Determination of Some Antibiotic Residues by HPLC Method in Chicken Meats Prepared for Consumption

Arslanbaş, Emre

doi:10.32707/ercivet.477315

Cited by 5 publications

(9 citation statements)

References 11 publications

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“…Different groups of drugs have different withdrawal periods and their residual action in the animal body acts at different times (Khatun et al, 2018). The presence of residual tetracycline in poultry meat and organs higher than the maximum residue limit was reported in the literature due to improper use of antibiotics by producers which is a great concern to the consumers and harmful for public health globally (Bahmani et al, 2020;Arslanbas et al, 2018;Shahbazi et al, 2015;Ibrahim et al, 2015). However, the present study revealed that poultry meat samples are free of residual tetracyclines and safe for consumption which indicated that poultry farmers maintain the proper doses and withdrawal period of the antibiotics.…”

Section: Resultsmentioning

confidence: 99%

Investigation of tetracycline residues in poultry meat samples from Dhaka city by high-performance liquid chromatography

Parvin

Shoeb

Nahar

et al. 2022

crbb

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The objective of the study was to quantify tetracycline (TCs) i.e., oxytetracycline (OTC), tetracycline (TC) and chlortetracycline (CTC) residues in thirty poultry meat samples (n = 30) collected from the local market and super shop around the Dhaka University campus during May 2019 to January 2020. Three samples were collected from each of ten locations. All samples were extracted by Quick, Easy, Cheap, Effective, Rugged and Safe (QuEChERS) method. Samples were analyzed by reversed-phase High-Performance Liquid Chromatograph equipped with Photo Diode Array Detector (LC-PDA) and a reported method was validated with good linear correlation coefficients of standards and matrix-matched calibration curves with r2=1.00, 0.99, 0.99 and r2= 0.99, 0.99,0.99 in the linearity range of 0-10 µg/kg for OTC, TC and CTC, respectively. The limit of detection (LOD) and limit of quantification (LOQ) for OTC, TC, and CTC were 1.05, 1.17, and 1.09 μg/kg and 3.15, 3.51 and 3.27 μg/kg, respectively. Accuracy that is expressed by the recovery percentages were calculated at two different concentrations (2.5 and 5 μg/kg) were 91 and 100%, 102 and 100%, and 106 and 100% for OTC, TC, and CTC, respectively. Intra-day (n=3) and inter-day (n=9, 3 days) precision data were under 10% for all sample matrixes. Standard deviations were calculated ±0.06, ±0.11 and ±0.03 and precision (expressed by RSD%) were found 5.57, 9.14 and 2.35%, respectively for OTC, TC and CTC. The HPLC-PDA method is affordable for screening of large number meat samples for residual antibiotics in biological matrices by any laboratories. The method is also cheaper in comparison with LC-MSMS. Analysis of real 30 poultry meat samples showed that the tetracyclines residues were below the quantification limit in all samples.

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Section: Resultsmentioning

confidence: 99%

Investigation of tetracycline residues in poultry meat samples from Dhaka city by high-performance liquid chromatography

Parvin

Shoeb

Nahar

et al. 2022

crbb

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“…It was then diluted with 500 μL of extract with 2 mL of ultrapure water type I (final concentration of methanol is 14% v / v ). Finally, it was then filtered by passing it through a 0.45 μm membrane filter [ 38 ].…”

Section: Methodsmentioning

confidence: 99%

Development of IgY-Based Indirect Competitive ELISA for the Detection of Fluoroquinolone Residues in Chicken and Pork Samples

Yadoung

Ishimatsu

et al. 2022

Antibiotics

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Fluoroquinolones (FQs) are among the antibiotics whose widespread use in farm-raised animals results in potentially harmful residues in the end products. Additionally, most Thai farmers use antibiotics. Amoxicillin and enrofloxacin were commonly used by pig farms, and hens were given enrofloxacin to prevent immunization side effects. Moreover, antibiotic overuse has harmed food safety in the long term, and the use of low-dose antibiotics causes bacterial resistance. Herein, an indirect competitive enzyme-linked immunosorbent assay (icELISA) was used to make a fast, easy, sensitive, and cost-effective method for monitoring FQs residues. After immunizing hens with mixed multi-hapten ciprofloxacin-bovine serum albumin (CPFX-BSA) with norfloxacin-bovine serum albumin (NFX-BSA), the IgY antibody purified from egg yolk was used for the detection of FQs residues in chicken and pork samples. The efficiency of the IgY antibody showed excellent sensitivity, with 50% inhibitory concentration (IC50) of enrofloxacin at 0.05 µg/mL, far below the MRLs defined by the EU for muscle samples, which was not to exceed 100 µg/kg. The recovery range for chicken muscle samples spiked with ENFX concentrations of 1.00–0.01 µg/mL was 86.65–112.71%, similar to pork samples, which were 84.24–117.22.2%. This method has a lot of potential for analyzing fluoroquinolones in complex samples quickly, easily, and at a low cost on-site. The IgY-based ic ELISA was developed to detect ciprofloxacin (CPFX), norfloxacin (NFX), and enrofloxacin (ENFX) residues; it confirms that IgY could be a promising choice for the detection of antibiotic residues in food samples.

