Determination of ochratoxin a in beer by high-performance liquid chromatography

Degelmann, Petra; Becker, M.; Herderich, Markus; Humpf, Hans‐Ulrich

doi:10.1007/bf02467756

Cited by 33 publications

(17 citation statements)

References 16 publications

(13 reference statements)

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“…The incidence found in Brazilian beer in the present survey (4.8%) is therefore low. The levels found, on the other hand are higher then the levels reported by other countries by various orders of magnitude 12,13,21,26,27,35,47,52,53 .…”

Section: Resultscontrasting

confidence: 56%

“…No positive results for OTA presence were detected in any of the beer samples. German beers (35 samples) were analyzed by DEGELMANN et al 13 and OTA levels in the range of 0.1-0.2 ng.mL -1 were found in 9 beer samples, 21 samples contained trace amounts of OTA (<0.1 ng.mL -1 ) and in 5 samples no OTA was detectable. SCOTT and KANHERE 35 found traces of OTA in 26 out of 46 samples of Canadian and imported beers.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Fumonisin B1 and ochratoxin A in beers made in Brazil

Kawashima¹,

Vieira²,

Soares³

2007

Ciênc. Tecnol. Aliment.

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Samples of beer made in Brazil were analyzed for the presence of fumonisin B 1 (FB 1 ) and ochratoxin A (OTA). FB 1 was searched for in 58 beer samples from 30 plants located in nine states. The samples were concentrated and cleaned up with strong ion exchange column, derivatized with OPA and analyzed by HPLC with fluorescence detection. The limit of detection was 0.26 ng.mL -1 and the average recovery was 98%. Twenty-five samples contained FB 1 ranging from 1 to 40 ng.mL -1 . Beer (123 samples) from 36 plants located in 5 states were analyzed for OTA by means of immunoaffinity column cleanup followed by liquid chromatography associated with fluorescence. The detection limit was 0.1 ng.mL -1 and the average recovery was 92%. Five samples contained OTA in concentrations from 1 to 18 ng.mL -1 . The results indicate that FB 1 and OTA contamination in Brazilian beer is not geographically limited and that beer does not contribute significantly to FB 1 intake by consumers. In the case of regular high ingestion, beer could contribute sizably to OTA, intake although still below the maximum considered tolerable for the toxin. Keywords: mycotoxins; fumonisin B 1 ; ochratoxin A; beer. ResumoA presença de fumonisina B 1 (FB 1 ) e de ocratoxina A (OTA) foi investigada em amostras de cerveja fabricada no Brasil. FB 1 foi pesquisada em 58 amostras de cerveja provenientes de 30 fábricas localizadas em nove Estados. As amostras foram concentradas, e a toxina isolada através de coluna de troca iônica forte, derivação com OPA e análise por CLAE com fluorescência associada. O limite de detecção foi 0,26 ng.mL -1 e a recuperação média foi de 98%. Vinte e cinco amostras continham FB 1 em concentrações de 1 a 40 ng.mL -1 . Cerveja (123 amostras) proveniente de 36 fábricas localizadas em 5 Estados foi analisada para OTA através de coluna de imunoafinidade seguida de CLAE com detector de fluorescência. O limite de detecção foi 0,1 ng.mL -1 e a recuperação média foi de 92%. Cinco amostras continham OTA em concentrações de 1 a 18 ng.mL -1 . Os resultados indicam que a contaminação da cerveja brasileira por FB 1 e por OTA não é geograficamente limitada e que não contribui significativamente para a ingestão de FB 1 por consumidores. Por outro lado, no caso de consumo alto e regular, esta pode contribuir substancialmente na ingestão de OTA, porém ainda abaixo do máximo considerado tolerável para a toxina. Palavras-chave: micotoxinas; fumonisina B 1 ; ocratoxina A; cerveja.

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Section: Resultscontrasting

confidence: 56%

Section: Introductionmentioning

confidence: 99%

Fumonisin B1 and ochratoxin A in beers made in Brazil

Kawashima¹,

Vieira²,

Soares³

2007

Ciênc. Tecnol. Aliment.

