In addition to grapevine fanleaf virus (GFV), 2 other nepoviruses which had not previously been detected in New Zealand grapevinesarabis mosaic (ArMY) and tobacco ringspot ~TobRV) -were detected using enzyme-linked Immunosorbent assay (ELISA). Using ELISA it was possible to detect infections which were not apparent using mechanical transmission tests. GFV was reliably detected only in grapevine leaf tissue extracted in high molarity phosphate buffer containing both thioglycollic acid and sodium diethyldithiocarbamate. Of the 346 varieties held in a national grapevine variety collection 9% contained only GFV, 10% contained only ArMY, and 15% con tamed both GFV and ArMY. Less than 1% of the varieties contained TobRV alone, and none were infected with all 3 viruses.
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