This study aimed to isolate Salmonella species from diarrheal human and to determine the antimicrobial susceptibility and virulence-associated genes. A total of 145 human stool samples were from  Wasit province, during the period from November 2018 to August 2019. The isolates were identified according to colony morphology, Gram stain, biochemical, Analytical profile index strip (Api-20E) and serotyping. The antimicrobial susceptibility test was done by utilizing the VITEK-2 Compact system and a disc diffusion method. Salmonella isolates were tested to detect six virulence genes, namely sefA, mgtC, sopB, spvR, Stn and invA by PCR technique. Results showed that Salmonella spp was isolated from 9 out of 145(6.2%), S. Typhimurium 55.55%, S. Typhi 33.33%, and S. Enteritidis 11%. The highest isolation rate of species was in the group aged between 3 to 15 years (10.34%). The gender distribution was 7.14% in males and 4.91% in females. August and March recorded the highest level of isolated Salmonella which was 11.76% and 10%, respectively. Multidrug Antibiotic Resistance Index (MARI) of S. Typhimurium, S. Enteritidis, and S. Typhi were 0.4-0.86, 0.57, and 0.62-0.71, respectively.