Broad screening and identification of β-agonists in feed and animal body fluid and tissues using ultra-high performance liquid chromatography-quadrupole-orbitrap high resolution mass spectrometry combined with spectra library search

Li, Tingting; Cao, Jingjing; Li, Zhen; Xian, Wang; He, Pingli

doi:10.1016/j.foodchem.2015.06.104

Cited by 51 publications

(21 citation statements)

References 31 publications

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“…Several research groups evaluated MS settings during DDA mode in order to examine their significance in the experimental outcome and optimize instrument performance for a successful analysis. In terms of MS parameters, the effect of dynamic exclusion, signal thresholds setting to trigger an MS/MS event, mass resolving power, ion injection time, number of MS/MS, monoisotopic precursor selection, preview mode for FTMS scan, normalized collision energy, automatic gain control (AGC) target for MS and MS/MS scans, and number of microscans have been evaluated and discussed, aiming to examine the significance and effect of instrument settings on identification rates. These reports revealed that MS settings influence identification rates and improved results can be obtained by optimizing instrument performance .…”

Section: Resultsmentioning

confidence: 99%

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Optimization of mass spectrometry acquisition parameters for determination of polycarbonate additives, degradation products, and colorants migrating from food contact materials to chocolate

et al. 2017

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The interest towards "substances of emerging concerns" referred to objects intended to come into contact with food is recently growing. Such substances can be found in traces in simulants and in food products put in contact with plastic materials. In this context, it is important to set up analytical systems characterized by high sensitivity and to improve detection parameters to enhance signals. This work was aimed at optimizing a method based on UHPLC coupled to high resolution mass spectrometry to quantify the most common plastic additives, and able to detect the presence of polymers degradation products and coloring agents migrating from plastic re-usable containers. The optimization of mass spectrometric parameter settings for quantitative analysis of additives has been achieved by a chemometric approach, using a full factorial and d-optimal experimental designs, allowing to evaluate possible interactions between the investigated parameters. Results showed that the optimized method was characterized by improved features in terms of sensitivity respect to existing methods and was successfully applied to the analysis of a complex model food system such as chocolate put in contact with 14 polycarbonate tableware samples. A new procedure for sample pre-treatment was carried out and validated, showing high reliability. Results reported, for the first time, the presence of several molecules migrating to chocolate, in particular belonging to plastic additives, such Cyasorb UV5411, Tinuvin 234, Uvitex OB, and oligomers, whose amount was found to be correlated to age and degree of damage of the containers.

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Section: Resultsmentioning

confidence: 99%

“…Until now, several studies have been reported application of the hybrid instrument Q-Exactive in the field of food quality and safety. [12][13][14][15][16] However, in literature, there are no data about extraction methods of most used plastic additives and BPA from complex food.…”

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confidence: 99%

Optimization of mass spectrometry acquisition parameters for determination of polycarbonate additives, degradation products, and colorants migrating from food contact materials to chocolate

et al. 2017

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“…However, the illegal use of these compounds continues to be widely reported . Over the past few decades, methods have been developed to monitor the illegal use of these compounds in both humans and animals . It is generally accepted that chemical testing of biological fluids is the most objective approach to diagnose drug use.…”

Section: Introductionmentioning

confidence: 99%

“…1,2 Over the past few decades, methods have been developed to monitor the illegal use of these compounds in both humans and animals. [3][4][5][6][7][8][9][10][11][12][13] It is generally accepted that chemical testing of biological fluids is the most objective approach to diagnose drug use. The presence of a drug analyte in a biological specimen can be used to document exposure to that drug.…”

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confidence: 99%

The use of hair as a long‐term indicator of low‐dose β₂ agonist treatments in cattle: Implications for growth‐promoting purposes monitoring

Liu

Tang

Long

et al. 2019

Drug Testing and Analysis

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The objective of this study was to assess the feasibility of using hair as a long‐term indicator of cocktail (low‐dose β2 agonists) treatments in cattle. Six male Simmental cattle were treated with a mixture of low‐dose clenbuterol, ractopamine, and salbutamol at dosages of 5.3, 223.3, and 50.0 μg/kg, respectively. The trial lasted for 112 days and included 28 days of treatment and 84 days of withdrawal. Plasma and urine samples taken during the treatment period contained the highest residues, with maximum concentrations of clenbuterol, ractopamine, and salbutamol in plasma of 1.49 ng/mL (Day 21), 43.78 (Day 14) ng/mL, and 8.07 ng/mL (Day 7), respectively, and in urine of 62.40 ng/mL (Day 28), 3995.77 ng/mL (Day 28), and 503.72 ng/mL (Day 1), respectively. On day 42 of withdrawal, the residues of all three β2 agonists in plasma were below the limit of quantification (LOQ; 0.3 ng/mL for clenbuterol, and 0.5 ng/mL for ractopamine and salbutamol), and in urine samples were below or near the LOQ (the highest being ractopamine at 1.10 ng/mL). The highest concentrations of clenbuterol, ractopamine, and salbutamol in hair were 88.36, 1351.92, and 100.58 ng/g, respectively, on day 14 of withdrawal; and the residues were long‐lasting, with 7.64, 28.55, and 8.77 ng/g, respectively, on day 84 of withdrawal. The results of this study demonstrate that hair could be utilized as a long‐term indicator of the use of a combination of low‐dose β2 agonists in cattle, which could have implications for growth‐promoting purposes monitoring.