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“…For multi‐class analysis, ACN has been used to extract some antibiotics from different meat tissues (Saito‐Shida, Sakai, Nemoto, & Akiyama, 2017; Tajabadi, Ghambarian, Yamini, & Yazdanfar, 2016) sometimes combined with water (Casey, Andersen, Williams, Nickel, & Ayres, 2020; Lehotay & Lightfield, 2018; Moretti et al., 2016; Song et al., 2017; Yin et al., 2016; Y. Zhang et al., 2018), EDTA (Dasenaki, Michali, & Thomaidis, 2016; Moretti et al., 2016; Yin et al., 2016; Zhao, Lucas, Long, Richter, & Stevens, 2018; Zheng et al., 2019), aqueous ammonium solutions (Dasenaki et al., 2016; Kang et al., 2017), TCA (Dasenaki et al., 2016; Rizzetti, de Souza, Prestes, Adaime, & Zanella, 2017), formic acid (He, Song, Zhou, & Zhao, 2017; Pugajeva, Ikkere, Judjallo, & Bartkevics, 2019; L. Zhao et al., 2018), dimethyl sulfoxide (Zhao et al., 2018), acetic acid (M. Zhang et al., 2018), or ethyl acetate (Yoshikawa et al., 2017). In addition, other solvents such as MeOH with formic acid (Danezis, Anagnostopoulos, Liapis, & Koupparis, 2016) or EDTA (Arslanbaş, 2018), PBS (Oyedeji, Msagati, Williams, & Benson, 2020) with TCA (Sarker et al., 2018), or McIlvaine buffer (Zhang et al., 2016) have been used. For enrichment and purification steps, the use of traditional SPE (Dasenaki et al., 2016; J.…”

Section: Analytical Methodologies For the Analysis Of The Principal A...mentioning

confidence: 99%

“…For multi‐residue determination, LC coupled to MS is the most used technique (Casey et al., 2020; Q. Chen et al., 2019; Danezis et al., 2016; Dasenaki et al., 2016; Desmarchelier et al., 2018; He et al., 2017; Jammoul & Darra, 2019; Kang et al., 2017; Lehotay & Lightfield, 2018; Moretti et al., 2016; Pugajeva et al., 2019; Qin et al., 2017; Rizzeti et al., 2017; Saito‐Shida et al., 2017; Yin et al., 2016; Yoshikawa et al., 2017; Zhang et al., 2016; M. Zhang et al., 2018; Y. Zhang et al., 2018; L. Zhao et al., 2018; Zheng et al., 2019) although LC‐UV (Arslanbaş, 2018; Oyedeji et al., 2020; Song et al., 2017; Tajabadi et al., 2016; G. N. Wang et al., 2017), TLC (Sarker et al., 2018), or commercial biochips (Gaudin, Hedou, Soumet, & Verdon, 2016) have been also employed. A summary of the most common studies is included in Table 8.…”

Section: Analytical Methodologies For the Analysis Of The Principal A...mentioning

confidence: 99%

“…Zhao et al, 2018), dimethyl sulfoxide (Zhao et al, 2018), acetic acid (M. , or ethyl acetate (Yoshikawa et al, 2017). In addition, other solvents such as MeOH with formic acid (Danezis, Anagnostopoulos, Liapis, & Koupparis, 2016) or EDTA (Arslanbaş, 2018), PBS (Oyedeji, Msagati, Williams, & Benson, 2020) with TCA (Sarker et al, 2018), or McIlvaine buffer (Zhang et al, 2016) have been used. For enrichment and purification steps, the use of traditional SPE (Dasenaki et al, 2016;Oyedeji et al, 2020;Pugajeva et al, 2019;Saito-Shida et al, 2017;Yoshikawa et al, 2017;Zhang et al, 2016;Zhao et al, 2018;Zheng et al, 2019), DSPE (Casey et al, 2020;Kang et al, 2017), MSPD , MISPE (Song et al, 2017), QuEChERS (Chen, Pan, Huang, Han, & Zhou, 2019;Desmarchelier et al, 2018;Jammoul & Darra, 2019;Qin et al, 2017), and hollow fiber-based liquid phase microextraction (Tajabadi et al, 2016) have been described.…”

Section: Multi-residue Analysismentioning

confidence: 99%

See 1 more Smart Citation

Determination of antibiotics in meat samples using analytical methodologies: A review

Moga

Vergara-Barberán

Lerma‐García

et al. 2021

Comp Rev Food Sci Food Safe

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Antibiotics are widely used to prevent or treat some diseases in human and veterinary medicine and also as animal growth promoters. The presence of these compounds in foods derived from food‐producing animals can be a risk for human health. Consequently, regulatory agencies have set maximum residue limits for antibiotics in food samples. Therefore, the development of novel methodologies for its determination in food samples is required. Specifically, the analysis and quantification of these substances in meat tissues is a challenge for the analytical chemistry research community. This is due to the complexity of the matrix and the low detection limits required by the regulatory agencies. In this sense, a comprehensive review on the development of new sample preparation treatments involving extraction, cleanup, and enrichment steps of antibiotics in meat samples in combination with sensitive and sophisticated determination techniques that have been carry out in the last years is necessary. Therefore, the aim of this work is to summarize the published methodologies for the determination of antibiotics from 2016 until the beginning of the second semester of 2020. The first part of this review includes an introduction about antibiotic families, followed by sample preparation and determination techniques applied to the different families. Finally, a detailed discussion of the current trends and the future possible perspectives in this field are also included.

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Determination of Some Antibiotic Residues by HPLC Method in Chicken Meats Prepared for Consumption

Cited by 5 publications

References 11 publications

Investigation of tetracycline residues in poultry meat samples from Dhaka city by high-performance liquid chromatography

Investigation of tetracycline residues in poultry meat samples from Dhaka city by high-performance liquid chromatography

Development of IgY-Based Indirect Competitive ELISA for the Detection of Fluoroquinolone Residues in Chicken and Pork Samples

Determination of antibiotics in meat samples using analytical methodologies: A review

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