Advances in Aptamer-Based Biosensors for Food Safety

McKeague, Maureen; Giamberardino, Amanda; DeRosa, Maria C.

doi:10.5772/22350

Cited by 29 publications

(26 citation statements)

References 68 publications

(55 reference statements)

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“…In vivo conditions hamper freedom in manipulating processes and can lead to significant batch-to-batch variability (Keefe et al, 2010). Moreover, the shelf lives of antibodies can prevent their effective integration into biosensor platforms (McKeague et al, 2011). Alternatively, DNA aptamers are produced by in vitro selection of functional nucleic acids through a process called Systematic Evolution of Ligands by Exponential enrichment (SELEX) (Stoltenburg et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

Optimisation of an electrochemical impedance spectroscopy aptasensor by exploiting quartz crystal microbalance with dissipation signals

Formisano

Jolly

Bhalla

et al. 2015

Sensors and Actuators B: Chemical

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The response of an Electrochemical Impedance Spectroscopy (EIS) sensor using DNA aptamers is affected by many factors such as DNA density, charge and conformational changes upon DNA-target binding and buffer conditions. We report here for the first time on the optimisation of an EIS aptamerbased sensor by using Quartz Crystal Microbalance with Dissipation mode (QCM-D). As a case study we employed a DNA aptamer against Prostate Specific Antigen (PSA). PSA detection was achieved by functionalizing the gold sensor surface via thiol chemistry with different ratios of thiolated-DNA aptamer and 6-mercapto-1-hexanol (MCH) used as spacer molecules. PSA binding efficiency can be monitored by measuring QCM-D signals which not only provides information about the mass of PSA bound on the sensor surface but also crucial information about the aptamer conformation and layer hydration.Data generated through QCM-D analysis provided the optimal conditions in terms of aptamer/MCH ratio to maximize the PSA binding. The ratio of 1:200 for DNA aptamer/spacer molecule was found to be optimal for ensuring maximum PSA binding. However, this study showed how a maximum analyte binding does not necessarily correspond to a maximum EIS response, which revealed to be enhanced if a ratio of 1:100 for DNA aptamer/spacer molecule was used. Moreover, by monitoring the QCM-D signal, for the first time a value of the dissociation constant (K d ), equal to 37 nM, was found for the PSA DNA aptamer towards its target. The combination of QCM-D with EIS techniques provide further insight into the effects of mass loading and charge effects that govern the response of an EIS aptasensor, serving as a valuable support for future EIS aptamer-based applications.

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Section: Introductionmentioning

confidence: 99%

Optimisation of an electrochemical impedance spectroscopy aptasensor by exploiting quartz crystal microbalance with dissipation signals

Formisano

Jolly

Bhalla

et al. 2015

Sensors and Actuators B: Chemical

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“…For food screening, combinatorially derived nucleic acids (aptamers) (Potyrailo, Conrad, Ellington, & Hieftje, 1998) have been synthesized and selected for interaction with specific targets relevant to food safety and defense; many have demonstrated binding capabilities in complex matrices (McKeague, Giamberardino, & Derosa, 2011). An evanescent wave fiber-optic biosensor using aptamers for the capture of Listeria monocytogenes was recently described; cells could be detected directly from ready-to-eat meats or after an 18 hour enrichment step (Ohk et al, 2010).…”

Section: Development and Integration Of Alternative Recognition Speciesmentioning

confidence: 99%

Total internal reflection fluorescence (TIRF) array biosensors for biothreat agents for food safety and food defense

Taitt¹,

North²

2015

High Throughput Screening for Food Safety Assessment

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“…Currently, a major focus in using these transduction materials for sensing is on hybrid nanocomposites containing carbon nanomaterials (e.g., graphene) and metal or metal oxide nanostructures. Because of their excellent molecular recognition properties, capture agents such as lectins, antibodies, and aptamers are being used to construct high fidelity biosensors 14 . Aptamers are nucleic-acid molecules artificially generated to detect specific targets such as a particular species of bacteria.…”

Section: Introductionmentioning

confidence: 99%

Rapid detection of listeria spp. using an internalin A aptasensor based on carbon-metal nanohybrid structures

et al. 2015

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Foodborne outbreaks caused by Listeria monocytogenes continue to raise major public health concerns worldwide. In the United States alone, the centers for disease control and prevention have confirmed the occurrence of 183 cases of listeriosis with 39 fatalities within the last 3 years. Standard methods for the detection of pathogenic strains require up to 7 days to yield results, thus faster techniques with the same level of reliability for bacteria detection are desirable. This study reports on the development of a rapid, accurate, and sensitive electrochemical biosensor for rapid testing of Listeria spp. based on the selective binding of InlA aptamers to internalins in the cell membrane of the target bacteria. Hybrid nanomaterial platforms based on reduced graphene oxide and nanoplatinum were deposited onto Pt/Ir electrodes for enhancing electrochemical transduction during the recognition events. InlA aptamers were immobilized onto the nanomaterial platforms via metal-thiol adsorption. Aptamer loading onto different platform nanostructures was investigated through cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). The detection mechanism was evaluated by recording the electrochemical response to several bacterial dilutions in PBS buffer using the non-pathogenic species Listeria innocua. These preliminary results show that the aptasensor can be tuned for detection of Listeria concentrations as low as 100 CFU/ml in less than 3 hours (including incubation time and data analysis). The developed aptasensor opens a promising direction for rapid testing of Listeria monocytogenes in food products.

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Advances in Aptamer-Based Biosensors for Food Safety

Cited by 29 publications

References 68 publications

Optimisation of an electrochemical impedance spectroscopy aptasensor by exploiting quartz crystal microbalance with dissipation signals

Optimisation of an electrochemical impedance spectroscopy aptasensor by exploiting quartz crystal microbalance with dissipation signals

Total internal reflection fluorescence (TIRF) array biosensors for biothreat agents for food safety and food defense

Rapid detection of listeria spp. using an internalin A aptasensor based on carbon-metal nanohybrid structures

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