Early detection of grapevine leafroll virus in Vitis vinifera using in vitro micrografting

Pathirana, Ranjith; McKenzie, Marian J.

doi:10.1007/s11240-004-2498-y

Cited by 27 publications

(23 citation statements)

References 17 publications

(16 reference statements)

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“…Until now, micrografting has been widely used for elimination of various plant pathogens such as viruses from citrus (Murashige et al ., ; Roistacher et al ., ; Juárez et al ., ; Sanabam et al ., ), apple (Huang & Millikan, ), peach (Jonard et al ., ; Conejero et al ., ), plum (Conejero et al ., ) and sweet pepper (Katoh et al ., ), viroids from citrus (Roistacher et al ., ), peach (Barba et al ., ) and avocado (Suarez et al ., ), and phytoplasma from jujuba (Wang et al ., ). This technique has also been applied to the studies of biology of grafting (Pina & Errea, ; Gökbayrak et al ., ; Melnyk et al ., ), cell signalling (Turnbull et al ., ; Yin et al ., ), rejuvenation (Revilla et al ., ), plant regeneration following micropropagation (Onay et al ., ; Badalamenti et al ., ), genetic transformation (Almeida et al ., ; Juárez et al ., ) and cryopreservation (Volk et al ., ; Wang et al ., ), in vitro breeding (Bisognin et al ., ), and in vitro biological indexing of viruses and viroids (Tanne et al ., ; Zilkah et al ., ; Pathirana & Mckenzie, ; Kapari‐Isaia et al ., ; Wang & Valkonen, ). Applications of micrografting to crop improvement have been extensively reviewed by Jonard et al .…”

Section: Introductionmentioning

confidence: 99%

Development, histological observations and Grapevine leafroll‐associated virus‐3 localisation in in vitro grapevine micrografts

Hao

Cui

et al. 2017

Annals of Applied Biology

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Development, histological process and Grapevine leafroll-associated virus-3 localisation were studied in micrografts of three scion/rootstock combinations: healthy/healthy, healthy/infected and infected/healthy. Earlier bud break and faster growth in scions of micrografts were obtained when the healthy shoot segments were used as scions, while earlier bud break in rootstocks and greater fresh weight of roots in micrografts were produced when the healthy shoot segments were used as rootstocks. All histological processes including callus initiation and formation in micrografting conjunctions, and initiation of new cambial cells followed by vascular bundle development connecting scions and rootstocks were similar in micrografts, regardless of the sanitary status of the scions and rootstocks used for micrografting. Virus infection in micrografting conjunctions and systematic infection in micrografts were much more efficient and faster in micrografting combination of the infected scions/healthy rootstocks than in the healthy scions/infected rootstocks. To the best of our knowledge, this is the first report addressing histological process of micrograft development and virus localisation in micrografts. In vitro culture system established in this study facilitates studies on the 'pure' impact of the viral infection on micrografting.

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Section: Introductionmentioning

confidence: 99%

Development, histological observations and Grapevine leafroll‐associated virus‐3 localisation in in vitro grapevine micrografts

Hao

Cui

et al. 2017

Annals of Applied Biology

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“…This kind of reverse-cleft micrografting technique is a quite good solution for micrografting when rootstock is very thin in Chinese jujube and maybe in other plants. Pathirana and McKenzie (2005) compared the effect of two scion genotypes on two rootstocks in a grapevine micrografting experiment. There were no significant differences between the success rates for the different combinations of rootstock and scion, and the success of the grafts relied completely on how carefully to place the scion on the cleft of the rootstock.…”

Section: Resultsmentioning

confidence: 99%

Reverse-cleft in vitro micrografting of Ziziphus jujuba Mill. Infected with jujube witches’ broom (JWB)

Liu

Ning³

et al. 2011

Plant Cell Tiss Organ Cult

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Jujube witches' broom (JWB) is a destructive disease, caused by a phytoplasma, of Chinese jujube (Ziziphus jujuba Mill.), an important traditional fruit in China and South Korea. Micrografting of plantlets to detect those affected with JWB is difficult due to differences in stem diameter between the scion and rootstock. In this study, an in vitro micrografting technique, reverse-cleft graft, suitable for rootstock thinner than scion was developed and optimized. The JWB phytoplasma was successfully transmitted into the scion from diseased rootstock using this technique as determined by polymerase chain reaction. Micrografted plantlets of Chinese jujube were incubated on Murashige and Skoog medium without plant growth regulators. The survival frequencies of 78-91%, depending on grafting combinations, were obtained.

