Self-incompatibility in a distylous species of Rubiaceae: is there a single incompatibility response of the morphs?

Klein, Denise Espellet; Freitas, Leandro; Cunha, Maura Da

doi:10.1007/s00497-009-0097-0

Cited by 18 publications

(16 citation statements)

References 36 publications

(46 reference statements)

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“…Furthermore, ultrastructural analysis revealed disorganized organelles in stigma cells in contact with incompatible pollen. A similar result was reported by Klein et al (2009) when studying incompatible interactions in Psychotria nuda. The authors observed ultrastructural differences between compatible and incompatible pollen tubes, even in the early stages of pollen tube differentiation after pollination.…”

Section: Discussionsupporting

confidence: 87%

Self-incompatibility in passion fruit: cellular responses in incompatible pollinations

et al. 2014

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Self-incompatibility (SI) is a genetic mechanism in angiosperms that prevents selfing. The SI system in passion fruit (Passiflora edulis Sims) was investigated using hand pollinations. Pollen tube growth was inspected by microscopy, and sequence analysis of potential regulators of this process was carried out. The results revealed that the pollen tubes grew slowly and were often completely arrested in the stigma in an incompatible combination. Under these circumstances the pollen tube was rapidly and significantly rearranged, followed by the rapid deposition of callose in the stigma during the SI response. The structural changes in the pollen grain after an incompatible pollination were investigated using scanning electron microscopy. Furthermore, ultrastructural observations during incompatible interactions showed that the membrane system of the pollen tube was damaged, and fertilisation was not observed or was considerably delayed when compared to compatible interactions. The analysis presented here provides evidence that the passion fruit genome presents similar sequences to those encoding factors involved in SI in different species. These results suggest that, in the SI system of passion fruit, the rejection of an incompatible pollen grain is characterised by drastic structural changes in both pollen and pollen tube.

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Section: Discussionsupporting

confidence: 87%

Self-incompatibility in passion fruit: cellular responses in incompatible pollinations

et al. 2014

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“…In distylous plants, self- and intramorphic incompatibility is commonly observed (Klein et al [2009]). Although we did not test fruit set, hand pollination experiments indicated that P. cephalophora is basically self- and intramorphic incompatible (Table 3).…”

Section: Discussionmentioning

confidence: 99%

Distyly and floral morphology of Psychotria cephalophora (Rubiaceae) on the oceanic Lanyu (Orchid) Island, Taiwan

Watanabe

Yang

Nishihara³

et al. 2015

Bot Stud

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BackgroundPsychotria cephalophora Merr. (Rubiaceae), a shrub in oceanic islands of Taiwan and the Philippines, appears to be distylous, but distyly is usually rare on oceanic islands. To elucidate the functional breeding system of P. cephalophora can improve our understanding of plant reproductive ecology on oceanic islands.ResultsField investigations on Lanyu (Orchid Island) off the coast of southeastern Taiwan revealed the flowers to be distylous with short (S)- and long (L)-styled morphs, with only one morph per individual. Laboratory observations revealed that both morphs had stainable pollen grains and indicated dimorphism in stigmatic papillae and pollen size. In hand pollination experiments, the pollen tubes reached the base of the style in intermorph crossing, whereas they rarely penetrated stylar tissue in intramorph crossing and selfing. Open pollinated S- and L-styled flowers produced fruit.ConclusionsThe results indicate that the breeding system of P. cephalophora is morphologically and functionally distylous.Electronic supplementary materialThe online version of this article (doi:10.1186/s40529-015-0091-9) contains supplementary material, which is available to authorized users.

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“…There are also disparities between the pollen tube tips of the morphs including the apparently intact and in some cases swollen tube tips for long-styled plants in contrast to the often ruptured tips for incompatible short-styled plants. Klein et al (2009) observed a comparable rupture of incompatible pollen tubes of short-but not long-styled plants of Psychotria nuda (Rubiaceae).…”

Section: Characteristics Of Incompatible Pollen Tubes Of the Morphsmentioning

confidence: 93%

“…Based on these observations, Tamari et al (2001) hypothesized that different mechanisms might be involved in the process of incompatible pollen rejection of the morphs. Klein et al (2009) proposed a comparable hypothesis based upon ultrastructural investigations of distylous Psychotria nuda (Rubiaceae).…”

Section: Introductionmentioning

confidence: 99%

“…Some detailed studies of the cytology and incompatibility response of distylous species have been carried out Shivanna et al 1981;Dulberger 1974Dulberger , 1975Dulberger , 1987Dulberger , 1992Webster and Gilmartin 2006;Klein et al 2009). The morphological and fine structural features of the tissues of the stigma and style of Primula vulgaris show no major differences between the morphs except for the greater length of the cells of both the stigma head and style of the long-styled morph .…”

Section: Introductionmentioning

confidence: 99%

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Structure of styles and pollen tubes of distylous Turnera joelii and T. scabra (Turneraceae): are there different mechanisms of incompatibility between the morphs?

Safavian

Shore

2010

Sex Plant Reprod

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We investigate the anatomy and fine structure of styles and pollen tubes of two distylous Turnera species, which possess a heteromorphic self-incompatibility system. We use fluorescence and transmission electron microscopy to provide the first description of the cellular aspects of pollen-pistil interactions and ultrastructural changes to pollen tubes during the self-incompatibility response of the morphs. No obvious ultrastructural differences occur between pistils and compatible pollen tubes. Conspicuous differences were, however, observed between incompatible pollen tubes of the morphs. Incompatible pollen tubes of the long-styled morph always appear to be intact, and pollen tube tips are often highly fluorescent when stained with aniline blue, a stain for callose. Swelling and loss of cristae of mitochondria, and circular rough endoplasmic reticulum, were observed for incompatible pollen tubes of the long-styled morph. For incompatible pollen tubes of the short-styled morph, the tube cell wall apex and plasma membrane often appear to be ruptured and no easily recognizable organelles, such as mitochondria, can be discerned. Our results clearly show ultrastructural differences between the morphs and support the hypothesis that different self-incompatibility mechanisms might operate between them.

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Self-incompatibility in a distylous species of Rubiaceae: is there a single incompatibility response of the morphs?

Cited by 18 publications

References 36 publications

Self-incompatibility in passion fruit: cellular responses in incompatible pollinations

Self-incompatibility in passion fruit: cellular responses in incompatible pollinations

Distyly and floral morphology of Psychotria cephalophora (Rubiaceae) on the oceanic Lanyu (Orchid) Island, Taiwan

Structure of styles and pollen tubes of distylous Turnera joelii and T. scabra (Turneraceae): are there different mechanisms of incompatibility between the morphs?

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