Aims:To determine and compare the reliability of pulp tissue in determination of sex and to analyze whether caries have any effect on fluorescent body test.Materials and Methods:This study was carried on 50 maxillary and mandibular teeth (25 male teeth and 25 female teeth), which were indicated for extraction. The teeth are categorized into 5 groups, 10 each (5 from males and 5 from females) on the basis of caries progression. The pulp cells are stained with quinacrine hydrochloride and observed with fluorescent microscope for fluorescent body. Gender is determined by identification of Y chromosome fluorescence in dental pulp.Results:Fluorescent bodies were found to be more in sound teeth in males as the caries increase the mean percentage of fluorescent bodies observed decreases in males. We also observed the fluorescent spots in females, and the value of the spot increases in female as the caries progresses, thereby giving false positive results in females.Conclusion:Sex determination by fluorescent staining of the Y chromosome is a reliable technique in teeth with healthy pulps or caries with enamel or up to half way of dentin. Teeth with caries involving pulp cannot be used for sex determination.

Background:Iron overload is a medical condition that occurs when too much of the mineral iron builds up inside the body and produces a toxic reaction. Thalassemia is a genetic disorder of hemoglobin synthesis, which requires regular blood transfusion therapy, and the lack of specific excretory pathways for iron in humans leads to iron overload in the body tissues. It is a major cause of morbidity and mortality in these patients. The estimation of iron levels in exfoliated buccal mucosal cells may provide a simple, noninvasive, and a safe procedure for estimating the iron overload by using the Perls’ Prussian blue stain.Methods:Smears were obtained from buccal mucosa of 40 randomly selected beta-thalassemia major patients and 40 healthy subjects as controls. Smears were stained with Perls’ Prussian blue method. Blood samples were taken for estimation of serum ferritin levels. Images of smears were analyzed using the software image J software version 1.47v and correlated with serum ferritin.Results:Perls’ positivity was observed in 87.5% of thalassemic patients with a positive correlation to serum ferritin levels.Conclusion:The use of exfoliative buccal mucosal cells for the evaluation of iron overloads in the body provides us with a diagnostic medium that is noninvasive, easy to collect, store, and transport, cost effective, and above all reliable.

Double lip is a rare anomaly that may be congenital or acquired and affects upper lip more commonly than the lower lip. This deformity consists of an accessory fold of redundant mucous membrane inside the vermilion border. It affects esthetics and may also interfere with speech and mastication. Simple surgical excision produces good functional and cosmetic results. We describe a case of congenital maxillary double lip along with a brief review of the literature and discuss the differential diagnosis. This anomaly is of more relevance in dentistry as a general dental practitioner is usually the first professional to recognize and establish the diagnosis.

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To compare the levels of salivary Interleukin 6 between oral potentially malignant disorders and oral squamous cell carcinoma. After ethical clearance and informed consent, the saliva samples were collected from 20 patients of OPMD’S, 20 patients of OSCC and 10 controls. Salivary IL-6 levels were detected by enzyme linked Immunosorbent essay and appropriate statistical analysis was done. Significant differences in IL-6 concentration between OSCC, OPMD’S and controls were noted. Salivary IL-6 levels were higher in OSCC. The results of  present study concluded that the level of salivary IL-6 in OSCC is significantly higher than oral potentially malignant disorders like OSMF and oral epithelial dysplasia and controls. This biomarker can be utilized as a major asset for early detection of cancer and can open a new horizon for treatment plans of targeted therapy.