Detection of virulence genes and antimicrobial susceptibility of salmonella  spp isolated from diarrheal human in wasit province, Iraq

Jassim, Asaad Ali; Al-Gburi, Nagham M.

doi:10.53730/ijhs.v6ns3.7763

Cited by 5 publications

(3 citation statements)

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“…Research addressing the issue of antibacterial resistant bacteria in Iraq includes many works in the recent years. Most researchers focused on bacterial species such as: Salmonella species [36][37][38][39][40][41][42], Campylobacter species [43][44][45], Staphylococcus aureus [46,47] and Escherichia coli [48] and [49].…”

Section: Resultsmentioning

confidence: 99%

Antimicrobial resistance in aerobic bacterial isolates from broiler lungs

Ibrahim¹,

Jaafer²,

Fayyadh³

2022

PEN

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Antibacterial resistant bacteria cause a big concern to poultry and to public health in general because some bacterial poultry pathogens can infect humans or transfer their resistance ability to human pathogens. Nontherapeutic use of antibacterial in poultry, especially as growth promoters to increase feed conversion efficiency is thought to be one of the main causes of resistance. The study included pulmonary swab samples collected during necropsy from 120 poultry farms showing respiratory symptoms with mortality. The disc diffusion method for antibiotic sensitivity testing was performed and antibiotic disks for 21 antibiotics were used. The results showed that three antibacterial were sensitive to more than 50% of the isolates. The first is doxycycline and 69.9% of the isolates were sensitive. The second is Cefalexin with 60.5% sensitive isolates and third is Chloramphenicol with 55.2% sensitive isolates. In the rest of antibacterial, less than 50% were sensitive. Five isolates were found resistant to all antibacterial. Moreover, three samples were found to be negative with no bacterial growth. The present study concluded that 50% of the aerobic bacteria isolated from poultry lungs are resistant to 85% of the 21 antibiotics tested in the study.

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Section: Resultsmentioning

confidence: 99%

Antimicrobial resistance in aerobic bacterial isolates from broiler lungs

Ibrahim¹,

Jaafer²,

Fayyadh³

2022

PEN

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“…Similarly, the stn genes were detected in 71.7% of the tested isolates in Egypt [ 24 ]. On the other hand, the stn gene could be detected in all Salmonella isolates [ 34 , 36 , 37 ]. In contrast,…”

Section: Discussionmentioning

confidence: 99%

Detection of Virulence and β-lactamase resistance genes of non-typhoidal Salmonella isolates from human and animal origin in Egypt "one health concern"

Diab

Thabet²,

Elsalam³

et al. 2023

Gut Pathog

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Background Non-typhoidal Salmonella (NTS) is a major foodborne zoonotic pathogen worldwide. In the current study, Various NTS strains were isolated from (cows, milk and dairy products in addition to humans) in New Valley and Assiut Governorate, Egypt. NTS were firstly serotyped and tested by antibiotic sensitivity test. Secondly, some virulence genes and Antibiotic resistance genes have been identified by using PCR. Finally, Phylogenesis was performed depending on the invA gene, for two S. typhimurium isolates (one of animal origin and the other of human origin for evaluating zoonotic potential). Results Out of 800 examined samples, the total number of isolates was 87 (10.88%), which were classified into 13 serotypes, with the most prevalent being S. Typhimurium and S. enteritidis. Both bovine and human isolates showed the highest resistance to clindamycin and streptomycin, with 90.80% of the tested isolates exhibiting MDR. The occurrence of the invA gene was 100%, while 72.22%, 30.56%, and 94.44% of the examined strains were positive for stn, spvC, and hilA genes, respectively. Additionally, blaOXA-2 was detected in 16.67% (6/ 36) of the tested isolates, while blaCMY-1 was detected in 30.56% (11of 36) of the tested isolates. Phylogenesis revealed a high degree of similarity between the two isolates. Conclusions The high occurrence of MDR strains of NTS in both human and animal samples with high degree of genetic similarity, shows that cows, milk and milk product may be a valuable source of human infection with NTS and interfere with treatment procedures.

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“…Occurrence of NTS in raw milk in this study was higher than the occurrence reported by[20] who investigated that 6.66% of each market raw milk and bulk tank milk was contaminated by NTS. Also, lower occurrence was observed by[21] who isolated NTS from 3% (4 out of 133) of raw market milk samples but they failed to isolate Salmonella from milk samples collected directly from teat of lactating cows. On the other hand,[22] estimated higher occurrence (20.5%) of NTS in raw milk collected directly from the mammary gland during milking than in our study.…”

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confidence: 94%

Occurrence of non-typhoidal Salmonella (NTS) in New Valley and Assiut provinces

Thabet,

Sotohy,

Abd El Salam

et al. 2023

New Valley Veterinary Journal

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The purpose of this study was to investigate the occurrence of non-typhoidal Salmonella (NTS) and the distributed serotypes in New Valley and Assiut provinces. Eight hundred samples were collected (280 from New Valley and 520 from Assiut) from different sources including fecal swabs (160), raw milk (80), Damietta cheese (80), Kareish cheese (80), ice cream (80), yoghurt (80), stool samples (170) and hand swabs (70). Samples were bacteriologically examined then the recovered isolates were serotyped. In New Valley province, occurrence of NTS was 8.57%, 3.33%, 0.00%, 20,00%, 17.14% and 5.71% of milk, Kareish cheese, Damietta cheese, yoghurt, fecal swabs and stool samples, respectively, while, the occurrence of NTS in Assiut was 15.56%, 18.00%, 12.50%, 4.44, 8.75%, 15.56%, 11.43% and 8.00% of milk, Kareish cheese, Damietta cheese, yoghurt, ice cream, fecal swabs, hand swabs and stool samples, respectively. 13 different serotypes were detected in the recovered isolates (87) including S. Typhymurium and S. Enteritidis were the predominant serotypes with an occurrence rate of 24.14% and 20.69%, respectively, followed by S. Tsieve (12.64%), S. Infantis (8.05%), S. Larochelle (9.20%), S. Virchow (5.75%), S. Molade (8.05%), S. Haifa (3.45%), S. Shubra (1.15%), S. Alfort (1.15%), S. Essen (2.30%), S. Apeyeme (1.15%) and S. Heidelberg (2.30%). In conclusion, the high prevalence of the NTS in various dairy products represents an alarm for the public health because of the possibility of transmitting these serovars to consumers, which requires reviewing the methods of control and prevention.

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Detection of virulence genes and antimicrobial susceptibility of salmonella spp isolated from diarrheal human in wasit province, Iraq

Cited by 5 publications

References 0 publications

Antimicrobial resistance in aerobic bacterial isolates from broiler lungs

Antimicrobial resistance in aerobic bacterial isolates from broiler lungs

Detection of Virulence and β-lactamase resistance genes of non-typhoidal Salmonella isolates from human and animal origin in Egypt "one health concern"

Occurrence of non-typhoidal Salmonella (NTS) in New Valley and Assiut provinces

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