Determination of Cell Wall Protein from Selected Feedstuffs and its Relationship with Ruminal Protein Digestibility in Vitro

Abstract

This study was aimed to analyze neutral detergent insoluble crude protein (NDICP) and acid detergent insoluble crude protein (ADICP) contents of various commonly used forage and concentrate feedstuffs in Indonesia. A number of forages and concentrates, i.e. gliricidia, trichantera, indigofera, calliandra, papaya leaves, cassava leaves, leucaena, rapeseed meal, corn gluten feed, soybean meal, copra meal, palm kernel meal, fish and bone meal and wheat bran were subjected to proximate analysis, cell wall nitrogen determination and in vitro rumen fermentation evaluation. Chemical composition analysis was done in duplicate. The in vitro incubation was conducted in 14 treatments and 3 replicates by following a randomized complete block design. Variables measured after the incubation were total volatile fatty acid (VFA), ammonia, dry matter digestibility (DMD), organic matter digestibility (OMD) and crude protein digestibility (CPD). Results revealed that among the forages, cassava leaf had the highest proportion of NDICP while corn gluten feed was the highest among the concentrates. Different from that of NDICP, the highest proportion of ADICP was obtained in papaya leaf and copra meal for the forages and concentrates, respectively. Higher proportion of NDICP tended to decrease CPD (P<0.1). Although higher ADICP apparently tended to decrease CPD as well, the relationship was insignificant due to the higher variation of the dependent variable. It was concluded that cell wall protein proportion in feed may be used as an indicator to determine the quality of protein and its utilization in the rumen.

Increasing the Quality of Agricultural and Plantation Residues using Combination of Fiber Cracking Technology and Urea for Ruminant Feeds

“…Serum bottles were then immediately sealed with butyl rubber stoppers and aluminum crimp seals to start the incubation at 39ºC. Supernatant was collected for determination of volatile fatty acid (VFA) after 24 h by following the description of Jayanegara et al (2016). After that, the residue was further incubated with 75 ml pepsin-HCl 0.2 N solution for another 24 h to quantify in vitro dry matter digestibility (IVDMD) and in vitro organic matter digestibility (IVOMD), and corrected for blanks.…”

Protein Fractionation and Utilization of Soybean and Redbean as Affected by Different Drying Temperature

“…It is consisted of ligninassociated protein, tannin-protein complexes and heat-damaged protein (Licitra et al, 1996). Our previous study revealed a negative relationship between NDICP or ADICP proportion to total CP and in vitro crude protein digestibility (Jayanegara et al, 2016b). Apparently drying temperature at 50 and 60 o C is able to maintain the quality of protein in soybean and redbean.…”

“…In feed evaluation system, such protein is known as NDICP and ADICP (Licitra et al, 1996;Jayanegara et al, 2016b). The NDICP and ADICP is a protein fraction that insoluble in neutral detergent and acid detergent, respectively.…”

Effect of napier grass supplemented with Gliricidia sepium, Sapindus rarak or Hibiscus rosa-sinensis on in vitro rumen fermentation profiles and methanogenesis

“…However, Muetzel et al (2003) stated that saponins from sesbania did not prevent feed protein from being degraded in vitro. Apart from saponin, different protein fractions present in the feed materials also determine ammonia concentration in the rumen (Jayanegara et al, 2016).…”