Quality control of <i>Hypericum perforatum</i> L. analytical challenges and recent progress

Agapouda, Anastasia; Booker, Anthony; Kiss, Tivadar; Hohmann, Judit; Heinrich, Michael; Csupor, Dezső

doi:10.1111/jphp.12711

Cited by 43 publications

(24 citation statements)

References 111 publications

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“…Many reports have shown the applications of the metabarcoding technique in herbal sample validation and pharmacovigilance 4,15,16,18,27,28 due to its cost effectiveness and ability to reveal the plant species diversity within products. However, this technique has not yet been authenticated for use in a regulatory framework for the quality control of herbal drugs 29 .…”

Section: Discussionmentioning

confidence: 99%

DNA metabarcoding to unravel plant species composition in selected herbal medicines on the National List of Essential Medicines (NLEM) of Thailand

Urumarudappa¹,

Tungphatthong²,

Prombutara³

et al. 2020

Sci Rep

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Traditional medicines are widely traded across the globe and have received considerable attention in the recent past, with expectations of heightened demand in the future. However, there are increasing global concerns over admixture, which can affect the quality, safety, and efficacy of herbal medicinal products. In this study, we aimed to use DNA metabarcoding to identify 39 Thai herbal products on the Thai National List of Essential Medicines (NLEM) and assess species composition and admixture. Among the products, 24 samples were in-house-prepared formulations, and 15 samples were registered formulations. In our study, DNA metabarcoding analysis using ITS2 and rbcL barcode regions were employed to identify herbal ingredients mentioned in the products. The nuclear region, ITS2, was able to identify herbal ingredients in the products at the genus- and family-levels in 55% and 63% of cases, respectively. The chloroplast gene, rbcL, enabled genus- and family-level identifications in 58% and 73% of cases, respectively. In addition, plant species were detected in larger numbers (Family identified, absolute %) in registered herbal products than in in-house-prepared formulations. The level of fidelity increases concerns about the reliability of the products. This study highlights that DNA metabarcoding is a useful analytical tool when combined with advanced chemical techniques for the identification of plant species in highly processed, multi-ingredient herbal products.

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Section: Discussionmentioning

confidence: 99%

DNA metabarcoding to unravel plant species composition in selected herbal medicines on the National List of Essential Medicines (NLEM) of Thailand

Urumarudappa¹,

Tungphatthong²,

Prombutara³

et al. 2020

Sci Rep

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“…Eur.) is the cornerstone of the quality control of raw materials and herbal products, that determines analytical procedures to be applied for qualitative and quantitative assays (EDQM, 2014;Agapouda et al, 2017). Ph.…”

Section: Quality Issues and Analytical Challengesmentioning

confidence: 99%

Benefits and Limitations of DNA Barcoding and Metabarcoding in Herbal Product Authentication

Raclariu

Heinrich

Ichim

et al. 2017

Phytochemical Analysis

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150

126

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IntroductionHerbal medicines play an important role globally in the health care sector and in industrialised countries they are often considered as an alternative to mono‐substance medicines. Current quality and authentication assessment methods rely mainly on morphology and analytical phytochemistry‐based methods detailed in pharmacopoeias. Herbal products however are often highly processed with numerous ingredients, and even if these analytical methods are accurate for quality control of specific lead or marker compounds, they are of limited suitability for the authentication of biological ingredients.ObjectiveTo review the benefits and limitations of DNA barcoding and metabarcoding in complementing current herbal product authentication.MethodRecent literature relating to DNA based authentication of medicinal plants, herbal medicines and products are summarised to provide a basic understanding of how DNA barcoding and metabarcoding can be applied to this field.ResultsDifferent methods of quality control and authentication have varying resolution and usefulness along the value chain of these products. DNA barcoding can be used for authenticating products based on single herbal ingredients and DNA metabarcoding for assessment of species diversity in processed products, and both methods should be used in combination with appropriate hyphenated chemical methods for quality control.ConclusionsDNA barcoding and metabarcoding have potential in the context of quality control of both well and poorly regulated supply systems. Standardisation of protocols for DNA barcoding and DNA sequence‐based identification are necessary before DNA‐based biological methods can be implemented as routine analytical approaches and approved by the competent authorities for use in regulated procedures. © 2017 The Authors. Phytochemical Analysis Published by John Wiley & Sons Ltd.

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“…With regards to drug substances, their quality, and analysis, and using Hypericum perforatum L. (St. Johnʼs Wort) as an example, Agapouda et al [31] reviewed the quality control of H. perforatum. This species is one of the most commonly used ones and a large number of herbal medical preparations are used, with many being licensed or registered, most importantly, for treating minor or moderate forms of depression and a range of mood disorders.…”

Section: Pharmacognosy Today -Analytical and Regulatory Challengesmentioning

confidence: 99%

From Pharmacognosia to DNA-Based Medicinal Plant Authentication – Pharmacognosy through the Centuries

Heinrich¹,

Anagnostou

2017

Planta Med

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For centuries, pharmacognosy was essential for the identification, quality, purity, and, until the end of the 18th century, even for the efficacy of medicinal plants. Since the 19th century, it concentrated on authenticity, purity, quality and the analysis of active substances, and was established as an academic branch discipline within pharmacy and continuously developed into a modern, highly sophisticated science. Even though the paradigm in pharmacy changed in the 19th century with the discovery of morphine and concentrated on single substances that could be synthesized fast by the upcoming industry, medicinal plants always remained an important element of the Materia medica, and during the last decades, medicinal plants continue to be a source of remedies, and natural products are an inspiration for new medicine. In this research, pharmacognostic skills remain an essential element, both with regards to identity, quality assurance of botanicals (both herbal medicines and supplements), and the discovery and development of new medicines. Over the years, the specific pharmacognostical tools have changed dramatically, and most recently, DNA-based techniques have become another element of our spectrum of scientific methods.

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Quality control of Hypericum perforatum L. analytical challenges and recent progress

Cited by 43 publications

References 111 publications

DNA metabarcoding to unravel plant species composition in selected herbal medicines on the National List of Essential Medicines (NLEM) of Thailand

DNA metabarcoding to unravel plant species composition in selected herbal medicines on the National List of Essential Medicines (NLEM) of Thailand

Benefits and Limitations of DNA Barcoding and Metabarcoding in Herbal Product Authentication

From Pharmacognosia to DNA-Based Medicinal Plant Authentication – Pharmacognosy through the Centuries

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