Detection of multi-β-agonist residues in liver matrix by use of a surface plasma resonance biosensor

Spectral surface plasmon resonance imaging for the detection of clenbuterol via three-dimensional immobilization of bioprobes

“…However, our LOD was larger than the previous ones reported by Li and coworkers [21] using a homemade SPR instrument and by Traynor and coworkers [22] using BIACORE Q. This can be explained by differences in the measurement processes of the two instruments.…”

“…Relationship between the shift in resonance wavelength (dk 2 ) and the concentration of CLEN (target analyte) in the mixture. experiment requires much smaller volumes of samples (10 ll) at higher concentrations, whereas higher sample volumes at lower concentrations are injected into the liquid flow systems of Li and coworkers [21] and Traynor and coworkers [22]. Because of the features of multichannel measurement and the new bioprobe immobilization strategy, the developed method is suitable for fast screening of a large number of samples.…”

“…However, considering that the CLEN molecule contains an amino functional group, it can be immobilized on a sensor chip as a bioprobe via an amide coupling method. Traynor and coworkers [22] reported that CLEN can be immobilized directly on a sensor chip. Even though it saves time and reduces the experiment cost, the bioprobes have a twodimensional structure, with only one orientation for antibodies to bind.…”

Broad screening and identification of β-agonists in feed and animal body fluid and tissues using ultra-high performance liquid chromatography-quadrupole-orbitrap high resolution mass spectrometry combined with spectra library search

“…These methods include liquid chromatography (Chang, Chou, & Hwang, 2005;Meng et al, 2012;Turberg, Macy, Lewis, & Coleman, 1994), gas chromatography (Bocca, Fiori, Cartoni, & Brambilla, 2003), liquid chromatography tandem mass spectrometry (LC-MS/MS) (Shishani, Chai, Jamokha, Aznar, & Hoffman, 2003;Wu, Liu, & Peng, 2014), gas chromatography-mass spectrometry (Montrade, Lebizec, Monteau, & Andre, 1995;Wang, Li, Zhang, & Shen, 2006), enzyme-linked immunoassay (Hahnau & Julicher, 1996;Harkins, Woods, Lehner, Fisher, & Tobin, 2001;Pinheiro et al, 2008), colloidal gold test strip (Khamta, Pattarawarapan, Nangola, & Tayapiwatana, 2009;Zhang et al, 2009), surface plasmon resonance immunosensors (Traynor, Crooks, Bowers, & Elliott, 2003), and electrochemical immunosensors (He, Wang, Zhang, & Yang, 2009). Table 1 Chemical formula, accurate mass, retention time and characteristic fragment ions of 32 b-agonists.…”

A review on novel developments and applications of immunosensors in food analysis

“…A high-affinity, broadspectrum ␤-agonist monoclonal antibody was applied in a SPR immuno-biosensor competitive assay (Fig. 1D) [106]. Also in this case, the sample treatment was quite complex and included proteolytic digestion, enzymatic deconjugation and solid-phase extraction.…”