Ubiquitous aspartic proteinase as an actor in the stress response in buckwheat

Timotijević, Gordana; Milisavljevic, Мilan; Radović, Svetlana; Konstantinović, Miroslav; Maksimović, Vesna

doi:10.1016/j.jplph.2009.06.017

Cited by 45 publications

(33 citation statements)

References 47 publications

(53 reference statements)

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“…Besides the possibilities that have been discussed above, the differences in specificity/catalytic properties and cellular localization among/between the duplicated genes could also contribute to the development of different biological functions, leading to the observed expression divergence [6,68,69]. It was found that almost all of the AP genes within each pair of paralogs were located in different parts of the cell, with two exceptions of VvAP3 / VvAP4 (both in the nucleus), and VvAP17 / VvAP42 (both in the plasma membrane) (Additional file 11).…”

Section: Discussionmentioning

confidence: 99%

Genome-wide identification, evolutionary and expression analysis of the aspartic protease gene superfamily in grape

Guo

Carole

et al. 2013

BMC Genomics

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BackgroundAspartic proteases (APs) are a large family of proteolytic enzymes found in almost all organisms. In plants, they are involved in many biological processes, such as senescence, stress responses, programmed cell death, and reproduction. Prior to the present study, no grape AP gene(s) had been reported, and their research on woody species was very limited.ResultsIn this study, a total of 50 AP genes (VvAP) were identified in the grape genome, among which 30 contained the complete ASP domain. Synteny analysis within grape indicated that segmental and tandem duplication events contributed to the expansion of the grape AP family. Additional analysis between grape and Arabidopsis demonstrated that several grape AP genes were found in the corresponding syntenic blocks of Arabidopsis, suggesting that these genes arose before the divergence of grape and Arabidopsis. Phylogenetic relationships of the 30 VvAPs with the complete ASP domain and their Arabidopsis orthologs, as well as their gene and protein features were analyzed and their cellular localization was predicted. Moreover, expression profiles of VvAP genes in six different tissues were determined, and their transcript abundance under various stresses and hormone treatments were measured. Twenty-seven VvAP genes were expressed in at least one of the six tissues examined; nineteen VvAPs responded to at least one abiotic stress, 12 VvAPs responded to powdery mildew infection, and most of the VvAPs responded to SA and ABA treatments. Furthermore, integrated synteny and phylogenetic analysis identified orthologous AP genes between grape and Arabidopsis, providing a unique starting point for investigating the function of grape AP genes.ConclusionsThe genome-wide identification, evolutionary and expression analyses of grape AP genes provide a framework for future analysis of AP genes in defining their roles during stress response. Integrated synteny and phylogenetic analyses provide novel insight into the functions of less well-studied genes using information from their better understood orthologs.

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Section: Discussionmentioning

confidence: 99%

Genome-wide identification, evolutionary and expression analysis of the aspartic protease gene superfamily in grape

Guo

Carole

et al. 2013

BMC Genomics

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“…The gene-specific primer sets were designed for real-time PCR, as previously described (Li et al 2010) (Supplementary Table 1). Gene expression was normalized to that of the histone H3 gene, which, in this case, was used as a housekeeping gene (Timotijevic et al 2010). Real-time PCR was performed in triplicate on a MiniOpticon system (Bio-Rad Laboratories; Hercules, CA) with the Quantitect SYBR Green PCR Kit (Qiagen).…”

Section: Seed Sterilization and Germinationmentioning

confidence: 99%

Enhancement of rutin in Fagopyrum esculentum hairy root cultures by the Arabidopsis transcription factor AtMYB12

Park

Thwe

et al. 2011

Biotechnol Lett

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Common buckwheat (Fagopyrum esculentum Moench) is rich in phenolic compounds and may be useful for the treatment of metabolic syndrome in humans. To improve the production of rutin in buckwheat, we overexpressed the flavonol-specific transcription factor, AtMYB12 using Agrobacterium rhizogenes into hairy root culture systems. This induced the expression of flavonoid biosynthetic genes encoding phenylalanine ammonia lyase, cinnamate 4-hydroxylase, 4-coumarate:CoA ligase, chalcone synthase, chalcone isomerase, flavone 3-hydroxylase, flavonoid 3'-hydroxylase, and flavonol synthase. This led to the accumulation of rutin in buckwheat hairy roots up to 0.9 mg/g dry wt. PAP1 expression, however, did not correlate with the production of rutin.

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“…RNA isolation, cDNA synthesis and real-time RT-PCR were performed as described by Timotijevic et al (2010). Primers specific for the FeMT3.10 cDNA clone (GeneBank accession no.…”

Section: Real-time Rt-pcrmentioning

confidence: 99%

Tissue expression analysis of FeMT3, a drought and oxidative stress related metallothionein gene from buckwheat (Fagopyrum esculentum)

Samardžić

Nikolić

Timotijević

et al. 2010

Journal of Plant Physiology

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Ubiquitous aspartic proteinase as an actor in the stress response in buckwheat

Cited by 45 publications

References 47 publications

Genome-wide identification, evolutionary and expression analysis of the aspartic protease gene superfamily in grape

Genome-wide identification, evolutionary and expression analysis of the aspartic protease gene superfamily in grape

Enhancement of rutin in Fagopyrum esculentum hairy root cultures by the Arabidopsis transcription factor AtMYB12

Tissue expression analysis of FeMT3, a drought and oxidative stress related metallothionein gene from buckwheat (Fagopyrum esculentum)

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