Protein Fractionation and Utilization of Soybean and Redbean as Affected by Different Drying Temperature

Abstract

The objective of this study was to investigate the effect of drying temperatures on chemical composition, rumen fermentation, and digestibility of soybean and redbean (in vitro). Soybean and redbean were dried in an oven set at four different drying temperatures: 50, 60, 70, and 80 o C for 24 h in three replicates. The dried samples were then grilled and used further for chemical composition determination (proximate analysis, Van Soest analysis, and protein fraction) and in vitro rumen fermentation assay. Parameters measured in the in vitro assay were gas production, digestibility, pH, ammonia, and volatile fatty acids (VFA). Data obtained were analyzed by using analysis of variance and a posthoc test namely Duncan's multiple range test. Results showed that neutral detergent insoluble crude protein (NDICP) content was increased at higher drying temperature (70 or 80 o C) for both soybean and redbean (P<0.05). Similar to NDICP, higher temperature led to a greater acid detergent insoluble crude protein (ADICP) of soybean as well as those of redbean (P<0.05). Higher temperature decreased gas production rate (GPR) of both beans (P<0.05). Drying of soybean at 70 or 80 o C decreased crude protein digestibility (CPD) of soybean more than those of dried at 50 or 60 o C (P<0.05). Higher drying temperature resulted in a lower NH3 concentration of both beans (P<0.05). In conclusion, drying temperature at 50 or 60 o C was considered to be safe to maintain the nutritional quality of soybean and redbean.

AMMONIATION OF RICE STRAW AND SUPPLEMENTATION OF Paraserianthes falcataria AND Sapindus rarak ON IN VITRO RUMEN FERMENTATION AND METHANE PRODUCTION

“…With regard to VFA produced, in contrast to our expectation, total VFA and its individual components did not change by urea treatment. Generally an increase of gas production is accompanied by an increase of total VFA concentration since both parameters are end products of metabolism of rumen microbes, particularly from carbohydrate metabolism (Morvay et al, 2011;Jayanegara et al, 2017). Apparently the insignificancy of total and individual VFA in the present study is due to the high variation among replicates.…”

“…It was confirmed by the increase of gas production and degradability of ammoniated rice straw in comparison to the untreated rice straw. Gas produced in an in vitro rumen fermentation system is originated from two sources; it is an end product of microbial metabolism during feed degradation and fermentation, and as a result of VFA buffering process by bicarbonate buffer present in the incubation medium (Jayanegara et al, 2017). Therefore gas production is considered as an indicator of feed degradability or digestibility.…”

Stability analysis and nutritional quality of soybean (Glycine max (L).Merrill.) genotypes for feed in southwestern Ethiopia

“…The fiber content of a feed is particularly important for determining quality in terms of digestion. NDF content represents most of the cell wall/fiber of soybeans seeds [ 42 ], while ADF content consists of primary lignocellulose structure and is resistant to microbial fermentation in the rumen [ 28 ]. NDF levels have a negative correlation with feed intake, while ADF has a negative correlation with digestibility values of feedstuffs [ 43 ].…”