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“…In this work, the detected brombuterol (Brom, 4‐amino‐3,5‐dibromo‐α[(tert.‐butylamino) methyl] benzyl alcohol) is a kind of β‐AA . Brom has been used as the growth promoting agent and feed additive for cattle, sheep, poultry and other livestock on account of it significantly improved the carcass lean percentage, weight gain and feed conversion rate . β‐AA residues in food from food‐producing animals may pose a risk on human health, such as headache, muscular pain, cardiac palpitation, dizziness, nausea, vomiting, fever and even death .…”

Section: Introductionmentioning

confidence: 99%

Signal Amplification Strategy for Highly Sensitive Detecting Brombuterol with Electrochemiluminescent Immunoassay by Using CdSe QDs as Label and Gold Nanoparticle as Substrate

et al. 2016

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1Introduction b-adrenergic agonists (b-AA), commonlyk nowna sc lenbuterol, is akind of drugthat can significantly reduce adipose tissue accretionb ythe combinede ffects of inhibiting fatty acid biosynthesis and concurrently stimulating triacylglycerol( TAG) lipolysis,o rh ydrolysis [1].I nt his work, the detected brombuterol ( Brom, methyl] benzyl alcohol) is ak ind of b-AA [2].B rom has been used as the growth promoting agent and feed additive for cattle,s heep,p oultry and otherl ivestock on account of it significantly improved the carcass lean percentage,w eight gain and feed conversion rate [3]. b-AA residues in food from foodproducing animals may pose ar isk on human health, such as headache,m uscular pain, cardiac palpitation, dizziness, nausea, vomiting, fever and even death [4].C hina and some other countries have listed the b-AA as directory of prohibited drugs for animals.S ome illegal businessmen are optingt or educe their input costs whicha re primarily made up of feed costsi ns earch for ab etter profit margin [5,6].B rom is used as feed additive,t herefore the means for detecting residues in food and feed are badly in need of enhancement.At present, the b-AA detectionm ethodsa re mainly containing high performance liquid chromatography (HPLC) [ 7],m agnetic solid-phase extraction method (MSPE)[ 8],u ltra-highp erformance liquid chromatography (UHPLC/MS/MS) [9,10],l iquid chromatographymass spectrometry (LC-MS) [11],e nzyme-linked immu-nosorbent assay (ELISA) [ 2,12],g as chromatographymasss pectrometry (GC-MS) [13],e tc. With the disadvantages of sample treatment for al ong time,c umbersome detectionp rocess and difficult to operate,e xpensivei nstruments,p henomenon of false positives, the practical applications are limited. In this paper, electrochemical immunosensor prepared by competition immune method has been ak ind of practicalv alue and scientific value of detectingB rom.Electrochemiluminescence (ECL),a lso known as electric chemical luminescence,i sakind of analytical techniques combining the characteristics of the controllability of electrochemical potential and the high sensitivity of the luminescence analysis, which has become one of the mostp owerful analysis techniques [14][15][16].I nr ecent years,e lectrochemicall uminescence immunoassay (ECLIA) as ap owerful bioanalysis technique is composedo fE CL and immunoassayw ith values of specific identification, stability,s implified optical setup, high sen-Abstract:Asignal amplifications trategy for electrochemiluminescence (ECL)i mmunosensor was designed based on gold nanoparticles (AuNPs) and L-cysteine capped CdSe QDs for highly sensitive detection of brombuterol( Brom) in the presence of K 2 S 2 O 8 as the co-reactant. Therein, AuNPs as the substrate can simplify the experiment process, accelerate the electron transfer rate, and enhance the ECL signal. In the presence of Brom standard solution, coating antigen immobilized on the electrode surface via AuNPs would compete with Brom for the limited binding sites of the CdSeQ Ds labeled Bro...

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Broad screening and identification of β-agonists in feed and animal body fluid and tissues using ultra-high performance liquid chromatography-quadrupole-orbitrap high resolution mass spectrometry combined with spectra library search

Cited by 51 publications

References 31 publications

Optimization of mass spectrometry acquisition parameters for determination of polycarbonate additives, degradation products, and colorants migrating from food contact materials to chocolate

Optimization of mass spectrometry acquisition parameters for determination of polycarbonate additives, degradation products, and colorants migrating from food contact materials to chocolate

The use of hair as a long‐term indicator of low‐dose β₂ agonist treatments in cattle: Implications for growth‐promoting purposes monitoring

Signal Amplification Strategy for Highly Sensitive Detecting Brombuterol with Electrochemiluminescent Immunoassay by Using CdSe QDs as Label and Gold Nanoparticle as Substrate

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Broad screening and identification of β-agonists in feed and animal body fluid and tissues using ultra-high performance liquid chromatography-quadrupole-orbitrap high resolution mass spectrometry combined with spectra library search

Cited by 51 publications

References 31 publications

Optimization of mass spectrometry acquisition parameters for determination of polycarbonate additives, degradation products, and colorants migrating from food contact materials to chocolate

Optimization of mass spectrometry acquisition parameters for determination of polycarbonate additives, degradation products, and colorants migrating from food contact materials to chocolate

The use of hair as a long‐term indicator of low‐dose β2 agonist treatments in cattle: Implications for growth‐promoting purposes monitoring

Signal Amplification Strategy for Highly Sensitive Detecting Brombuterol with Electrochemiluminescent Immunoassay by Using CdSe QDs as Label and Gold Nanoparticle as Substrate

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The use of hair as a long‐term indicator of low‐dose β₂ agonist treatments in cattle: Implications for growth‐promoting purposes monitoring