Determination of nitrate and bromide in foodstuffs by high-performance liquid chromatography

Leuenberger, Urs A.; Gauch, R.; Rieder, Konrad; Baumgartner, E.

doi:10.1016/s0021-9673(00)91832-6

Cited by 54 publications

(7 citation statements)

References 5 publications

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“…They include anion detection by conductimetry [8,9], indirect fluorimetry [10] or u.v. absorption [11][12][13]. On the basis of the latter we have developed a simple and sensitive method to determine low intracellular pools of nitrate in unicellular cyanobacteria (blue-green algae).…”

Section: Introductionmentioning

confidence: 99%

Determination of intracellular nitrate

Romero

Lara

Guerrero

1989

Biochemical Journal

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A sensitive procedure has been developed for the determination of intracellular nitrate. The method includes: (i) preparation of cell lysates in 2 M-H3PO4 after separation of cells from the outer medium by rapid centrifugation through a layer of silicone oil, and (ii) subsequent nitrate analysis by ion-exchange h.p.l.c. with, as mobile phase, a solution containing 50 mM-H3PO4 and 2% (v/v) tetrahydrofuran, adjusted to pH 1.9 with NaOH. The determination of nitrate is subjected to interference by chloride and sulphate when present in the samples at high concentrations. Nitrite also interferes, but it is easily eliminated by treatment of the samples with sulphamic acid. The method has been successfully applied to the study of nitrate transport in the unicellular cyanobacterium Anacystis nidulans.

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Section: Introductionmentioning

confidence: 99%

Determination of intracellular nitrate

Romero

Lara

Guerrero

1989

Biochemical Journal

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“…Our method of nitrate determination by ion-chromatography was based on published experience with the direct photometric nitrate detection in the eluate using light absorption at 200-210 nm [9, lo], and with the chromatographic system stationary-mobile phases [7]. However, we used a sorbent with chemically bonded diethylaminoethyl group to an organic polymer matrix as the stationary phase, the decreased concentration of H,PO,in the mobile phase and a lower pH value (2.2).…”

Section: Resultsmentioning

confidence: 99%

“…The use of this method for nitrate determination in different food products has already been reported (e.g. in vegetable [6], in cheese, whey and vegetable [7]). …”

Section: Introductionmentioning

confidence: 94%

Determination of Nitrates in Milk by Ion‐Chromatography

Vláčil

Vinš

1985

Nahrung

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A method is described for the determination of nitrates in cow milk, human milk, milk powder or milk-based infant formulae using liquid chromatography on Spheron DEAE and a direct photometric detection (205 nm). The influence of removing proteins by precipitation with Carrez reagent on the accuracy of determination was studied. The proposed method gives identical results with the reference method (photometry after reduction of nitrate to nitrite) but is more rapid. Its limit of determination is 0.5 mg NO3-/l of milk, its reproducibility is 4% (relative standard deviation).

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“…Nitrite concentration proportional to OD 540 was determined using a microplate reader (BIO‐TEK, EL 311s), with reference to a standard curve. The simultaneous determination of nitrite and nitrate ions was performed using an HPLC method described previously including anion exchange chromatography and photometric detection at 200 nm [6, 7]. Prior to sample injection, remaining sydnonimines and their degradation products, which interfered with the measurement of nitrite/nitrate, were removed by solid phase extraction using cation exchange columns (Adsorbex SCX, Merck, Darmstadt, FRG).…”

Section: Methodsmentioning

confidence: 99%

Photoactivation of the nitric oxide donor SIN‐1

et al. 1997

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This study investigates whether nitric oxide (NO) release from linsidomine (SIN-1), the active metabolite of the antianginal drug molsidomine, is light sensitive. A 9-h irradiation with polychromatic visible light increased the formation of nitrite (NO index metabolite) from SIN-1 (1 mM) by 61% as compared to control samples incubated in the dark. Under the same conditions (9-h irradiation) the concentration of oxygen decreased to 2% of control. However, oxygen consumption was substantially reduced when light was excluded (35% recovery after 9 h). These results show that irradiation with visible light markedly enhances the oxygen-dependent NO release from SIN-1. Thus, sydnonimines appear to be suitable model compounds for the development of photoactivatable NO donors.

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Determination of nitrate and bromide in foodstuffs by high-performance liquid chromatography

Cited by 54 publications

References 5 publications

Determination of intracellular nitrate

Determination of intracellular nitrate

Determination of Nitrates in Milk by Ion‐Chromatography

Photoactivation of the nitric oxide donor SIN‐1

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