SANDOVAL, C.; NUÑEZ, M. & ROA, I.Dental pulp fibroblast and sex determination in controlled burial conditions. Int. J. Morphol., 32(2):537-541, 2014. SUMMARY:The need to identify bodies that are found as a result of disappearances with a diversity of causes, illegal burials and massive disasters, represent a wide percentage of dentistry practice on forensic research. The following study determined the performance of Barr Body Test, in fibroblasts of healthy teeth, under different conditions of burial (in vitro) with variations in pH, humidity and salinity in terms of general accuracy and sensitivity for men and women. Analyzed sample considered 47 dental pulps, taken from teeth under burial conditions during a period of a month. From dental pulps samples, 265 histological cuts valid for this study, were obtained, which were observed with an optical microscope under conventional H/E staining. Results showed a 98.9% of well-diagnosed cases, which correspond to the overall accuracy of the method. Sensitivity for men was 97.5% and 100% for women, over the analyzed sample. In low humidity conditions, 3 samples of badly diagnosed cases in men were observed, with a group accuracy of a 90%, with a sensitivity of 25% for men and 100% for women. The present study establishes that based on these results, the performance of Barr Body Test in fibroblasts, proposed for healthy pulp teeth, is not affected by burial conditions in terms of pH (acid-alkaline), salinity (high-low) and high humidity.
RESUMEN: Las ocurrencias de lesiones en la región oro-maxilofacial adquieren importancia debido a su complicada anatomía y fisiología, pudiendo resultar en deformidades faciales, adquiriendo interés cuando son causadas por un tercero, pudiendo traer repercusiones legales. El objetivo fue realizar un estudio transversal con el fin de estimar frecuencia y tipificación de lesiones oro-maxilofaciales que requirieron peritaje forense en el Servicio Médico Legal de Curicó, Chile. Se recopilaron datos encriptados de 79 fichas de pacientes entre 17-88 años que realizaron su constatación de lesiones en Servicio Médico Legal de Curicó, Chile, en el lapsus de un año. La frecuencia de lesiones con peritaje forense en la región oro-maxilofacial fue de un 25,82 %, provocada principalmente por mecanismo físico. En su mayoría efectuados a individuos del sexo masculino, con un rango etario de entre 20 a 40 años. La violencia interpersonal fue observada como el agente causal más frecuente de lesiones, seguida por accidentes de tránsito. Los sujetos periciados por violencia intrafamiliar, fueron en su totalidad mujeres. Las lesiones más recurrentes fueron fractura y contusión, dentro de ellas encontramos a fractura nasal como la más frecuente, seguida de herida contusa, herida por instrumento cortante, fractura maxilar y por último fractura dental. De la totalidad de las lesiones en estudio solo un tercio estuvieron confinadas exclusivamente en el territorio oro-maxilofacial.
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