Determination of ractopamine in pig hair using liquid chromatography with tandem mass spectrometric detection

Wu, Junlin; Liu, Xiaoyun; Peng, Yunping

doi:10.1016/j.vascn.2014.02.001

Cited by 18 publications

(7 citation statements)

References 19 publications

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“…These methods include liquid chromatography (Chang, Chou, & Hwang, 2005;Meng et al, 2012;Turberg, Macy, Lewis, & Coleman, 1994), gas chromatography (Bocca, Fiori, Cartoni, & Brambilla, 2003), liquid chromatography tandem mass spectrometry (LC-MS/MS) (Shishani, Chai, Jamokha, Aznar, & Hoffman, 2003;Wu, Liu, & Peng, 2014), gas chromatography-mass spectrometry (Montrade, Lebizec, Monteau, & Andre, 1995;Wang, Li, Zhang, & Shen, 2006), enzyme-linked immunoassay (Hahnau & Julicher, 1996;Harkins, Woods, Lehner, Fisher, & Tobin, 2001;Pinheiro et al, 2008), colloidal gold test strip (Khamta, Pattarawarapan, Nangola, & Tayapiwatana, 2009;Zhang et al, 2009), surface plasmon resonance immunosensors (Traynor, Crooks, Bowers, & Elliott, 2003), and electrochemical immunosensors (He, Wang, Zhang, & Yang, 2009). Table 1 Chemical formula, accurate mass, retention time and characteristic fragment ions of 32 b-agonists.…”

Section: Introductionmentioning

confidence: 99%

Broad screening and identification of β-agonists in feed and animal body fluid and tissues using ultra-high performance liquid chromatography-quadrupole-orbitrap high resolution mass spectrometry combined with spectra library search

Cao

et al. 2016

Food Chemistry

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Section: Introductionmentioning

confidence: 99%

Broad screening and identification of β-agonists in feed and animal body fluid and tissues using ultra-high performance liquid chromatography-quadrupole-orbitrap high resolution mass spectrometry combined with spectra library search

Cao

et al. 2016

Food Chemistry

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“…To determine the ractopamine levels in plasma collected from healthy goats and sheep treated with this agent, we initially utilized the HPLC assay described by other researchers without modification (9,15,16). However, we were unable to reproduce the reported assay so we developed a simpler assay using an isocratic mobile phase.…”

Section: Discussionmentioning

confidence: 99%

Assessment of the withdrawal period for ractopamine hydrochloride in the goat and sheep

Lazuardi¹,

Hermanto²,

Restiadi³

2020

IJVS

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Ractopamine hydrochloride often used as a bronchodilator, but its β-adrenergic agonist effects on un-striated muscle and its withdrawal time have not been assessed for Etawah goats and sheep. The aim of this study was to determine the safe time to slaughter goats and sheep post-treatment with ractopamine. Five clinically healthy adult goats and sheep (20 kg body weight) were treated with a single dose of ractopamine (1 mg, intravenously). Whole blood was sampled from the jugular vein at 120 min, 180 min and 300 min post-treatment. Ractopamine as a veterinary drug was analysed using HPLC at wavelength 225 nm.

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“…Each method has unique extraction technology, clean-up step or derivative procedure for increasing the efficiency and sensitivity of β-agonists detection in different food producing species. However, analytical methods for β-agonists in hair samples are few [ 19 ] but other studies have used pig hair samples to determine drug residues [ 15 , 19 ].…”

Section: Introductionmentioning

confidence: 99%

Determination of ractopamine and salbutamol in pig hair by liquid chromatography tandem mass spectrometry

Chang

Tsai

2018

Journal of Food and Drug Analysis

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A liquid chromatography tandem mass spectrometric method was developed for the determination of two β-agonists (ractopamine and salbutamol) in pig hair samples. An isotope of ractopamine-d5 or salbutamol-d6 as an internal standard was used to carry out quantitative analysis. Concentrated sodium hydroxide was used to pretreat hair samples and then purified by the solid phase extraction (SPE) procedure. The extracted solution was evaporated and reconstituted for injection in the instrument with electrospray ionization (ESI) operating in a positive multiple-reaction-monitoring (MRM) mode. Ractopamine and salbutamol separation were performed on C18 analytical column under gradient condition. The internal standard calibration curve was linear in the range of concentration from 0.5 to 100 ng mL (R > 0.995). Recoveries of this method estimated at three spiked concentrations of 100, 250 and 500 ng mL in pig hair samples, were 79-82% for ractopamine and 77-96% for salbutamol. The corresponding inter-day and intra-day precisions expressed as relative standard deviation (RSD %) were 3.8-6.4% and 3.8-8.6%, respectively. The analytical time for one sample was 8 min. The detection limit of this method was 0.6 and 8.3 ng mL for ractopamine and salbutamol, respectively. This developed method can be applied for monitoring the use of the β-agonists salbutamol and ractopamine in swine feed incurred pig hair.

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Determination of ractopamine in pig hair using liquid chromatography with tandem mass spectrometric detection

Cited by 18 publications

References 19 publications

Broad screening and identification of β-agonists in feed and animal body fluid and tissues using ultra-high performance liquid chromatography-quadrupole-orbitrap high resolution mass spectrometry combined with spectra library search

Broad screening and identification of β-agonists in feed and animal body fluid and tissues using ultra-high performance liquid chromatography-quadrupole-orbitrap high resolution mass spectrometry combined with spectra library search

Assessment of the withdrawal period for ractopamine hydrochloride in the goat and sheep

Determination of ractopamine and salbutamol in pig hair by liquid chromatography tandem mass spectrometry

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