ABSTRAKOksitetrasiklin (OTC) adalah salah satu jenis antibiotika golongan tetrasiklin berpektrum luas yang banyak digunakan pada industri ternak ayam. Namun penyalahgunaan dengan dosis berlebihan berpotensi terhadap terbentuknya residu pada produk ternak yang dapat membahayakan konsumen berupa reaksi alergis maupun resistensi. Penelitian ini bertujuan untuk mendeteksi residu OTC dalam daging ayam pedaging asal pasar tradisional dan swalayan di Depok-Bekasi, Bandung, Cilegon, Surakarta dan Yogyakarta secara indirect competitive enzyme-linked immunosorbent assay (icELISA). Hasil analisis pada 67 daging ayam pedaging menunjukkan hanya ada 1 (1,5%) sampel yang terdeteksi adanya residu OTC sebesar 86,1 ng/g dan masih di bawah batas maksimum residu OTC yang diijinkan (100 ng/g). Meskipun demikian, penerapan regulasi yang ketat terhadap penggunaan OTC ini di bidang peternakan ayam serta monitoring keberadaan residunya pada produk ternak sebelum dipasarkan sangat diperlukan untuk mencegah pengaruh buruk dari keberadaan residu tersebut pada produk pangan asal ternak. Kata kunci : oksitetrasiklin (OTC) residu, icELISA, daging ayam pedaging ABSTRACTOxytetracycline (OTC) is one of the tetracycline (TCs) broad-spectrum antibiotics widely used in the chicken industry. However, improper use of OTC with excessive doses potentially leads to residue formation in animal products that can be harmful to consumers in the form of allergic reaction or resistance. This study aimed to detect OTC residues in broiler chicken meat, marketed in traditional markets and supermarkets in Depok, Bekasi, Bandung, Cilegon, Surakarta and Yogyakarta using indirect competitive enzyme-linked immunosorbent assay (icELISA) method. The analyses of 67 broiler meat samples showed only 1 (1.5%) sample was positive for the OTC residue at 86.1 ng/g which meant below the maximum residue limits of permissible OTC (100 ng/g). Nevertheless, a stricter regulation for the use of OTC in the poultry industry and the monitoring of its residue in chicken products prior to marketing is still necessary to avoid the adverse effects of the residue present in animal products.

<p>This present study was aimed to investigate the presence of aflatoxin M1 (AFM1) residue in fresh dairy milk collected from small dairyl farms. A total of 104 samples of fresh cow's milk were collected in Pengalengan-Bandung and Sukabumi (West Java province), and Tanggamus (Lampung province) in April and September 2012. All samples were analyzed by a high performance liquid chromatography and detected with fluorescence detector after extraction with organic solvents. Contamination of AFM1 was found on 1.96% (1/51) from the samples collected in April 2012 at concentration of 1.20 ng/L and 39.63% (21/53) from the samples collected in September 2012 at concentration of 1.20 ng/L 1.0 – 34.1 ng/L. Those positive samples were obtained from Pangalengan and Sukabumi, but none for those samples collected from Tanggamus both on collection time April nor September 2012. In those positive samples for AFM1, there is no sample contained AFM1 above the maximum level (ML) regulated in Indonesia (500 ng/L or 0.5 µg/L). Low contamination levels of AFB1 in the range of 0.38 to 6.64 µg/kg found in supplemental feed samples from the same sampling time and locations. The findings of AFM1 contamination in raw fresh milk from this study caused no harm to the consumers. However, regular monitoring on the presence of AFM1 in dairy milk and aflatoxin B1 (AFB1) in dairy cattle feed is necessary to ensure the protection of human health.</p>

Tetracycline residues are group of antibiotics found in animal products such as dairy milk. In this study, the occurrence of tetracycline residues in 45 dairy milk powder and 24 fresh dairy milk samples have been investigated. Tetracycline residue were extracted from milk samples using McIlvine buffer and purified with a solid phase extraction (SPE) C18 column. Residues were detected through HPLC-PDA at wavelengths of 355 and 368 nm. Three forms of tetracyclines were discovered in the 45 milk powder samples, namely; oxytetracycline (31.75 ng/g), tetracycline (28.02 ng/g) and chlortetracycline (10.55 ng/g). The results of 24 fresh dairy milk samples, on the other hand, revealed that only one form of tetracycline (i.e., oxytetracycline) was detected with concentrations ranging from 6.67 to 10.19 ng/g. Although the low incidence and concentration levels of tetracycline contamination in all samples analysed were not alarming, regular monitoring is recommended to avoid any negative public health consequences.

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