Detection of nepoviruses ofVitis viniferain New Zealand using enzyme-linked immunosorbent assay (ELISA)

Abstract

In addition to grapevine fanleaf virus (GFV), 2 other nepoviruses which had not previously been detected in New Zealand grapevinesarabis mosaic (ArMY) and tobacco ringspot ~TobRV) -were detected using enzyme-linked Immunosorbent assay (ELISA). Using ELISA it was possible to detect infections which were not apparent using mechanical transmission tests. GFV was reliably detected only in grapevine leaf tissue extracted in high molarity phosphate buffer containing both thioglycollic acid and sodium diethyldithiocarbamate. Of the 346 varieties held in a national grapevine variety collection 9% contained only GFV, 10% contained only ArMY, and 15% con tamed both GFV and ArMY. Less than 1% of the varieties contained TobRV alone, and none were infected with all 3 viruses.

Early detection of grapevine leafroll virus in Vitis vinifera using in vitro micrografting

“…However, this method is time consuming and labour-intensive, with a 2-3 year incubation period of the grafted indicators in a nursery. Other methods include serological (Kearns and Mossop, 1984;Garnsey and Cambra, 1993;Monis and Bestwick, 1996) or molecular (Wan Chow Wah and Symons, 1997;Goszczynski and Jooste, 2003) techniques. These are rapid and reliable techniques, but are limited to known viruses for which antibodies and genome sequences are available.…”