A review on novel developments and applications of immunosensors in food analysis

Ricci, Francesco; Volpe, G.; Micheli, Laura; Palleschi, Giuseppe

doi:10.1016/j.aca.2007.10.046

Cited by 310 publications

(147 citation statements)

References 127 publications

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“…As molecular recognition is the foundation of biosensing, there has been increased focus on the development of new molecular recognition probes for food-safety related molecular targets. Antibodies have been the gold standard in molecular recognition for several decades and have been incorporated widely into biosensors and assays relating to food (Ricci et al, 2007). Despite their applicability to food monitoring, they are not without their disadvantages, which are primarily linked to the requirement that antibody generation is an in vivo process.…”

Section: Introductionmentioning

confidence: 99%

Advances in Aptamer-Based Biosensors for Food Safety

McKeague¹,

Giamberardino²,

DeRosa³

2011

Environmental Biosensors

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Section: Introductionmentioning

confidence: 99%

Advances in Aptamer-Based Biosensors for Food Safety

McKeague¹,

Giamberardino²,

DeRosa³

2011

Environmental Biosensors

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“…The sensitivity of the developed immunosensor was not as high as other previously reported immunosensors targeting different analytes (Piermarini et al, 2007;Ricci et al, 2007). This, however, cannot be ascribed to the fact that a Fab fragment is used instead of a natural antibody.…”

Section: Resultsmentioning

confidence: 68%

“…The possible alternative use of electrochemical technique to develop sensitive immunosensors with similar or even better performances of the more classic spectrophotometric ELISA assay has been demonstrated by several groups (reviewed in Ricci et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

Development of a recombinant Fab-fragment based electrochemical immunosensor for deoxynivalenol detection in food samples

Romanazzo

Ricci

Volpe

et al. 2010

Biosensors and Bioelectronics

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a b s t r a c tA reliable and cost-effective electrochemical method for the detection of deoxynivalenol (DON) in cereals and cereal-based food samples based on the use of a novel anti-DON Fab fragment is presented. The analytical system employed, Enzyme-Linked-Immunomagnetic-Electrochemical (ELIME) assay, is based on the use of immunomagnetic beads (IMBs) coupled with eight magnetized screen-printed electrodes (8-mScPEs) as electrochemical transducers.Using standard solutions of DON, a working range between 100 and 4500 ng/ml was obtained with an EC 50 of 380 ng/ml. The ELIME assay was employed to evaluate the cross-reactivity of the Fab fragment towards different trichothecenes revealing a good selectivity towards DON over other trichothecenes with the exception of 3-Ac-DON. The sensor was then applied to cereals and cereal-based food samples (wheat, breakfast cereal and baby-food) and a wide range of sample treatment procedures was tested. Within-laboratory precision (9-24% repeatability for breakfast cereals and 10-33% for baby-food) and recovery data (82-110% for breakfast cereals and 97-108% for baby-food) were calculated by analyzing blank breakfast cereals and baby-foods fortified with DON, demonstrating that the proposed method has the capability for use as a screening assay for DON in such products.

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“…Excellent reviews are available for various rapid detection methods including biosensors for determination of drug residues in meat (Reig and Toldra 2008) and SPR biosensors for antibiotics, β-agonists and antiparasitic drugs (Haughey and Baxter 2006). Applications of electrochemical, SPR and quartz crystal microbalance techniques for food analysis were reviewed by Ricci et al (2007).…”

Section: Detection Of Pesticide Residuesmentioning

confidence: 99%

Optical biosensors for food quality and safety assurance—a review

et al. 2011

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Food quality and safety is a scientific discipline describing handling, preparation and storage of food in ways that prevent food borne illness. Food serves as a growth medium for microorganisms that can be pathogenic or cause food spoilage. Therefore, it is imperative to have stringent laws and standards for the preparation, packaging and transportation of food. The conventional methods for detection of food contamination based on culturing, colony counting, chromatography and immunoassay are tedious and time consuming while biosensors have overcome some of these disadvantages. There is growing interest in biosensors due to high specificity, convenience and quick response. Optical biosensors show greater potential for the detection of pathogens, pesticide and drug residues, hygiene monitoring, heavy metals and other toxic substances in the food to check whether it is safe for consumption or not. This review focuses on optical biosensors, the recent developments in the associated instrumentation with emphasis on fiber optic and surface plasmon resonance (SPR) based biosensors for detecting a range of analytes in food samples, the major advantages and challenges associated with optical biosensors. It also briefly covers the different methods employed for the immobilization of bio-molecules used in developing biosensors.

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A review on novel developments and applications of immunosensors in food analysis

Cited by 310 publications

References 127 publications

Advances in Aptamer-Based Biosensors for Food Safety

Advances in Aptamer-Based Biosensors for Food Safety

Development of a recombinant Fab-fragment based electrochemical immunosensor for deoxynivalenol detection in food samples

Optical biosensors for food quality and safety assurance—a review

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