Determination of hypericin and pseudohypericin in pharmaceutical preparations by liquid chromatography with fluorescence detection

Quality control of Hypericum perforatum L. analytical challenges and recent progress

“…However, hypericin derivatives are not detectable with this method, contrary to FLD and DAD . Hypericin and pseudohypericin were quantified by fluorescence detection from the herbal matrix with a greater degree of specificity than HPLC‐UV and comparable sensitivity to some LC‐MS methods, with a limit of quantification of 0.18 ng …”

“…[81] Hypericin and pseudohypericin were quantified by fluorescence detection from the herbal matrix with a greater degree of specificity than HPLC-UV and comparable sensitivity to some LC-MS methods, with a limit of quantification of 0.18 ng. [82] Beside the pharmacologically active constituents, SJW contains a wide variety of other components that may play role in the clinical effect by influencing the bioavailability of pharmacophores. Therefore, determination of flavonoid content is the focus of several methods.…”

Separation methods of quinonoid constituents of plants used in Oriental traditional medicines

“…Fluorescence detection of hypericin and pseduohypericin is shown in Fig. 1d [181]. Recently, Schmidt [38] carried out the identification of naphthodianthrones from the plant extract by HPLC combined with on-line, pre-column photochemical conversion of the light-sensitive proto-forms of hypericin and pseudohypericin, followed by PDA detection.…”

Development of a high-performance liquid chromatographic method with electrochemical detection for the determination of hyperforin

“…The so obtained six calibration curves were used to calculate a mean calibration graph. The limit of quantification was defined as that lowest concentration where an accuracy better than 20.0% was achieved [26].…”