düzeyleri, "Türk Gıda Kodeksi Hayvansal Gıdalarda Bulunabilecek Farmakolojik Aktif Maddelerin Sınıflandırılması ve Maksimum Kalıntı Limitleri Yönetmeliği"ne ve uluslararası limitlere göre değerlendirilmiştir. İlgili yönetmelikte ve uluslararası limitlerde enrofloksasin, doksisiklin ve tilosin için tavuk kas dokuda maksimum kalıntı limiti (MKL) 100 µg/kg olarak verilmiştir. Analizi yapılan toplam 300 örneğin (bütün tavuk, but eti ve göğüs eti) 11 (%3.6)'inde farklı örneklerde ve düzeyde MKL üzerinde (100-150 µg/kg arasında) enrofloksasin, doksisiklin ve tilosin bulunmuştur. Diğer örneklerde ise izin verilen limitlerin altında belirlenmiştir. Antibiyotik kalıntısı tespit edilen 11 örneğin 6 (%2)'sı enrofloksasin, 3 (%1)'ü doksisiklin ve 2 (%0.6)'si tilosin olarak saptanmıştır. Çalışmadan elde edilen sonuçların, büyük oranda izin verilen limitlerin altında kalması olumlu bir sonuç olarak değerlendirilmiştir. Ancak, hayvan sağlığı için ilaç kullanımı söz konusu olduğu sürece, hayvansal gıdalarda ilaç kalıntıla...

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Section: Discussionsupporting

confidence: 88%

“…No oxytetracycline and chlortetracycline was found in any of the samples (7). It is seen that the results of this study are similar, in terms of MRL, to the results of the studies, performed by Yıldırım et al (24) and Cetinkaya et al (7).…”

Section: Discussionsupporting

confidence: 88%

Determination of Some Antibiotic Residues by HPLC Method in Chicken Meats Prepared for Consumption

Arslanbaş¹

2018

Erciyes Üniversitesi Veteriner Fakültesi Dergisi

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“…In contrast, the meat were accepted within the permissible limit Table (2). Relatively higher results were obtained by Hosnia et al (2015) who recorded that, highest levels of DOC residues in liver was 20.43 μg/kg. However, lower results were reported by Cetinkayaa et al (2012) where the levels of DOC residues (µg/kg) ranged from 19.9 to 35.6 µg/kg in chicken meat samples.…”

Section: Quantization Of the Amount Of Antimicrobial Residue By Peak mentioning

confidence: 55%

Assessment of Doxycycline and Oxytetracycline Residues in Broiler Meat

Elbayoumi¹,

Yuosief²,

Elbagory³

2018

AJVS

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Key words:Doxycycline, Oxytetracycilne, poultry meat, giblet.This study aims to detect the residues of Doxycycline, Oxytetracycline in broiler meat and giblet, using high performance liquid chromatography (HPLC), Doxycycline (DOC) residues were detected in 25%, 55 % and 70 % from the examined samples of meat, liver and kidney, respectively. Moreover, the levels of DOC residues (µg/kg) ranged from 3.4 to 15.9 µg/kg with a mean value 8.39 ± 0.14 µg/kg for meat,13.3 to 2753.5 µg/kg with a mean value 1142.85 ± 21.09 for liver and from 25.1 to 3661.6 µg/kg with a mean value 1305.59 ± 27.68 µg/kg for kidney. Oxytetracycline residues were detected in 25%, 60% and 45% of meat, liver and kidney, respectively. Moreover, the levels of oxytetracycline residues (µg/kg) ranged from 0.2 to 128.1 µg/kg with a mean value 37.52 ± 0.41 µg/kg for meat, 34.5 to 1248.0 µg/kg with a mean value 435.31 ± 12.86 µg/kg for liver and 26.8 to 412.7 µg/kg with a mean value 162.23 ± 5.35 µg/kg for kidney. Also this study revealed that frying is more efficient than boiling in decrease the residue limit of these two antibiotics.

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“…Salehzadeh et al (2006) revealed that 25 (27.77%) of 90 chicken meat samples in Iran, which were detected by HPLC contained OTC concentrations that exceeding the MRL, whereas Shahid et al (2007) found that 4 out of 7 samples of chicken meat in Pakistan also positive for OTC residue in a concentration range of 30 to 85 ppb. Differently, the research done by Cetinkaya et al (2012) in Turkey with the use of LCMSMS, did not find any residue on 60 samples analyzed.…”

Section: Otc Residue In Marketed Chicken Meat Samplesmentioning

confidence: 99%

Detection of Oxytetracycline in Broiler Chicken Meat Marketed in Several Cities in Java Island Using Enzyme-Linked Immunosorbent Assay (Elisa) Method

Widiastuti

Anastasia

2015

J. Indonesian Trop. Anim. Agric.

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ABSTRAKOksitetrasiklin (OTC) adalah salah satu jenis antibiotika golongan tetrasiklin berpektrum luas yang banyak digunakan pada industri ternak ayam. Namun penyalahgunaan dengan dosis berlebihan berpotensi terhadap terbentuknya residu pada produk ternak yang dapat membahayakan konsumen berupa reaksi alergis maupun resistensi. Penelitian ini bertujuan untuk mendeteksi residu OTC dalam daging ayam pedaging asal pasar tradisional dan swalayan di Depok-Bekasi, Bandung, Cilegon, Surakarta dan Yogyakarta secara indirect competitive enzyme-linked immunosorbent assay (icELISA). Hasil analisis pada 67 daging ayam pedaging menunjukkan hanya ada 1 (1,5%) sampel yang terdeteksi adanya residu OTC sebesar 86,1 ng/g dan masih di bawah batas maksimum residu OTC yang diijinkan (100 ng/g). Meskipun demikian, penerapan regulasi yang ketat terhadap penggunaan OTC ini di bidang peternakan ayam serta monitoring keberadaan residunya pada produk ternak sebelum dipasarkan sangat diperlukan untuk mencegah pengaruh buruk dari keberadaan residu tersebut pada produk pangan asal ternak. Kata kunci : oksitetrasiklin (OTC) residu, icELISA, daging ayam pedaging ABSTRACTOxytetracycline (OTC) is one of the tetracycline (TCs) broad-spectrum antibiotics widely used in the chicken industry. However, improper use of OTC with excessive doses potentially leads to residue formation in animal products that can be harmful to consumers in the form of allergic reaction or resistance. This study aimed to detect OTC residues in broiler chicken meat, marketed in traditional markets and supermarkets in Depok, Bekasi, Bandung, Cilegon, Surakarta and Yogyakarta using indirect competitive enzyme-linked immunosorbent assay (icELISA) method. The analyses of 67 broiler meat samples showed only 1 (1.5%) sample was positive for the OTC residue at 86.1 ng/g which meant below the maximum residue limits of permissible OTC (100 ng/g). Nevertheless, a stricter regulation for the use of OTC in the poultry industry and the monitoring of its residue in chicken products prior to marketing is still necessary to avoid the adverse effects of the residue present in animal products.

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Determination of tetracycline residues in chicken meat by liquid chromatography-tandem mass spectrometry

Cited by 35 publications

References 20 publications

Determination of Some Antibiotic Residues by HPLC Method in Chicken Meats Prepared for Consumption

Determination of Some Antibiotic Residues by HPLC Method in Chicken Meats Prepared for Consumption

Assessment of Doxycycline and Oxytetracycline Residues in Broiler Meat

Detection of Oxytetracycline in Broiler Chicken Meat Marketed in Several Cities in Java Island Using Enzyme-Linked Immunosorbent Assay (Elisa) Method

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