Photoactivation of the nitric oxide donor SIN‐1

Abstract

This study investigates whether nitric oxide (NO) release from linsidomine (SIN-1), the active metabolite of the antianginal drug molsidomine, is light sensitive. A 9-h irradiation with polychromatic visible light increased the formation of nitrite (NO index metabolite) from SIN-1 (1 mM) by 61% as compared to control samples incubated in the dark. Under the same conditions (9-h irradiation) the concentration of oxygen decreased to 2% of control. However, oxygen consumption was substantially reduced when light was excluded (35% recovery after 9 h). These results show that irradiation with visible light markedly enhances the oxygen-dependent NO release from SIN-1. Thus, sydnonimines appear to be suitable model compounds for the development of photoactivatable NO donors.

Stimulatory and period-specific effect of nitric oxide on in vitro caulogenesis in Albizzia lebbeck (L.) Benth.

“…Although B5 basal medium, used in the present study, contains about 27 mM of nitrate in the form of ammonium and potassium nitrate, the observed stimulatory effect of NO donor could have been due to a negligible increase in the concentrations NO 2 -and NO 3 -, two stable metabolites of NO (Ullrich et al 1997). This remote possibility was ruled out as neither of NaNO 2 and NaNO 3, provided individually at 4 lM, had any effect on the caulogenic response (Table 2).…”

Nitric oxide promotes in vitro organogenesis in Linum usitatissimum L.

“…To rule out the possibility that the observed promotory effects of NO donors were due to NO À 2 and NO À 3 ; stable metabolites of NO (Ullrich et al 1997;Beligni and Lamattina 1999), the effect of 5 lM NaNO 2 and 5 lM NaNO 3 was also studied. Although MS basal medium, used in the present study, contains about 40 mM (40,000 lM) of nitrate, an additional 5 lM of NO À 2 or NO À 3 ; was supplemented to rule out the remote possibility of the observed increase in response due to this negligible rise in nitrate or nitrite concentration.…”

Analytical Chemistry of Nitric Oxide

“…Rather, we point the interested reader to a recent review describing applications of the Griess assay for elucidating the mechanisms of NO’s action in physiology (38) and to select research articles in which the Griess assay has been used to measure nitrite as a proxy for NO in urine (43), cerebrospinal fluid (44), plasma (45), serum (46), and blood (47). The Griess assay has also been used to quantify solution NO release from NO donors such as linsidomine (SIN-1) (48), sodium nitroprusside (49), poly(ethylene glycol)-lysine dendrimers (50), and self-assembling diazeniumdiolate-based nanofiber gels (51). …”