Development of a high-performance liquid chromatographic method with electrochemical detection for the determination of hyperforin

Rückert, Ulla; Eggenreich, Karin; Wintersteiger, Reinhold; Wurglics, Mario; Likussar, W.; Michelitsch, Astrid

doi:10.1016/j.chroma.2004.04.062

Cited by 16 publications

(5 citation statements)

References 32 publications

(29 reference statements)

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“…The method was applied to the determination of total hypericin (hypericin, pseudohypericin, protohypericin and protopseudohypericin) in herbal extracts after converting the protoforms into hypericin and pseudohypericin by subjecting the samples to artificial light . In the same setting, the limit of detection for hyperforin was 0.05 ng . An improved method with amperometric detection allowed the simultaneous determination of total hypericin (protopseudohypericin, pseudohypericin, protohypericin and hypericin) and hyperforin …”

Section: Electroanalytical Methodsmentioning

confidence: 99%

Quality control of Hypericum perforatum L. analytical challenges and recent progress

Agapouda

Booker

Kiss

et al. 2017

Journal of Pharmacy and Pharmacology

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Objectives The most widely applied qualitative and quantitative analytical methods in the quality control of Hypericum perforatum extracts will be reviewed, including routine analytical tools and most modern approaches. Key findings Biologically active components of H. perforatum are chemically diverse; therefore, different chromatographic and detection methods are required for the comprehensive analysis of St. John's wort extracts. Naphthodianthrones, phloroglucinols and flavonoids are the most widely analysed metabolites of this plant. For routine quality control, detection of major compounds belonging to these groups seems to be sufficient; however, closer characterization requires the detection of minor compounds as well. Conclusions TLC and HPTLC are basic methods in the routine analysis, whereas HPLC‐DAD is the most widely applied method for quantitative analysis due to its versatility. LC‐MS is gaining importance in pharmacokinetic studies due to its sensitivity. Modern approaches, such as DNA barcoding, NIRS and NMR metabolomics, may offer new possibilities for the more detailed characterization of secondary metabolite profile of H. perforatum extracts.

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Section: Electroanalytical Methodsmentioning

confidence: 99%

Quality control of Hypericum perforatum L. analytical challenges and recent progress

Agapouda

Booker

Kiss

et al. 2017

Journal of Pharmacy and Pharmacology

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“…Until now, no analytical method has been published for the determination of MC in plasma. Bioanalytical determinations have been only reported for phloroglucinols like hyperforin and flavons depending on high-pressure liquid chromatography associated with UV light [12][13][14][15][16], fluorescence [15,17,18], electrochemical [19] or mass spectrometric detection [20][21][22]. In order to further explore the clinical potential of MC in terms of pharmacodynamics and pharmacokinetics and to be able to evaluate the putative contribution of MC to the therapeutic effects of myrtle, a sensitive LC/MS/MS method was developed for the determination of MC in human and rat plasma.…”

Section: Determination Of Myrtucommulone From Myrtus Communis In Humamentioning

confidence: 99%

Determination of Myrtucommulone fromMyrtus communisin Human and Rat Plasma by Liquid Chromatography/Tandem Mass Spectrometry

Gerbeth¹,

Meins²,

Werz³

et al. 2010

Planta Med

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Recent studies revealed that the non-prenylated acylphloroglucinol myrtucommulone (MC) from myrtle ( MYRTUS COMMUNIS) potently suppresses the biosynthesis of eicosanoids by direct inhibition of cyclooxygenase-1, microsomal prostaglandin E2 synthase (mPGES)-1, and 5-lipoxygenase at IC₅₀ values in the range of 1 to 29 µM. Moreover, MC showed potent efficacy in animal models of inflammation after intraperitoneal administration. Since the main prerequisite for therapeutic efficacy is sufficient bioavailability, it is important to evaluate whether the concentrations of MC achieved in plasma coincide with the pharmacological active concentrations determined in vitro. For that reason, a sensitive LC/MS/MS method has been developed and validated for the determination of MC in human plasma. This method is based on liquid-liquid extraction of plasma samples with 20 % ethyl acetate in tert-butyl methyl ether using the structurally related acylphloroglucinol hyperforin as the internal standard. Chromatographic separation was achieved on a Gemini C6 Phenyl column using a mixture of acetonitrile/water (85 : 15 v/v) containing 6 mM ammonium formate in a run time of 15 min at a flow rate of 1 mL/min, a column temperature of 40 °C, and an autosampler temperature of 5 °C. Mass spectrometric quantification was carried out in the negative ion mode using electrospray ionization (ESI) and multiple-reaction monitoring (MRM). The most intense [M-H]⁻ MRM transition at m/z 667.4 → m/z 194.9 was used for quantification of MC and the transition at m/z 535.4 to m/z 383.2 was used to monitor hyperforin. The method was linear in the range of 1-100 ng/mL with r > 0.998, an intra- and inter-day RSD of 1.1-8.4 and 7.1-11.8 %, respectively, and a maximum R. E. of 13.8 % at the lowest concentration level. Moreover, cross validation revealed the suitability of the developed LC/MS method for application in rat studies.

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“…[2][3][4] HPLC analysis of different Hypericum species have been initially reported at various times. [5][6][7][8][9][10][11][12][13][14][15][16] Such approaches usually employed only one detector at a time such as; UV-Vis (UVD), [6][7][8][9][10][11][12][14][15][16][17] electrochemical (ECD), [5,14] fluorescence (FLD), [6,13,14] or mass detectors (LC=MS). [8,9,14,16,17] Thus, to our best knowledge, a HPLC method validation study, performing a comparison of three different detector systems simultaneously (HPLC-ECD, HPLC-UVD, and HPLC-FLD), for flavonoid analysis has not been reported yet.…”

Section: Introductionmentioning

confidence: 99%

Development and Validation of a Multidetector HPLC Method for the Determination of Antioxidant Flavonoids of some Hypericum L. Species

Elgin

Konyalιoğlu

Kılınc

2008

Journal of Liquid Chromatography & Related Technologies

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A multidetector approach for the HPLC analysis of methanolic extracts of three Hypericum species is performed under isocratic conditions through a C 18 (ODS) analytical column. Variable wavelength UV-VIS detector (k max : 230 nm), fluorescence detector (ex: 250 nm em: 450 nm), and a programmable electrochemical detector (þ1.30 V vs. Ag=AgCl) were employed simultaneously for detection. The mobile phase used was a combination of methanol and 0.01 M orthophosphoric acid (pH 7) (50:50, v=v). Retention time values were assigned relatively to those of the standards, and the flavonoid contents were quantified by the standard addition method. Rutin, quercetin-3 0 -glucoside (isoquercitrin), luteolin-4 0 -glucoside, quercetin-4 0 -glucoside, quercetin, naringenin, luteolin, and apigenin were chosen as the model compounds to undergo the validation studies with the three different detector systems. For the comparison of the detector performances, analytical method validation parameters were studied and displayed. Defined methods yielded pretty good results regarding linearity, precision (reproducibility), accuracy, limit of detection, and quantification.

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Development of a high-performance liquid chromatographic method with electrochemical detection for the determination of hyperforin

Cited by 16 publications

References 32 publications

Quality control of Hypericum perforatum L. analytical challenges and recent progress

Quality control of Hypericum perforatum L. analytical challenges and recent progress

Determination of Myrtucommulone fromMyrtus communisin Human and Rat Plasma by Liquid Chromatography/Tandem Mass Spectrometry

Development and Validation of a Multidetector HPLC Method for the Determination of Antioxidant Flavonoids of some Hypericum L. Species

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