Detection of Proteins Possibly Involved in Self-Incompatibility Response in Distylous Buckwheat

Abstract

Buckwheat (Fagopyrum esculentum Moench) is a heterostylous plant displaying heteromorphic sporophytic selfincompatibility (SI). In order to detect proteins involved in SI, pistils from both long and short styles were isolated and then selfed or cross-pollinated. One-dimensional gel electrophoresis revealed that short pistils 2 h after selfing contained an unique 50 kDa protein. In the two-dimensional electrophoresis two distinct groups of proteins possibly involved in SI response were detected in the short, and one in the long pistils.

Ubiquitous aspartic proteinase as an actor in the stress response in buckwheat

“…It is well known that buckwheat presents as a distylous plant with long styles (pin morph) and short styles (thrum morph). Legitimate pollination is achievable only between these two types (Miljuš-ukić et al, 2004). Also, though we expected an increased FeAP9 signal in illegitimate pin-pin pollination, the results showed no visible changes in unpollinated pin flowers, in pin flowers pollinated with pin pollen, or in pin flowers pollinated with thrum pollen (Fig.…”

Self-incompatibility in a distylous species of Rubiaceae: is there a single incompatibility response of the morphs?

“…Differences in general pollen tube morphology between floral morphs have suggested that each morph has a different incompatibility reaction mechanism in Turnera species (Tamari et al 2001). The same was suggested for Fagopyrum esculentum (Polygonaceae), after the investigation of proteins exclusive to each morph (Miljuš-Ðukić et al 2004). However, no anatomical or ultrastructural details of the apparently different interaction in each morph have been described for any heterostylous species.…”

Breeding system variation, genetics and evolution in the Turneraceae

“…(1976), and Shivanna et al . (1981) for Primula , as well as for other distylous species, including Averrhoa carambola (Wong et al ., 1994a) and Fagopyrum esculentum (Miljuš‐Ðukić et al . 2004).…”