Dental Pulp Fibroblast and Sex Determination in Controlled Burial Conditions

Introduction:Identifying Barr bodies' presence has a significant diagnostic value in multifaceted science disciplines. Testing Barr bodies was important in diagnosing infertility, a syndromic association such as Klinefelter and psychopathic disorders, and disorders of sex development (DSD). It also plays a role in cancer detection in the uterine cervix, identifying transplanted retinal pigment epithelium in porcine models. Identifying the gender of victims or criminals becomes a fundamental requirement in any forensic analysis of a crime scene. The current study hypothesizes identifying the gender using Barr body detection from collected contact lens samples and tries to establish disposed soft contact lenses to consider as evidence found at the crime scene.Methods: A total of 120 (60 males and 60 females) were included in the study; from each subject, contact lens and Buccal samples were collected using sterile wooden toothpicks and soft contact lenses after insertion and removal. Both the buccal and contact lens samples were built into two smears staining with Saffranine and Methylene blue stains. The smears underwent cytological assessment by two examiners using a binocular microscope at 40X. The details of findings were graded on a scale of 1 to 5 based on visualization of Barr bodies seen. Results:The mean rank and median grading scores for higher using saffranine among females across both the samples. The sensitivity is higher at 100% for both the stains among contact lens samples, and specificity is higher among buccal samples, 93% for saffranine and 90% for methylene blue. Conclusion:Overall, it is conspicuous that contact lenses can be considered as evidence found at the crime scene in identifying the gender using Barr body detection.

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“…accuracy by Papanicolaou stain [28,29]. In another study on buccal smears, methylene blue showed 88% accuracy, and H&E showed 80% accuracy [30].…”

Section: Discussionmentioning

confidence: 99%

Contact Lens as Evidence in Crime Scene - A Way to Identify Gender

Koduri¹,

Vataparthi²,

Bolleda³

et al. 2023

Act Sci Oph

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“…Hence the quantity of the pulp tissue was less compared to that usually retrieved from the permanent teeth. 9 However, many studies have shown that it is possible to determine the gender of an individual even if the quantity of DNA obtained from the pulp tissue was as less as 20-70 pg of good quality DNA. 10 According to Jacqueline B.…”

Section: Discussionmentioning

confidence: 99%

Gender Determination through Molecular Analysis of Pulp Tissue of Deciduous Teeth–A Study Using Polymerase Chain Reaction Technique

2020

IJPHRD

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Background: Accurate determination of gender from the skeletal remains has a significant role in the identification process. In conditions of extreme fragmentation, information from DNA plays a vital role in establishing the gender of the person and further contributes to personal identification.Method: Sixty sound and non-carious extracted deciduous teeth were grouped into three of 20 each. Group 1 was analyzed immediately after extraction. Group 2 and Group 3 were stored at room temperature for three months and Fifteen months respectively before subjecting them to PCR analysis. The X and Y chromosomes from each sample were amplified and compared with the actual gender of the person. Shapiro-Wilk test the independent sample t-test, paired t-test and the chi-square test were used to analyze the data obtained. Result:The mean DNA volume (mg/ml) obtained immediately after extraction was significantly more than that from the teeth stored at room temperature for 15 months.The PCR analysis did not show any significant difference between group 1 and group 3 (p=0.072). Statistically significant difference was observed, when the results of group 1 were compared with that of group 3. Conclusion:Gender could be conclusively determined from the samples analyzed immediately after extraction. The accuracy of gender determination decreased as the period of storage increased.

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“Sex Chromatin in Dental Pulp as an Indicator in Gender Identity”

Gottipati

David

2022

Global Journal for Research Analysis

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Background and Objectives: Identication means determination of individuality of a person. Identication is important for legal, humanitarian reasons, in solving criminal cases, problems of inheritance, funeral rites. The estimation of sex is one of the pillars of forensic identication. The tooth organ is the hardest organ in the human body, with a loose connective tissue of dental pulp situated within a rigid encasement of mineralized surrounding tissues. Sex can be determined by the study of X&Y chromosomes in the cells which are not undergoing active division. X chromatin in its inactivated form is present as a mass against the nuclear membrane in females is known as Barr body. Presence or absence of X chromosome can be studied from buccal smears, skin biopsy, blood, cartilage, hair root sheath, and tooth pulp. The present study was undertaken to identify and evaluate the presence of X chromatin in dental pulp as an indicator in gender identity. The study was performed on healthy premolars, from a total of Materials and Methods: 80 subjects inclusive of 40 teeth extracted from males and 40 teeth extracted from females after informed consent. The collected tooth specimens were divided in to two groups. Group I consisted of 20 teeth from males, 20 teeth from females. Group II consisted of 20 teeth from males, 20 teeth from females. Group I premolars were immediately preserved in 10% formalin solution for upto 7 days. Group II premolars were buried in mud at room temperature for 4 months. The dental pulp tissues were conventionally extirpated and stained with hematoxylin & eosin stain. All the blinded sections of the dental pulp were observed for X chromatin/Barr bodies systematically using an Olympus BX53 microscope with a 100x magnication. I Results: n group I, Dental pulp was positive for X chromatin in 100% females for up to 7days with 100% accuracy. In group II, Dental pulp was positive for X chromatin in 45% of females after 4 months with 45% accuracy. The accuracy of identifying X chromatin in dental pulp diminished over a period of time. Dental pulp was negative for X chromatin in teeth of 100% males both at 7days (Group I) & 4 months (Group II). The identication of X chromatin in dental pulp as a gender deter Conclusion: minant can be ascertained with high reliability within 7 days of time. Whereas the reliability of identifying X chromatin in dental pulp as a gender determinant decreased in the course of time. Consequently, we can recommend identication of X chromatin in dental pulp as an indicator in gender identity within a short period of time.

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Dental Pulp Fibroblast and Sex Determination in Controlled Burial Conditions

Cited by 4 publications

References 13 publications

Contact Lens as Evidence in Crime Scene - A Way to Identify Gender

Contact Lens as Evidence in Crime Scene - A Way to Identify Gender

Gender Determination through Molecular Analysis of Pulp Tissue of Deciduous Teeth–A Study Using Polymerase Chain Reaction Technique

“Sex Chromatin in Dental Pulp as an Indicator in Gender Identity”

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