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“…LEGARDA & BURDASPAL [13] verified the presence of OTA in 37 of 38 Spanish beers (97.4%) with mean value of 24pg/mL. DEGELMANN et al [4] verified in 35 samples of German beers OTA levels in the range of 100 200ng/mL in 9 beer samples, 21 samples contained trace amounts of OTA and in 5 samples no OTA was detectable. In 2000, VISCONTI, PASCALE & CENTONZE [31] found OTA in different brands of beer produced in Europe and Italy.…”

Section: -Results and Discussionmentioning

confidence: 99%

“…The methods currently used are often based on reversed phase high performance liquid chromatography with fluorescence detector [4,12,13,18,23,31].…”

Section: -Introductionmentioning

confidence: 99%

Ochratoxin A determination in beer by immunoaffinity column clean-up and high-performance liquid chromatography

Prado¹,

Oliveira²,

Carvalho³

et al. 2003

Ciênc. Tecnol. Aliment.

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“…Thus, the method can be a good confirmatory test for samples already turned out to be positive with fluorescence detection. The same procedure was applied by the same group (Degelmann et al, 1999) for the detection of OTA in beer. Again, the LC/MS/MS method was used mainly for confirmatory purposes, after a positive sample was detected by LC/FLD.…”

Section: A Liquid Chromatography/mass Spectrometry Determination Of mentioning

confidence: 99%

Recent advances in mycotoxin determination in food and feed by hyphenated chromatographic techniques/mass spectrometry

Sforza

Dall’Asta

Marchelli

2005

Mass Spectrometry Reviews

195

104

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Mycotoxins are fungal toxins produced by molds, which occur universally in food and feed derivatives, and are produced under certain environmental conditions in the field before harvest, post‐harvest, during storage, processing, and feeding. Mycotoxin contamination is one of the most relevant and worrisome problem concerning food and feed safety because it can cause a variety of toxic acute and chronic effects in human and animals. In this review we report the use of mass spectrometry in connection with chromatographic techniques for mycotoxin determination by considering separately the most diffuse class of mycotoxins: patulin, aflatoxins, ochratoxin A, zearalenone, trichothecenes, and fumonisins. Although the selectivity of mass spectrometry is unchallenged if compared to common GC and LC detection methods, accuracy, precision, and sensitivity may be extremely variable concerning the different mycotoxins, matrices, and instruments. The sensitivity issue may be a real problem in the case of LC/MS, where the response can be very different for the different ionization techniques (ESI, APCI, APPI). Therefore, when other detection methods (such as fluorescence or UV absorbance) can be used for the quantitative determination, LC/MS appears to be only an outstanding confirmatory technique. In contrast, when the toxins are not volatile and do not bear suitable chromophores or fluorophores, LC/MS appears to be the unique method to perform quantitative and qualitative analyses without requiring any derivatization procedure. The problem of exact quantitative determination in GC/MS and LC/MS methods is particularly important for mycotoxin determination in food, given the high variability of the matrices, and can be solved only by the use of isotopically labeled internal standards or by the use of ionization interfaces able to lower matrix effects and ion suppressions. When the problems linked to inconstant ionization and matrix effects will be solved, only MS detectors will allow to simplify more and more the sample preparation procedures and to avoid clean‐up procedures, making feasible low‐cost, high‐throughput determination of mycotoxins in many different food matrices. © 2005 Wiley Periodicals, Inc.

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Determination of ochratoxin a in beer by high-performance liquid chromatography

Cited by 33 publications

References 16 publications

Fumonisin B1 and ochratoxin A in beers made in Brazil

Fumonisin B1 and ochratoxin A in beers made in Brazil

Ochratoxin A determination in beer by immunoaffinity column clean-up and high-performance liquid chromatography

Recent advances in mycotoxin determination in food and feed by hyphenated chromatographic techniques/mass spectrometry

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