Detection of Norfloxacin in Muscle, Liver and Kidney of Broiler Chicken

Abstract

The present study was undertaken to determine the residues of norfloxacin in muscle, liver and kidney of broiler chicken. On the basis of surveillance study, commonly used antibiotic norfloxacin was selected for quantifying the concentration in broiler chicken meat samples. The HPLC system was equipped with photodiode array and C18 hypersil column. The mobile phase consisting of 1 ml Ortho phosphoric acid (85 per cent v/v), 100 ml water HPLC grade with acitonitrile HPLC grade, 87:12 (v/v) then pH was adjusted to 2.0 with triethylamine HPLC Grade. The results indicated that out of 180 samples analysed, 3 liver samples of target stations, viz. T3, T6 and T10 were found positive for norfloxacin residues with concentrations of 2.00, 2.60 and 2.31 µg/kg, respectively. Samples collected from other target stations did not show residual concentration of norfloxacin in muscle, liver and kidney samples.

An investigation on removal of ciprofloxacin and norfloxacin by phycoremediation with an emphasis on acute toxicity and biochemical composition

“…CIP and NOR concentrations were determined according to the USP 28-NF 23 s supplement using 844 UV–VIS compact ion chromatography equipped with an HPLC column (Hichrom-Alltima 5 µm C-18 with dimensions 250 × 4.6 mm) 30 – 32 . Acetonitrile (15%) and ultrapure water (85%) with pH = 3.2 were used as the mobile phase with a flow rate adjusted to 1 mL/min and 250 µL injection volume 19 .…”

​Screening for Enrofloxacin and Ciprofloxacin Residues in Chicken Liver by Liquid Chromatography Tandem Mass Spectrometry Accompanied by Optimal Liquid-liquid Extraction with Phosphoric Acid

“…Many different conditions of extraction of target analytes from meat and liver matrices were reported by employing simple extraction methods using 5% trichloroacetic acid (Verdon et al, 2005), a mixture of acetonitrile and 0.1 M citrate, 150 mM MgCl 2 (Schneider and Donoghue, 2003), phosphoric acid in acetonitrile (Hermo et al, 2006;Chang et al, 2008), 25% ammonia solution (vol/vol) with acetonitrile (San martin, 2007), Hydrochloric acid (Pena et al, 2010;Marni et al, 2011), combined with solid phase extraction (Chung et al, 2018) and with different solvents like 1% formic acid and 0.01M EDTA (Acaröz and Sözbilir, 2020). Phosphoric acid was used as mobile phase for extraction of Fluroquinolone Norfloxacin by Meena et al (2020).…”