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“…Sürgün ucu aşılamanın bazı turunçgillerde farklı anaç-kalem kombinasyonları kullanılarak yapıldığı bildirilmiştir (Edriss and Burger, 1984;Kapari-Isaia et al, 2002). Şaraplık üzüm çeşitlerinde anaç ve çeşitlerde yapılan farklı kombinasyonlarda in vitro aşılamada fazla bir farklılık görülmezken, in vivo yapılan aşılamalarda büyük farklılıkların olduğu görülmüştür (Pathirana and McKenzie, 2005).…”

Section: Aşı Kalemi a Tür -çEşit Etkisiunclassified

In vitro Micrografting of Woody Plant Species-I (Rootstock, Scion, Grafting Technique)

Çalar¹,

Onay²,

Karlıdağ³

et al. 2017

jist

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ÖZET: İn-vitro aşılama, aksenik kültür koşullarında minyatür aşı kalemlerinin aşılanmasını kapsayan ve diğer tekniklere nazaran en son vejetatif çoğaltım tekniklerinden biridir. Bu yöntem, sürgün ucu kültürü ve aşılamanın bazı sınırlayıcı özelliklerinin üstesinden gelmekle birlikte, her iki metodun avantajlarını da bir arada bulundurmaktadır. İlk uygulandığı zamanlarda bazı meyve tür ve çeşitlerindeki virüs ve benzeri endojen patojenlerin eradikasyonu için geliştirilen in-vitro aşılama, bitki gelişim ve fizyolojisinin farklı alanlarında çeşitli odunsu bitki türlerinde hızla gelişmiştir. Bunlar birçok odunsu türlerin olgun genotiplerinin klonal çoğaltımında bir ön koşul olarak fizyolojik rejuvenasyonu ve aşıda uyuşmazlığı da kapsamaktadır. Sonuç olarak in-vitro aşılama, yoğun olarak kullanılan ve diğer vejatatif çoğaltım metodlarında bulunan olumsuzlukların üstesinden gelebilmek için daha çok düşünülmeyi ve kullanılmayı hak eden orijinal ve tecrübe gerektiren bir tekniktir. Doku ve hücreler arasındaki genetik benzerlik ve farklılıkların ayrıntılı incelenmesine imkan sağlamaktadır. Bu çalışmanın amacı, 1970'li yıllarda turunçgillerde virüsten ari bitki üretimi amacıyla başlayan in vitro aşılama çalışmalarının günümüze kadar nasıl bir süreçten geçtiği ve özellikle meyve türlerinin de dahil olduğu odunsu bitki türlerinde bitki ıslahı ve çoğaltımı amacıyla ne tür çalışmaların yapıldığının ortaya çıkarılması ve belli bir düzende sunulmasıdır.Anahtar Kelimeler: Aşılama, in-vitro, kalem ABSTRACT: In vitro micrografting is a propagation technique, involving the grafting of relatively miniature cuttings under axenic culture conditions and it is one of the recent developed propagation techniques compared to other conventional vegetative propagation techniques. This method overcomes some of the limitations of shoot tip culture and grafting, while it also keeps together the advantages of both methods. Micrografting was applied for the eradication viruses and pathogens from some fruit species and cultivars during the first application period, but later, the technique was further developed on various woody plant species in different research areas of plant physiology and development. These includes physiological rejuvenation and incompatibility grafting as a prerequisite for the clonally propagation of mature genotypes of many woody species. Consequently, in vitro micrografting is used in large scale propagation and an original technique which needs experience by overcoming the disadvantages of other propagation technique. It also enables to examine in detail the genetic similarities and differences between the tissues and cells. The aims of this study were (1) to review how micrografting studies passed a process from 1970s until today, first started to obtain virus-free plants from citrus; (2) to reveal what kind of work has been presented particularly on the plant breeding and propagation of the woody plant species, including the type of fruit breeding and reproduction and (3) and to present those studies in a specific order.

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Early detection of grapevine leafroll virus in Vitis vinifera using in vitro micrografting

Cited by 27 publications

References 17 publications

Development, histological observations and Grapevine leafroll‐associated virus‐3 localisation in in vitro grapevine micrografts

Development, histological observations and Grapevine leafroll‐associated virus‐3 localisation in in vitro grapevine micrografts

Reverse-cleft in vitro micrografting of Ziziphus jujuba Mill. Infected with jujube witches’ broom (JWB)

In vitro Micrografting of Woody Plant Species-I (Rootstock, Scion, Grafting Technique)

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