Determination of sex from tooth pulp tissue

Abstract

ObjectiveThis study was carried out to determine the reliability of sex determination from teeth pulp tissue.Patients and methodsThis study was carried on 60 maxillary and mandibular premolars and permanent molars (30 male teeth and 30 female teeth) which were indicated for extraction. The teeth were categorized into three groups of 20 each (10 from males and 10 from females).Group 1-pulp tissue from teeth examined immediately after extraction. Group 2- and Group 3-pulp tissue examined from teeth one and five month after extraction, respectively. Teeth was sectioned and pulpal cells were stained with quinacrine dihydrochloride. The cells were observed with fluorescent microscope for fluorescent body. Gender was determined by identification of Y chromosome fluorescence in dental pulp.ResultsFreshly extracted teeth and for those examined one month later, sensitivity, specificity, positive predictive value, negative predictive value, and efficiency were all 100%.ConclusionThe fluorescent Y body test is shown to be a reliable, simple, and cost-effective technique for gender identification in the immediate postmortem period up to one month.

Sex determination from dental pulp DNA among Egyptians

“…In this regard, many studies have used the dental pulp for sex determination (Veeraraghavan et al, 2010;Zapico & Ubelaker, 2013;Nogami et al, 2008;Nayar et al, 2014;Naik et al, 2012).…”

Pulp tissue in sex determination: A fluorescent microscopic study

“…[5] So, the present study considered the effect of caries on fluorescent body test as literature (when searched via PUBMED and MEDLINE) revealed lack of studies analyzing this fact.…”

“…In some cases, however, fingerprints are not available from the deceased, or ante-mortem prints cannot be obtained. [ 5 ] Sex determination from skull is highly unreliable although number of workers have described differences in the size of male and female teeth, such measurements seem unlikely to be used to determine the sex of an individual. [ 7 ] So, a technique has been developed, which may offer a solution whenever soft tissue remains can be recovered.…”

“…Tooth pulp is encased in a hard tissue casting, where it may be protected from detrimental effects of impact, trauma, and heat. [ 5 ] So, Casperson et al developed a technique using pulpal tissue stained with quinacrine mustard, specific for Y chromosome to determine sex of an individual. [ 6 ] In literature, there is scarcity of relevant studies that analyzed the effect of caries on viability of pulpal tissue in sex determination.…”

Dental Pulp Fibroblast and Sex Determination in Controlled Burial Conditions

“…One of the fundamental or key parameters to begin the process of forensic identification is sex estimation (Veeraraghavan et al, 2010), for which the use of genetic material is relevant (Iwamura et al, 2004). In the search of new, simple, quick and at lower cost methods, the observation of sexual chromatin or Barr Body has been used (Barr & Bertram, 1949), which are found in several types of somatic cells (Vera et al, 1986), as is present in some cellular elements of dental pulp (Suazo et al, 2011).…”